7p4v - Revision history

OCA at 13:01, 1 February 2024

OCA — Thu, 01 Feb 2024 13:01:30 GMT

←Older revision		Revision as of 13:01, 1 February 2024
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	==GlnK1 from Methanothermococcus thermolithotrophicus with dADP at a resolution of 1.94 A==		==GlnK1 from Methanothermococcus thermolithotrophicus with dADP at a resolution of 1.94 A==
-	<StructureSection load='7p4v' size='340' side='right'caption='[[7p4v]]' scene=''>	+	<StructureSection load='7p4v' size='340' side='right'caption='[[7p4v]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P4V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P4V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7p4v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus_DSM_2095 Methanothermococcus thermolithotrophicus DSM 2095]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P4V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P4V FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p4v OCA], [https://pdbe.org/7p4v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p4v RCSB], [https://www.ebi.ac.uk/pdbsum/7p4v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p4v ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.94Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DAT:2-DEOXYADENOSINE-5-DIPHOSPHATE'>DAT</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p4v OCA], [https://pdbe.org/7p4v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p4v RCSB], [https://www.ebi.ac.uk/pdbsum/7p4v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p4v ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein inhibition is a natural regulatory process to control cellular metabolic fluxes. PII-family signal-transducing effectors are in this matter key regulators of the nitrogen metabolism. Their interaction with their various targets is governed by the cellular nitrogen level and the energy charge. Structural studies on GlnK, a PII-family inhibitor of the ammonium transporters (Amt), showed that the T-loops responsible for channel obstruction are displaced upon the binding of 2-oxoglutarate, magnesium and ATP in a conserved cleft. However, GlnK from Methanocaldococcus jannaschii was shown to bind 2-oxoglutarate on the tip of its T-loop, causing a moderate disruption to GlnK-Amt interaction, raising the question if methanogenic archaea use a singular adaptive strategy. Here we show that membrane fractions of Methanothermococcus thermolithotrophicus released GlnKs only in the presence of Mg-ATP and 2-oxoglutarate. This observation led us to structurally characterize the two GlnK isoforms apo or in complex with ligands. Together, our results show that the 2-oxoglutarate binding interface is conserved in GlnKs from Methanococcales, including Methanocaldococcus jannaschii, emphasizing the importance of a free carboxy-terminal group to facilitate ligand binding and to provoke the shift of the T-loop positions.
		+
		+	The Oxoglutarate Binding Site and Regulatory Mechanism Are Conserved in Ammonium Transporter Inhibitors GlnKs from Methanococcales.,Muller MC, Wagner T Int J Mol Sci. 2021 Aug 11;22(16). pii: ijms22168631. doi: 10.3390/ijms22168631. PMID:34445335<ref>PMID:34445335</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7p4v" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Methanothermococcus thermolithotrophicus DSM 2095]]
	[[Category: Mueller M-C]]		[[Category: Mueller M-C]]
	[[Category: Wagner T]]		[[Category: Wagner T]]

OCA at 05:33, 6 October 2021

OCA — Wed, 06 Oct 2021 05:33:21 GMT

←Older revision		Revision as of 05:33, 6 October 2021
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-	'''Unreleased structure'''

-	~~The entry 7p4v is ON HOLD until Paper Publication~~	+	==GlnK1 from Methanothermococcus thermolithotrophicus with dADP at a resolution of 1.94 A==
-		+	<StructureSection load='7p4v' size='340' side='right'caption='[[7p4v]]' scene=''>
-	~~Authors: Mueller, M.-C., Wagner, T.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P4V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P4V FirstGlance]. <br>
-	~~Description:~~ GlnK1 from Methanothermococcus thermolithotrophicus with dADP at a resolution of 1.94 A	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p4v OCA], [https://pdbe.org/7p4v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p4v RCSB], [https://www.ebi.ac.uk/pdbsum/7p4v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p4v ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Wagner~~, T]]	+	__TOC__
-	[[Category: Mueller, M.-C]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mueller M-C]]
		+	[[Category: Wagner T]]

OCA at 05:02, 11 August 2021

OCA — Wed, 11 Aug 2021 05:02:58 GMT

←Older revision		Revision as of 05:02, 11 August 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7p4v is ON HOLD	+	The entry 7p4v is ON HOLD until Paper Publication

	Authors: Mueller, M.-C., Wagner, T.		Authors: Mueller, M.-C., Wagner, T.

OCA: Protected "7p4v" [edit=sysop:move=sysop]

OCA — Wed, 21 Jul 2021 07:56:08 GMT

Protected "7p4v" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:56, 21 July 2021

OCA: New page: '''Unreleased structure''' The entry 7p4v is ON HOLD Authors: Mueller, M.-C., Wagner, T. Description: GlnK1 from Methanothermococcus thermolithotrophicus with dADP at a resolution of 1...

OCA — Wed, 21 Jul 2021 07:56:07 GMT

New page: '''Unreleased structure''' The entry 7p4v is ON HOLD Authors: Mueller, M.-C., Wagner, T. Description: GlnK1 from Methanothermococcus thermolithotrophicus with dADP at a resolution of 1...

New page

'''Unreleased structure'''

The entry 7p4v is ON HOLD

Authors: Mueller, M.-C., Wagner, T.

Description: GlnK1 from Methanothermococcus thermolithotrophicus with dADP at a resolution of 1.94 A
[[Category: Unreleased Structures]]
[[Category: Wagner, T]]
[[Category: Mueller, M.-C]]