7pgy - Revision history

OCA at 13:05, 1 February 2024

OCA — Thu, 01 Feb 2024 13:05:19 GMT

←Older revision		Revision as of 13:05, 1 February 2024
Line 1:		Line 1:

	==Structure of light-adapted AsLOV2 wild type==		==Structure of light-adapted AsLOV2 wild type==
-	<StructureSection load='7pgy' size='340' side='right'caption='[[7pgy]]' scene=''>	+	<StructureSection load='7pgy' size='340' side='right'caption='[[7pgy]], [[Resolution\|resolution]] 1.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pgy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Avena_sativa Avena sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgy OCA], [https://pdbe.org/7pgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgy RCSB], [https://www.ebi.ac.uk/pdbsum/7pgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.09Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgy OCA], [https://pdbe.org/7pgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgy RCSB], [https://www.ebi.ac.uk/pdbsum/7pgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O49003_AVESA O49003_AVESA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In nature as in biotechnology, light-oxygen-voltage photoreceptors perceive blue light to elicit spatiotemporally defined cellular responses. Photon absorption drives thioadduct formation between a conserved cysteine and the flavin chromophore. An equally conserved, proximal glutamine processes the resultant flavin protonation into downstream hydrogen-bond rearrangements. Here, we report that this glutamine, long deemed essential, is generally dispensable. In its absence, several light-oxygen-voltage receptors invariably retained productive, if often attenuated, signaling responses. Structures of a light-oxygen-voltage paradigm at around 1 A resolution revealed highly similar light-induced conformational changes, irrespective of whether the glutamine is present. Naturally occurring, glutamine-deficient light-oxygen-voltage receptors likely serve as bona fide photoreceptors, as we showcase for a diguanylate cyclase. We propose that without the glutamine, water molecules transiently approach the chromophore and thus propagate flavin protonation downstream. Signaling without glutamine appears intrinsic to light-oxygen-voltage receptors, which pertains to biotechnological applications and suggests evolutionary descendance from redox-active flavoproteins.
		+
		+	Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine.,Dietler J, Gelfert R, Kaiser J, Borin V, Renzl C, Pilsl S, Ranzani AT, Garcia de Fuentes A, Gleichmann T, Diensthuber RP, Weyand M, Mayer G, Schapiro I, Moglich A Nat Commun. 2022 May 12;13(1):2618. doi: 10.1038/s41467-022-30252-4. PMID:35552382<ref>PMID:35552382</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7pgy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Avena sativa]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Gelfert R]]		[[Category: Gelfert R]]
	[[Category: Moeglich A]]		[[Category: Moeglich A]]
	[[Category: Weyand M]]		[[Category: Weyand M]]

OCA at 14:34, 31 August 2022

OCA — Wed, 31 Aug 2022 14:34:01 GMT

←Older revision		Revision as of 14:34, 31 August 2022
Line 1:		Line 1:

	==Structure of light-adapted AsLOV2 wild type==		==Structure of light-adapted AsLOV2 wild type==
-	<StructureSection load='7pgy' size='340' side='right'caption='[[7pgy]]~~, [[Resolution\|resolution]] 1.09Å~~' scene=''>	+	<StructureSection load='7pgy' size='340' side='right'caption='[[7pgy]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[7pgy]] is a 1 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGY FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgy OCA], [https://pdbe.org/7pgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgy RCSB], [https://www.ebi.ac.uk/pdbsum/7pgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgy ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgy OCA], [https://pdbe.org/7pgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgy RCSB], [https://www.ebi.ac.uk/pdbsum/7pgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgy ProSAT]</span></td></tr>	+
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In nature as in biotechnology, light-oxygen-voltage photoreceptors perceive blue light to elicit spatiotemporally defined cellular responses. Photon absorption drives thioadduct formation between a conserved cysteine and the flavin chromophore. An equally conserved, proximal glutamine processes the resultant flavin protonation into downstream hydrogen-bond rearrangements. Here, we report that this glutamine, long deemed essential, is generally dispensable. In its absence, several light-oxygen-voltage receptors invariably retained productive, if often attenuated, signaling responses. Structures of a light-oxygen-voltage paradigm at around 1 A resolution revealed highly similar light-induced conformational changes, irrespective of whether the glutamine is present. Naturally occurring, glutamine-deficient light-oxygen-voltage receptors likely serve as bona fide photoreceptors, as we showcase for a diguanylate cyclase. We propose that without the glutamine, water molecules transiently approach the chromophore and thus propagate flavin protonation downstream. Signaling without glutamine appears intrinsic to light-oxygen-voltage receptors, which pertains to biotechnological applications and suggests evolutionary descendance from redox-active flavoproteins.
-
-	Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine.,Dietler J, Gelfert R, Kaiser J, Borin V, Renzl C, Pilsl S, Ranzani AT, Garcia de Fuentes A, Gleichmann T, Diensthuber RP, Weyand M, Mayer G, Schapiro I, Moglich A Nat Commun. 2022 May 12;13(1):2618. doi: 10.1038/s41467-022-30252-4. PMID:35552382<ref>PMID:35552382</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 7pgy" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gelfert, R]]	+	[[Category: Gelfert R]]
-	[[Category: Moeglich, A]]	+	[[Category: Moeglich A]]
-	[[Category: Weyand, M]]	+	[[Category: Weyand M]]
-	~~[[Category: Flavin]]~~	+
-	~~[[Category: Light-oxygen-voltage]]~~	+
-	~~[[Category: Optogenetic]]~~	+
-	~~[[Category: Photoreceptor]]~~	+
-	~~[[Category: Phototropin]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 08:13, 25 May 2022

OCA — Wed, 25 May 2022 08:13:11 GMT

←Older revision		Revision as of 08:13, 25 May 2022
Line 1:		Line 1:

	==Structure of light-adapted AsLOV2 wild type==		==Structure of light-adapted AsLOV2 wild type==
-	<StructureSection load='7pgy' size='340' side='right'caption='[[7pgy]]' scene=''>	+	<StructureSection load='7pgy' size='340' side='right'caption='[[7pgy]], [[Resolution\|resolution]] 1.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pgy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgy OCA], [https://pdbe.org/7pgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgy RCSB], [https://www.ebi.ac.uk/pdbsum/7pgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgy ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgy OCA], [https://pdbe.org/7pgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgy RCSB], [https://www.ebi.ac.uk/pdbsum/7pgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgy ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In nature as in biotechnology, light-oxygen-voltage photoreceptors perceive blue light to elicit spatiotemporally defined cellular responses. Photon absorption drives thioadduct formation between a conserved cysteine and the flavin chromophore. An equally conserved, proximal glutamine processes the resultant flavin protonation into downstream hydrogen-bond rearrangements. Here, we report that this glutamine, long deemed essential, is generally dispensable. In its absence, several light-oxygen-voltage receptors invariably retained productive, if often attenuated, signaling responses. Structures of a light-oxygen-voltage paradigm at around 1 A resolution revealed highly similar light-induced conformational changes, irrespective of whether the glutamine is present. Naturally occurring, glutamine-deficient light-oxygen-voltage receptors likely serve as bona fide photoreceptors, as we showcase for a diguanylate cyclase. We propose that without the glutamine, water molecules transiently approach the chromophore and thus propagate flavin protonation downstream. Signaling without glutamine appears intrinsic to light-oxygen-voltage receptors, which pertains to biotechnological applications and suggests evolutionary descendance from redox-active flavoproteins.
		+
		+	Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine.,Dietler J, Gelfert R, Kaiser J, Borin V, Renzl C, Pilsl S, Ranzani AT, Garcia de Fuentes A, Gleichmann T, Diensthuber RP, Weyand M, Mayer G, Schapiro I, Moglich A Nat Commun. 2022 May 12;13(1):2618. doi: 10.1038/s41467-022-30252-4. PMID:35552382<ref>PMID:35552382</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7pgy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gelfert R]]	+	[[Category: Gelfert, R]]
-	[[Category: Moeglich A]]	+	[[Category: Moeglich, A]]
-	[[Category: Weyand M]]	+	[[Category: Weyand, M]]
		+	[[Category: Flavin]]
		+	[[Category: Light-oxygen-voltage]]
		+	[[Category: Optogenetic]]
		+	[[Category: Photoreceptor]]
		+	[[Category: Phototropin]]
		+	[[Category: Signaling protein]]

OCA at 06:18, 12 May 2022

OCA — Thu, 12 May 2022 06:18:45 GMT

←Older revision		Revision as of 06:18, 12 May 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7pgy is ~~ON HOLD until Paper Publication~~	+	==Structure of light-adapted AsLOV2 wild type==
-		+	<StructureSection load='7pgy' size='340' side='right'caption='[[7pgy]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGY FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgy OCA], [https://pdbe.org/7pgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgy RCSB], [https://www.ebi.ac.uk/pdbsum/7pgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgy ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Gelfert R]]
		+	[[Category: Moeglich A]]
		+	[[Category: Weyand M]]

OCA: Protected "7pgy" [edit=sysop:move=sysop]

OCA — Wed, 25 Aug 2021 05:11:52 GMT

Protected "7pgy" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:11, 25 August 2021

OCA: New page: '''Unreleased structure''' The entry 7pgy is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 25 Aug 2021 05:11:50 GMT

New page: '''Unreleased structure''' The entry 7pgy is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7pgy is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]