7p9w - Revision history

OCA at 13:03, 1 February 2024

OCA — Thu, 01 Feb 2024 13:03:00 GMT

←Older revision		Revision as of 13:03, 1 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7p9w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_herpesvirus_4_strain_B95-8 Human herpesvirus 4 strain B95-8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P9W FirstGlance]. <br>		<table><tr><td colspan='2'>[[7p9w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_herpesvirus_4_strain_B95-8 Human herpesvirus 4 strain B95-8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P9W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=PDO:1,3-PROPANDIOL'>PDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.0001006Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=PDO:1,3-PROPANDIOL'>PDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p9w OCA], [https://pdbe.org/7p9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p9w RCSB], [https://www.ebi.ac.uk/pdbsum/7p9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p9w ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p9w OCA], [https://pdbe.org/7p9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p9w RCSB], [https://www.ebi.ac.uk/pdbsum/7p9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p9w ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:23, 24 November 2022

OCA — Thu, 24 Nov 2022 10:23:31 GMT

←Older revision		Revision as of 10:23, 24 November 2022
Line 1:		Line 1:

-	====	+	==Epstein-Barr virus encoded apoptosis regulator BHRF1 in complex with Puma BH3==
-	<StructureSection load='7p9w' size='340' side='right'caption='[[7p9w]]' scene=''>	+	<StructureSection load='7p9w' size='340' side='right'caption='[[7p9w]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7p9w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_herpesvirus_4_strain_B95-8 Human herpesvirus 4 strain B95-8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P9W FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p9w OCA], [https://pdbe.org/7p9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p9w RCSB], [https://www.ebi.ac.uk/pdbsum/7p9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p9w ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=PDO:1,3-PROPANDIOL'>PDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p9w OCA], [https://pdbe.org/7p9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p9w RCSB], [https://www.ebi.ac.uk/pdbsum/7p9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p9w ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BBC3_HUMAN BBC3_HUMAN] Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.<ref>PMID:11463391</ref> <ref>PMID:23340338</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Apoptosis is a powerful defense mechanism used by multicellular organisms to counteract viral infection. In response to premature host cell suicide, viruses have evolved numerous countermeasures to ensure cell viability to optimize their replication by encoding proteins homologous in structure and function to cellular pro-survival Bcl-2 proteins. Epstein-Barr virus (EBV), a member of the Gammaherpesviridae, encodes the Bcl-2 homolog BHRF1, a potent inhibitor of Bcl-2-mediated apoptosis. BHRF1 acts by directly targeting Bid and Puma, two proapoptotic proteins of the Bcl-2 family. Here, we determined the crystal structures of BHRF1 bound to peptides spanning the Bcl-2 binding motifs (Bcl-2 homology 3 motif, BH3) of Bid and Puma. BHRF1 engages BH3 peptides using the canonical ligand-binding groove of its Bcl-2 fold and maintains a salt bridge between an Arg residue with a conserved Asp residue in the BH3 motif mimicking the canonical ionic interaction seen in host Bcl-2:BH3 motif complexes. Furthermore, both Bid and Puma utilize a fifth binding pocket in the canonical ligand binding groove of BHRF1 to provide an additional hydrophobic interaction distinct from the interactions previously seen with Bak and Bim. These findings provide a structural basis for EBV-mediated suppression of host cell apoptosis and reveal the flexibility of virus encoded Bcl-2 proteins in mimicking key interactions from the endogenous host signaling pathways.
		+
		+	Crystal Structures of Epstein-Barr Virus Bcl-2 Homolog BHRF1 Bound to Bid and Puma BH3 Motif Peptides.,Suraweera CD, Hinds MG, Kvansakul M Viruses. 2022 Oct 9;14(10):2222. doi: 10.3390/v14102222. PMID:36298777<ref>PMID:36298777</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7p9w" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Human herpesvirus 4 strain B95-8]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Hinds MG]]
		+	[[Category: Kvansakul M]]
		+	[[Category: Suraweera CD]]

OCA at 04:58, 10 August 2022

OCA — Wed, 10 Aug 2022 04:58:32 GMT

←Older revision		Revision as of 04:58, 10 August 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7p9w ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7p9w' size='340' side='right'caption='[[7p9w]]' scene=''>
-	~~Authors~~: ~~Suraweera, C~~.D.~~, Hinds, M~~.G.~~, Kvansakul, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Epstein-Barr virus encoded apoptosis regulator BHRF1 in complex with Puma BH3~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p9w OCA], [https://pdbe.org/7p9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p9w RCSB], [https://www.ebi.ac.uk/pdbsum/7p9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p9w ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Kvansakul~~, M]]	+	__TOC__
-	[[Category: ~~Suraweera, C.D~~]]	+	</StructureSection>
-	[[Category: ~~Hinds, M.G~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:27, 8 September 2021

OCA — Wed, 08 Sep 2021 07:27:18 GMT

←Older revision		Revision as of 07:27, 8 September 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7p9w is ON HOLD	+	The entry 7p9w is ON HOLD until Paper Publication

	Authors: Suraweera, C.D., Hinds, M.G., Kvansakul, M.		Authors: Suraweera, C.D., Hinds, M.G., Kvansakul, M.

OCA: Protected "7p9w" [edit=sysop:move=sysop]

OCA — Wed, 04 Aug 2021 09:15:45 GMT

Protected "7p9w" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:15, 4 August 2021

OCA: New page: '''Unreleased structure''' The entry 7p9w is ON HOLD Authors: Suraweera, C.D., Hinds, M.G., Kvansakul, M. Description: Epstein-Barr virus encoded apoptosis regulator BHRF1 in complex w...

OCA — Wed, 04 Aug 2021 09:15:44 GMT

New page: '''Unreleased structure''' The entry 7p9w is ON HOLD Authors: Suraweera, C.D., Hinds, M.G., Kvansakul, M. Description: Epstein-Barr virus encoded apoptosis regulator BHRF1 in complex w...

New page

'''Unreleased structure'''

The entry 7p9w is ON HOLD

Authors: Suraweera, C.D., Hinds, M.G., Kvansakul, M.

Description: Epstein-Barr virus encoded apoptosis regulator BHRF1 in complex with Puma BH3
[[Category: Unreleased Structures]]
[[Category: Kvansakul, M]]
[[Category: Suraweera, C.D]]
[[Category: Hinds, M.G]]