7p6m - Revision history

OCA at 13:01, 1 February 2024

OCA — Thu, 01 Feb 2024 13:01:50 GMT

←Older revision		Revision as of 13:01, 1 February 2024
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	==Hydrogenated refolded hen egg-white lysozyme==		==Hydrogenated refolded hen egg-white lysozyme==
-	<StructureSection load='7p6m' size='340' side='right'caption='[[7p6m]]' scene=''>	+	<StructureSection load='7p6m' size='340' side='right'caption='[[7p6m]], [[Resolution\|resolution]] 0.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P6M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7p6m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P6M FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p6m OCA], [https://pdbe.org/7p6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p6m RCSB], [https://www.ebi.ac.uk/pdbsum/7p6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p6m ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 0.89Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p6m OCA], [https://pdbe.org/7p6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p6m RCSB], [https://www.ebi.ac.uk/pdbsum/7p6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p6m ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The biological function of a protein is intimately related to its structure and dynamics, which in turn are determined by the way in which it has been folded. In vitro refolding is commonly used for the recovery of recombinant proteins that are expressed in the form of inclusion bodies and is of central interest in terms of the folding pathways that occur in vivo. Here, biophysical data are reported for in vitro-refolded hydrogenated hen egg-white lysozyme, in combination with atomic resolution X-ray diffraction analyses, which allowed detailed comparisons with native hydrogenated and refolded perdeuterated lysozyme. Distinct folding modes are observed for the hydrogenated and perdeuterated refolded variants, which are determined by conformational changes to the backbone structure of the Lys97-Gly104 flexible loop. Surprisingly, the structure of the refolded perdeuterated protein is closer to that of native lysozyme than that of the refolded hydrogenated protein. These structural differences suggest that the observed decreases in thermal stability and enzymatic activity in the refolded perdeuterated and hydrogenated proteins are consequences of the macromolecular deuteration effect and of distinct folding dynamics, respectively. These results are discussed in the context of both in vitro and in vivo folding, as well as of lysozyme amyloidogenesis.
		+
		+	The impact of folding modes and deuteration on the atomic resolution structure of hen egg-white lysozyme.,Ramos J, Laux V, Haertlein M, Forsyth VT, Mossou E, Larsen S, Langkilde AE Acta Crystallogr D Struct Biol. 2021 Dec 1;77(Pt 12):1579-1590. doi:, 10.1107/S2059798321010950. Epub 2021 Nov 17. PMID:34866613<ref>PMID:34866613</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7p6m" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Forsyth VT]]		[[Category: Forsyth VT]]

OCA at 15:27, 22 December 2021

OCA — Wed, 22 Dec 2021 15:27:32 GMT

←Older revision		Revision as of 15:27, 22 December 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7p6m ~~is ON HOLD until Paper Publication~~	+	==Hydrogenated refolded hen egg-white lysozyme==
-		+	<StructureSection load='7p6m' size='340' side='right'caption='[[7p6m]]' scene=''>
-	~~Authors~~: ~~Ramos, J~~.~~, Laux, V~~.~~, Haertlein, M~~., ~~Forsyth, V~~.T., ~~Mossou, E~~., ~~Larsen~~, S., ~~Langkilde, A~~.E.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P6M FirstGlance]. <br>
-	~~Description: Hydrogenated refolded hen egg-white lysozyme~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p6m OCA], [https://pdbe.org/7p6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p6m RCSB], [https://www.ebi.ac.uk/pdbsum/7p6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p6m ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Larsen, S~~]]	+	__TOC__
-	[[Category: Haertlein, M]]	+	</StructureSection>
-	[[Category: ~~Ramos, J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Langkilde, A.E~~]]	+	[[Category: Forsyth VT]]
-	[[Category: Laux, V]]	+	[[Category: Haertlein M]]
-	[[Category: Mossou, E]]	+	[[Category: Langkilde AE]]
-	[[Category: ~~Forsyth, V.T~~]]	+	[[Category: Larsen S]]
		+	[[Category: Laux V]]
		+	[[Category: Mossou E]]
		+	[[Category: Ramos J]]

OCA at 05:03, 11 August 2021

OCA — Wed, 11 Aug 2021 05:03:37 GMT

←Older revision		Revision as of 05:03, 11 August 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7p6m is ON HOLD until ~~sometime in the future~~	+	The entry 7p6m is ON HOLD until Paper Publication

	Authors: Ramos, J., Laux, V., Haertlein, M., Forsyth, V.T., Mossou, E., Larsen, S., Langkilde, A.E.		Authors: Ramos, J., Laux, V., Haertlein, M., Forsyth, V.T., Mossou, E., Larsen, S., Langkilde, A.E.
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	Description: Hydrogenated refolded hen egg-white lysozyme		Description: Hydrogenated refolded hen egg-white lysozyme
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Forsyth~~, ~~V.T~~]]	+	[[Category: Larsen, S]]
	[[Category: Haertlein, M]]		[[Category: Haertlein, M]]
-	[[Category: Laux, V]]
-	[[Category: Mossou, E]]
	[[Category: Ramos, J]]		[[Category: Ramos, J]]
-	[[Category: Larsen, S]]
	[[Category: Langkilde, A.E]]		[[Category: Langkilde, A.E]]
		+	[[Category: Laux, V]]
		+	[[Category: Mossou, E]]
		+	[[Category: Forsyth, V.T]]

OCA: Protected "7p6m" [edit=sysop:move=sysop]

OCA — Wed, 28 Jul 2021 09:29:37 GMT

Protected "7p6m" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:29, 28 July 2021

OCA: New page: '''Unreleased structure''' The entry 7p6m is ON HOLD until sometime in the future Authors: Ramos, J., Laux, V., Haertlein, M., Forsyth, V.T., Mossou, E., Larsen, S., Langkilde, A.E. De...

OCA — Wed, 28 Jul 2021 09:29:36 GMT

New page: '''Unreleased structure''' The entry 7p6m is ON HOLD until sometime in the future Authors: Ramos, J., Laux, V., Haertlein, M., Forsyth, V.T., Mossou, E., Larsen, S., Langkilde, A.E. De...

New page

'''Unreleased structure'''

The entry 7p6m is ON HOLD until sometime in the future

Authors: Ramos, J., Laux, V., Haertlein, M., Forsyth, V.T., Mossou, E., Larsen, S., Langkilde, A.E.

Description: Hydrogenated refolded hen egg-white lysozyme
[[Category: Unreleased Structures]]
[[Category: Forsyth, V.T]]
[[Category: Haertlein, M]]
[[Category: Laux, V]]
[[Category: Mossou, E]]
[[Category: Ramos, J]]
[[Category: Larsen, S]]
[[Category: Langkilde, A.E]]