OCA at 05:09, 10 August 2022

OCA — Wed, 10 Aug 2022 05:09:11 GMT

←Older revision		Revision as of 05:09, 10 August 2022
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	==Human voltage-gated potassium channel Kv3.1 (apo condition)==		==Human voltage-gated potassium channel Kv3.1 (apo condition)==
-	<StructureSection load='7phh' size='340' side='right'caption='[[7phh]]' scene=''>	+	<StructureSection load='7phh' size='340' side='right'caption='[[7phh]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PHH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7phh]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PHH FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7phh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7phh OCA], [https://pdbe.org/7phh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7phh RCSB], [https://www.ebi.ac.uk/pdbsum/7phh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7phh ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PCF:1,2-DIACYL-SN-GLYCERO-3-PHOSHOCHOLINE'>PCF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7phh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7phh OCA], [https://pdbe.org/7phh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7phh RCSB], [https://www.ebi.ac.uk/pdbsum/7phh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7phh ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Kv3 channels have distinctive gating kinetics tailored for rapid repolarization in fast-spiking neurons. Malfunction of this process due to genetic variants in the KCNC1 gene causes severe epileptic disorders, yet the structural determinants for the unusual gating properties remain elusive. Here, we present cryo-electron microscopy structures of the human Kv3.1a channel, revealing a unique arrangement of the cytoplasmic tetramerization domain T1 which facilitates interactions with C-terminal axonal targeting motif and key components of the gating machinery. Additional interactions between S1/S2 linker and turret domain strengthen the interface between voltage sensor and pore domain. Supported by molecular dynamics simulations, electrophysiological and mutational analyses, we identify several residues in the S4/S5 linker which influence the gating kinetics and an electrostatic interaction between acidic residues in alpha6 of T1 and R449 in the pore-flanking S6T helices. These findings provide insights into gating control and disease mechanisms and may guide strategies for the design of pharmaceutical drugs targeting Kv3 channels.
		+
		+	Cryo-EM structure of the human Kv3.1 channel reveals gating control by the cytoplasmic T1 domain.,Chi G, Liang Q, Sridhar A, Cowgill JB, Sader K, Radjainia M, Qian P, Castro-Hartmann P, Venkaya S, Singh NK, McKinley G, Fernandez-Cid A, Mukhopadhyay SMM, Burgess-Brown NA, Delemotte L, Covarrubias M, Durr KL Nat Commun. 2022 Jul 15;13(1):4087. doi: 10.1038/s41467-022-29594-w. PMID:35840580<ref>PMID:35840580</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7phh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Burgess-Brown NA]]	+	[[Category: Burgess-Brown, N A]]
-	[[Category: Chi G]]	+	[[Category: Chi, G]]
-	[[Category: Duerr KL]]	+	[[Category: Duerr, K L]]
-	[[Category: Fernandez-Cid A]]	+	[[Category: Fernandez-Cid, A]]
-	[[Category: MacLean EM]]	+	[[Category: MacLean, E M]]
-	[[Category: Marsden B]]	+	[[Category: Marsden, B]]
-	[[Category: McKinley G]]	+	[[Category: McKinley, G]]
-	[[Category: Mukhopadhyay ~~SMM~~]]	+	[[Category: Mukhopadhyay, S M.M]]
-	[[Category: Pike ~~ACW~~]]	+	[[Category: Pike, A C.W]]
-	[[Category: Ragan TJ]]	+	[[Category: Ragan, T J]]
-	[[Category: Savva C]]	+	[[Category: Savva, C]]
-	[[Category: Singh NK]]	+	[[Category: Singh, N K]]
-	[[Category: Venkaya S]]	+	[[Category: Venkaya, S]]
		+	[[Category: Channel]]
		+	[[Category: Membrane protein]]
		+	[[Category: Potassium channel]]
		+	[[Category: Tetramer]]
		+	[[Category: Transport protein]]
		+	[[Category: Voltage-gated]]

OCA at 06:19, 2 March 2022

OCA — Wed, 02 Mar 2022 06:19:41 GMT

←Older revision		Revision as of 06:19, 2 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7phh is ~~ON HOLD until Paper Publication~~	+	==Human voltage-gated potassium channel Kv3.1 (apo condition)==
-		+	<StructureSection load='7phh' size='340' side='right'caption='[[7phh]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PHH FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7phh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7phh OCA], [https://pdbe.org/7phh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7phh RCSB], [https://www.ebi.ac.uk/pdbsum/7phh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7phh ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Burgess-Brown NA]]
		+	[[Category: Chi G]]
		+	[[Category: Duerr KL]]
		+	[[Category: Fernandez-Cid A]]
		+	[[Category: MacLean EM]]
		+	[[Category: Marsden B]]
		+	[[Category: McKinley G]]
		+	[[Category: Mukhopadhyay SMM]]
		+	[[Category: Pike ACW]]
		+	[[Category: Ragan TJ]]
		+	[[Category: Savva C]]
		+	[[Category: Singh NK]]
		+	[[Category: Venkaya S]]

OCA at 08:09, 15 September 2021

OCA — Wed, 15 Sep 2021 08:09:35 GMT

←Older revision		Revision as of 08:09, 15 September 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7phh is ON HOLD	+	The entry 7phh is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7phh" [edit=sysop:move=sysop]

OCA — Wed, 25 Aug 2021 05:12:40 GMT

Protected "7phh" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:12, 25 August 2021

OCA: New page: '''Unreleased structure''' The entry 7phh is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 25 Aug 2021 05:12:39 GMT

New page: '''Unreleased structure''' The entry 7phh is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7phh is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7phh - Revision history

OCA at 05:09, 10 August 2022

OCA at 06:19, 2 March 2022

OCA at 08:09, 15 September 2021

OCA: Protected "7phh" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7phh is ON HOLD Authors: Description: Category: Unreleased Structures