7pf3 - Revision history

OCA at 03:09, 8 September 2022

OCA — Thu, 08 Sep 2022 03:09:34 GMT

←Older revision		Revision as of 03:09, 8 September 2022
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-	====	+	==Nucleosome 4 of the 4x187 nucleosome array containing H1==
-	<StructureSection load='7pf3' size='340' side='right'caption='[[7pf3]]' scene=''>	+	<StructureSection load='7pf3' size='340' side='right'caption='[[7pf3]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pf3]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PF3 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pf3 OCA], [https://pdbe.org/7pf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pf3 RCSB], [https://www.ebi.ac.uk/pdbsum/7pf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pf3 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pf3 OCA], [https://pdbe.org/7pf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pf3 RCSB], [https://www.ebi.ac.uk/pdbsum/7pf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pf3 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/H32_HUMAN H32_HUMAN]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Throughout the genome, nucleosomes often form regular arrays that differ in nucleosome repeat length (NRL), occupancy of linker histone H1 and transcriptional activity. Here, we report cryo-EM structures of human H1-containing tetranucleosome arrays with four physiologically relevant NRLs. The structures show a zig-zag arrangement of nucleosomes, with nucleosomes 1 and 3 forming a stack. H1 binding to stacked nucleosomes depends on the NRL, whereas H1 always binds to the non-stacked nucleosomes 2 and 4. Short NRLs lead to altered trajectories of linker DNA, and these altered trajectories sterically impair H1 binding to the stacked nucleosomes in our structures. As the NRL increases, linker DNA trajectories relax, enabling H1 contacts and binding. Our results provide an explanation for why arrays with short NRLs are depleted of H1 and suited for transcription, whereas arrays with long NRLs show full H1 occupancy and can form transcriptionally silent heterochromatin regions.
		+
		+	Histone H1 binding to nucleosome arrays depends on linker DNA length and trajectory.,Dombrowski M, Engeholm M, Dienemann C, Dodonova S, Cramer P Nat Struct Mol Biol. 2022 May;29(5):493-501. doi: 10.1038/s41594-022-00768-w., Epub 2022 May 17. PMID:35581345<ref>PMID:35581345</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7pf3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Cramer P]]
		+	[[Category: Dombrowski M]]

OCA at 04:51, 3 August 2022

OCA — Wed, 03 Aug 2022 04:51:10 GMT

←Older revision		Revision as of 04:51, 3 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7pf3 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7pf3' size='340' side='right'caption='[[7pf3]]' scene=''>
-	~~Authors~~: ~~Dombrowski, M~~.~~, Cramer, P~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Nucleosome 4 of~~ the ~~4x187 nucleosome array containing H1~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pf3 OCA], [https://pdbe.org/7pf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pf3 RCSB], [https://www.ebi.ac.uk/pdbsum/7pf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pf3 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Dombrowski, M~~]]	+	__TOC__
-	[[Category: ~~Cramer, P~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:30, 8 September 2021

OCA — Wed, 08 Sep 2021 07:30:12 GMT

←Older revision		Revision as of 07:30, 8 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7pf3 is ON HOLD	+	The entry 7pf3 is ON HOLD until Paper Publication

	Authors: Dombrowski, M., Cramer, P.		Authors: Dombrowski, M., Cramer, P.

-	Description: 4x187 nucleosome 4	+	Description: Nucleosome 4 of the 4x187 nucleosome array containing H1
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Dombrowski, M]]		[[Category: Dombrowski, M]]
	[[Category: Cramer, P]]		[[Category: Cramer, P]]

OCA: Protected "7pf3" [edit=sysop:move=sysop]

OCA — Wed, 18 Aug 2021 05:03:21 GMT

Protected "7pf3" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:03, 18 August 2021

OCA: New page: '''Unreleased structure''' The entry 7pf3 is ON HOLD Authors: Dombrowski, M., Cramer, P. Description: 4x187 nucleosome 4 Category: Unreleased Structures Category: Dombrowski, M...

OCA — Wed, 18 Aug 2021 05:03:20 GMT

New page: '''Unreleased structure''' The entry 7pf3 is ON HOLD Authors: Dombrowski, M., Cramer, P. Description: 4x187 nucleosome 4 Category: Unreleased Structures Category: Dombrowski, M...

New page

'''Unreleased structure'''

The entry 7pf3 is ON HOLD

Authors: Dombrowski, M., Cramer, P.

Description: 4x187 nucleosome 4
[[Category: Unreleased Structures]]
[[Category: Dombrowski, M]]
[[Category: Cramer, P]]