OCA at 13:01, 1 February 2024

OCA — Thu, 01 Feb 2024 13:01:43 GMT

←Older revision		Revision as of 13:01, 1 February 2024
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	==1.93 A resolution X-ray crystal structure of the transcriptional regulator SrnR from Streptomyces griseus==		==1.93 A resolution X-ray crystal structure of the transcriptional regulator SrnR from Streptomyces griseus==
-	<StructureSection load='7p6f' size='340' side='right'caption='[[7p6f]]' scene=''>	+	<StructureSection load='7p6f' size='340' side='right'caption='[[7p6f]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P6F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P6F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7p6f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P6F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P6F FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p6f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p6f OCA], [https://pdbe.org/7p6f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p6f RCSB], [https://www.ebi.ac.uk/pdbsum/7p6f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p6f ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.93Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p6f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p6f OCA], [https://pdbe.org/7p6f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p6f RCSB], [https://www.ebi.ac.uk/pdbsum/7p6f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p6f ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8L1Y3_STRGR Q8L1Y3_STRGR]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Streptomyces griseus, a bacterium producing antibacterial drugs and featuring possible application in phytoremediation, expresses two metal-dependent superoxide dismutase (SOD) enzymes, containing either Fe(II) or Ni(II) in their active site. In particular, the alternative expression of the two proteins occurs in a metal-dependent mode, with the Fe(II)-enzyme gene (sodF) repressed at high intracellular Ni(II) concentrations by a two-component system (TCS). This complex involves two proteins, namely SgSrnR and SgSrnQ, which represent the transcriptional regulator and the Ni(II) sensor of the system, respectively. SgSrnR belongs to the ArsR/SmtB family of metal-dependent transcription factors; in the apo-form and in the absence of SgSrnQ, it can bind the DNA operator of sodF, upregulating gene transcription. According to a recently proposed hypothesis, Ni(II) binding to SgSrnQ would promote its interaction with SgSrnR, causing the release of the complex from DNA and the consequent downregulation of the sodF expression. SgSrnQ is predicted to be highly disordered, thus the understanding, at the molecular level, of how the SgSrnR/SgSrnQ TCS specifically responds to Ni(II) requires the knowledge of the structural, dynamic, and functional features of SgSrnR. These were investigated synergistically in this work using X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, atomistic molecular dynamics calculations, isothermal titration calorimetry, and in silico molecular docking. The results reveal that the homodimeric apo-SgSrnR binds to its operator in a two-step process that involves the more rigid globular portion of the protein and leaves its largely disordered regions available to possibly interact with the disordered SgSrnQ in a Ni-dependent process.
		+
		+	Structure, dynamics, and function of SrnR, a transcription factor for nickel-dependent gene expression.,Mazzei L, Musiani F, Zerko S, Kozminski W, Cianci M, Beniamino Y, Ciurli S, Zambelli B Metallomics. 2021 Dec 20;13(12). pii: 6445039. doi: 10.1093/mtomcs/mfab069. PMID:34850061<ref>PMID:34850061</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7p6f" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Streptomyces griseus]]
	[[Category: Ciurli S]]		[[Category: Ciurli S]]
	[[Category: Mazzei L]]		[[Category: Mazzei L]]

OCA at 07:56, 25 May 2022

OCA — Wed, 25 May 2022 07:56:47 GMT

←Older revision		Revision as of 07:56, 25 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7p6f ~~is ON HOLD until Paper Publication~~	+	==1.93 A resolution X-ray crystal structure of the transcriptional regulator SrnR from Streptomyces griseus==
-		+	<StructureSection load='7p6f' size='340' side='right'caption='[[7p6f]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P6F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P6F FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p6f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p6f OCA], [https://pdbe.org/7p6f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p6f RCSB], [https://www.ebi.ac.uk/pdbsum/7p6f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p6f ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ciurli S]]
		+	[[Category: Mazzei L]]

OCA at 09:29, 28 July 2021

OCA — Wed, 28 Jul 2021 09:29:20 GMT

←Older revision		Revision as of 09:29, 28 July 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7p6f is ON HOLD	+	The entry 7p6f is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7p6f" [edit=sysop:move=sysop]

OCA — Wed, 21 Jul 2021 07:58:45 GMT

Protected "7p6f" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:58, 21 July 2021

OCA: New page: '''Unreleased structure''' The entry 7p6f is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 21 Jul 2021 07:58:44 GMT

New page: '''Unreleased structure''' The entry 7p6f is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7p6f is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7p6f - Revision history

OCA at 13:01, 1 February 2024

OCA at 07:56, 25 May 2022

OCA at 09:29, 28 July 2021

OCA: Protected "7p6f" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7p6f is ON HOLD Authors: Description: Category: Unreleased Structures