OCA at 06:52, 14 September 2022

OCA — Wed, 14 Sep 2022 06:52:23 GMT

←Older revision		Revision as of 06:52, 14 September 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7p5h is ~~ON HOLD until Paper~~ Publication	+	==TmHydABC- D2 map==
		+	<StructureSection load='7p5h' size='340' side='right'caption='[[7p5h]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7p5h]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P5H FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p5h OCA], [https://pdbe.org/7p5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p5h RCSB], [https://www.ebi.ac.uk/pdbsum/7p5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p5h ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/Q9S5X7_THEMA Q9S5X7_THEMA]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Electron-bifurcation is a fundamental energy conservation mechanism in nature in which two electrons from an intermediate potential electron donor are split so that one is sent along a high potential pathway to a high potential acceptor and the other is sent along a low potential pathway to a low potential acceptor. This process allows endergonic reactions to be driven by exergonic ones and is an alternative, less recognised, mechanism of energy coupling to the well-known chemiosmotic principle. The electron-bifurcating [FeFe] hydrogenase from Thermotoga maritima (HydABC) requires both NADH and ferredoxin to reduce protons generating hydrogen. The mechanism of electron-bifurcation in HydABC remains enigmatic in spite of intense research efforts over the last few years. Structural information may provide the basis for a better understanding of spectroscopic and functional information. Here, we present a 2.3 A electron cryo-microscopy structure of HydABC. The structure shows a heterododecamer composed of two independent 'halves' each made of two strongly interacting HydABC heterotrimers connected via a [4Fe-4S] cluster. A central electron transfer pathway connects the active sites for NADH oxidation and for proton reduction. We identified two conformations of a flexible iron-sulfur cluster domain: a 'closed bridge' and an 'open bridge' conformation, where a Zn(2+) site may act as a 'hinge' allowing domain movement. Based on these structural revelations, we propose a possible mechanism of electron-bifurcation in HydABC where the flavin mononucleotide serves a dual role as both the electron bifurcation center and as the NAD(+) reduction/NADH oxidation site.

-	~~Authors~~:	+	Structural insight on the mechanism of an electron-bifurcating [FeFe] hydrogenase.,Furlan C, Chongdar N, Gupta P, Lubitz W, Ogata H, Blaza JN, Birrell JA Elife. 2022 Aug 26;11. pii: 79361. doi: 10.7554/eLife.79361. PMID:36018003<ref>PMID:36018003</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7p5h" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Thermotoga maritima MSB8]]
		+	[[Category: Birrell JA]]
		+	[[Category: Blaza JN]]
		+	[[Category: Chongdar N]]
		+	[[Category: Furlan C]]
		+	[[Category: Gupta P]]
		+	[[Category: Lubitz W]]
		+	[[Category: Ogata H]]

OCA at 12:38, 3 November 2021

OCA — Wed, 03 Nov 2021 12:38:07 GMT

←Older revision		Revision as of 12:38, 3 November 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7p5h is ON HOLD	+	The entry 7p5h is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7p5h" [edit=sysop:move=sysop]

OCA — Wed, 21 Jul 2021 07:57:08 GMT

Protected "7p5h" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:57, 21 July 2021

OCA: New page: '''Unreleased structure''' The entry 7p5h is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 21 Jul 2021 07:57:07 GMT

New page: '''Unreleased structure''' The entry 7p5h is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7p5h is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7p5h - Revision history

OCA at 06:52, 14 September 2022

OCA at 12:38, 3 November 2021

OCA: Protected "7p5h" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7p5h is ON HOLD Authors: Description: Category: Unreleased Structures