OCA at 13:04, 1 February 2024

OCA — Thu, 01 Feb 2024 13:04:52 GMT

←Older revision		Revision as of 13:04, 1 February 2024
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	==20 minute Fe2+ soaked structure of SynFtn Variant D65A==		==20 minute Fe2+ soaked structure of SynFtn Variant D65A==
-	<StructureSection load='7pfi' size='340' side='right'caption='[[7pfi]]' scene=''>	+	<StructureSection load='7pfi' size='340' side='right'caption='[[7pfi]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PFI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pfi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_sp._CC9311 Synechococcus sp. CC9311]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PFI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pfi OCA], [https://pdbe.org/7pfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pfi RCSB], [https://www.ebi.ac.uk/pdbsum/7pfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pfi ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pfi OCA], [https://pdbe.org/7pfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pfi RCSB], [https://www.ebi.ac.uk/pdbsum/7pfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pfi ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q0I9X8_SYNS3 Q0I9X8_SYNS3] Iron-storage protein.[RuleBase:RU361145]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ferritins are proteins forming 24meric rhombic dodecahedral cages that play a key role in iron storage and detoxification in all cell types. Their function requires the transport of Fe(2+) from the exterior of the protein to buried di-iron catalytic sites, known as ferroxidase centres, where Fe(2+) is oxidized to form Fe(3+)-oxo precursors of the ferritin mineral core. The route of iron transit through animal ferritins is well understood: the Fe(2+) substrate enters the protein via channels at the threefold axes and conserved carboxylates on the inner surface of the protein cage have been shown to contribute to transient binding sites that guide Fe(2+) to the ferroxidase centres. The routes of iron transit through prokaryotic ferritins are less well studied but for some, at least, there is evidence that channels at the twofold axes are the major route for Fe(2+) uptake. SynFtn, isolated from the cyanobacterium Synechococcus CC9311, is an atypical prokaryotic ferritin that was recently shown to take up Fe(2+) via its threefold channels. However, the transfer site carboxylate residues conserved in animal ferritins are absent, meaning that the route taken from the site of iron entry into SynFtn to the catalytic centre is yet to be defined. Here, we report the use of a combination of site-directed mutagenesis, absorbance-monitored activity assays and protein crystallography to probe the effect of substitution of two residues potentially involved in this pathway. Both Glu141 and Asp65 play a role in guiding the Fe(2+) substrate to the ferroxidase centre. In the absence of Asp65, routes for Fe(2+) to, and Fe(3+) exit from, the ferroxidase centre are affected resulting in inefficient formation of the mineral core. These observations further define the iron transit route in what may be the first characterized example of a new class of ferritins peculiar to cyanobacteria.
		+
		+	Key carboxylate residues for iron transit through the prokaryotic ferritin SynFtn.,Bradley JM, Fair J, Hemmings AM, Le Brun NE Microbiology (Reading). 2021 Nov;167(11). doi: 10.1099/mic.0.001105. PMID:34825885<ref>PMID:34825885</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7pfi" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ferritin 3D structures\|Ferritin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Synechococcus sp. CC9311]]
	[[Category: Bradley JM]]		[[Category: Bradley JM]]
	[[Category: Hemmings AM]]		[[Category: Hemmings AM]]

OCA at 09:57, 8 December 2021

OCA — Wed, 08 Dec 2021 09:57:31 GMT

←Older revision		Revision as of 09:57, 8 December 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7pfi ~~is ON HOLD until Paper Publication~~	+	==20 minute Fe2+ soaked structure of SynFtn Variant D65A==
-		+	<StructureSection load='7pfi' size='340' side='right'caption='[[7pfi]]' scene=''>
-	~~Authors~~: ~~Hemmings~~, A.M., ~~Bradley~~, J.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PFI FirstGlance]. <br>
-	~~Description: 20 minute Fe2+ soaked structure of SynFtn Variant D65A~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pfi OCA], [https://pdbe.org/7pfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pfi RCSB], [https://www.ebi.ac.uk/pdbsum/7pfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pfi ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Bradley~~, J.M~~]]	+	__TOC__
-	[[Category: Hemmings~~, A.M~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bradley JM]]
		+	[[Category: Hemmings AM]]

OCA: Protected "7pfi" [edit=sysop:move=sysop]

OCA — Wed, 18 Aug 2021 05:03:52 GMT

Protected "7pfi" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:03, 18 August 2021

OCA: New page: '''Unreleased structure''' The entry 7pfi is ON HOLD until Paper Publication Authors: Hemmings, A.M., Bradley, J.M. Description: 20 minute Fe2+ soaked structure of SynFtn Variant D65A ...

OCA — Wed, 18 Aug 2021 05:03:51 GMT

New page: '''Unreleased structure''' The entry 7pfi is ON HOLD until Paper Publication Authors: Hemmings, A.M., Bradley, J.M. Description: 20 minute Fe2+ soaked structure of SynFtn Variant D65A ...

New page

'''Unreleased structure'''

The entry 7pfi is ON HOLD until Paper Publication

Authors: Hemmings, A.M., Bradley, J.M.

Description: 20 minute Fe2+ soaked structure of SynFtn Variant D65A
[[Category: Unreleased Structures]]
[[Category: Bradley, J.M]]
[[Category: Hemmings, A.M]]

7pfi - Revision history

OCA at 13:04, 1 February 2024

OCA at 09:57, 8 December 2021

OCA: Protected "7pfi" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7pfi is ON HOLD until Paper Publication Authors: Hemmings, A.M., Bradley, J.M. Description: 20 minute Fe2+ soaked structure of SynFtn Variant D65A ...