OCA at 16:26, 18 October 2023

OCA — Wed, 18 Oct 2023 16:26:29 GMT

←Older revision		Revision as of 16:26, 18 October 2023
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-	====	+	==Core human replisome==
-	<StructureSection load='7pfo' size='340' side='right'caption='[[7pfo]]' scene=''>	+	<StructureSection load='7pfo' size='340' side='right'caption='[[7pfo]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pfo]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PFO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PFO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pfo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pfo OCA], [https://pdbe.org/7pfo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pfo RCSB], [https://www.ebi.ac.uk/pdbsum/7pfo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pfo ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pfo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pfo OCA], [https://pdbe.org/7pfo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pfo RCSB], [https://www.ebi.ac.uk/pdbsum/7pfo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pfo ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MCM6_HUMAN MCM6_HUMAN] Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.<ref>PMID:9305914</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The human replisome is an elaborate arrangement of molecular machines responsible for accurate chromosome replication. At its heart is the CDC45-MCM-GINS (CMG) helicase, which, in addition to unwinding the parental DNA duplex, arranges many proteins including the leading-strand polymerase Pol epsilon, together with TIMELESS-TIPIN, CLASPIN and AND-1 that have key and varied roles in maintaining smooth replisome progression. How these proteins are coordinated in the human replisome is poorly understood. We have determined a 3.2 A cryo-EM structure of a human replisome comprising CMG, Pol epsilon, TIMELESS-TIPIN, CLASPIN and AND-1 bound to replication fork DNA. The structure permits a detailed understanding of how AND-1, TIMELESS-TIPIN and Pol epsilon engage CMG, reveals how CLASPIN binds to multiple replisome components and identifies the position of the Pol epsilon catalytic domain. Furthermore, the intricate network of contacts contributed by MCM subunits and TIMELESS-TIPIN with replication fork DNA suggests a mechanism for strand separation.
		+
		+	Structure of a human replisome shows the organisation and interactions of a DNA replication machine.,Jones ML, Baris Y, Taylor MRG, Yeeles JTP EMBO J. 2021 Dec 1;40(23):e108819. doi: 10.15252/embj.2021108819. Epub 2021 Oct , 25. PMID:34694004<ref>PMID:34694004</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7pfo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Jones MJ]]
		+	[[Category: Yeeles JTP]]

OCA at 06:16, 10 November 2021

OCA — Wed, 10 Nov 2021 06:16:29 GMT

←Older revision		Revision as of 06:16, 10 November 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7pfo ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7pfo' size='340' side='right'caption='[[7pfo]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pfo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pfo OCA], [https://pdbe.org/7pfo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pfo RCSB], [https://www.ebi.ac.uk/pdbsum/7pfo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pfo ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7pfo" [edit=sysop:move=sysop]

OCA — Wed, 18 Aug 2021 05:04:12 GMT

Protected "7pfo" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:04, 18 August 2021

OCA: New page: '''Unreleased structure''' The entry 7pfo is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 18 Aug 2021 05:04:12 GMT

New page: '''Unreleased structure''' The entry 7pfo is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7pfo is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7pfo - Revision history

OCA at 16:26, 18 October 2023

OCA at 06:16, 10 November 2021

OCA: Protected "7pfo" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7pfo is ON HOLD Authors: Description: Category: Unreleased Structures