7pjj - Revision history

OCA at 13:05, 1 February 2024

OCA — Thu, 01 Feb 2024 13:05:56 GMT

←Older revision		Revision as of 13:05, 1 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7pjj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_scabiei_87.22 Streptomyces scabiei 87.22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PJJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[7pjj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_scabiei_87.22 Streptomyces scabiei 87.22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PJJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.086Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pjj OCA], [https://pdbe.org/7pjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pjj RCSB], [https://www.ebi.ac.uk/pdbsum/7pjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pjj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pjj OCA], [https://pdbe.org/7pjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pjj RCSB], [https://www.ebi.ac.uk/pdbsum/7pjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pjj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/C9ZDY4_STRSW C9ZDY4_STRSW]]	+	[https://www.uniprot.org/uniprot/C9ZDY4_STRSW C9ZDY4_STRSW]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 7pjj" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7pjj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:46, 7 September 2022

OCA — Wed, 07 Sep 2022 19:46:21 GMT

←Older revision		Revision as of 19:46, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='7pjj' size='340' side='right'caption='[[7pjj]], [[Resolution\|resolution]] 3.09Å' scene=''>		<StructureSection load='7pjj' size='340' side='right'caption='[[7pjj]], [[Resolution\|resolution]] 3.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7pjj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PJJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pjj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_scabiei_87.22 Streptomyces scabiei 87.22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PJJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pjj OCA], [https://pdbe.org/7pjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pjj RCSB], [https://www.ebi.ac.uk/pdbsum/7pjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pjj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pjj OCA], [https://pdbe.org/7pjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pjj RCSB], [https://www.ebi.ac.uk/pdbsum/7pjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pjj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/C9ZDY4_STRSW C9ZDY4_STRSW]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beta-glucosidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Deflandre, B]]	+	[[Category: Streptomyces scabiei 87 22]]
-	[[Category: Herman, R]]	+	[[Category: Deflandre B]]
-	[[Category: Jadot, C]]	+	[[Category: Herman R]]
-	[[Category: Kerff, F]]	+	[[Category: Jadot C]]
-	[[Category: Rigali, S]]	+	[[Category: Kerff F]]
-	~~[[Category: Genetic compensation]]~~	+	[[Category: Rigali S]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: O-heteroside hydrolase]]~~	+
-	~~[[Category: Versatile beta-glucosidase~~]]	+

OCA at 06:43, 31 August 2022

OCA — Wed, 31 Aug 2022 06:43:18 GMT

←Older revision		Revision as of 06:43, 31 August 2022
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	==Structure of the Family-3 Glycosyl Hydrolase BcpE2 from Streptomyces scabies==		==Structure of the Family-3 Glycosyl Hydrolase BcpE2 from Streptomyces scabies==
-	<StructureSection load='7pjj' size='340' side='right'caption='[[7pjj]]' scene=''>	+	<StructureSection load='7pjj' size='340' side='right'caption='[[7pjj]], [[Resolution\|resolution]] 3.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PJJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pjj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PJJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pjj OCA], [https://pdbe.org/7pjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pjj RCSB], [https://www.ebi.ac.uk/pdbsum/7pjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pjj ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pjj OCA], [https://pdbe.org/7pjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pjj RCSB], [https://www.ebi.ac.uk/pdbsum/7pjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pjj ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cellulose being the most abundant polysaccharide on earth, beta-glucosidases hydrolyzing cello-oligosaccharides are key enzymes to fuel glycolysis in microorganisms developing on plant material. In Streptomyces scabiei, the causative agent of common scab in root and tuber crops, a genetic compensation phenomenon safeguards the loss of the gene encoding the cello-oligosaccharide hydrolase BglC by awakening the expression of alternative beta-glucosidases. Here, we revealed that the BglC compensating enzyme BcpE2 was the GH3-family beta-glucosidase that displayed the highest reported substrate promiscuity and was able to release the glucose moiety of all tested types of plant-derived heterosides (aryl beta-glucosides, monolignol glucosides, cyanogenic glucosides, anthocyanosides, and coumarin heterosides). BcpE2 structure analysis highlighted a large cavity in the PA14 domain that covered the active site, and the high flexibility of this domain would allow proper adjustment of this cavity for disparate heterosides. The exceptional substrate promiscuity of BcpE2 provides microorganisms a versatile tool for scavenging glucose from plant-derived nutrients that widely vary in size and structure. Importantly, scopolin was the only substrate commonly hydrolyzed by both BglC and BcpE2, thereby generating the potent virulence inhibitor scopoletin. Next to fueling glycolysis, both enzymes would also fine-tune the strength of virulence. IMPORTANCE Plant decaying biomass is the most abundant provider of carbon sources for soil-dwelling microorganisms. To optimally evolve in such environmental niches, microorganisms possess an arsenal of hydrolytic enzymatic complexes to feed on the various types of polysaccharides, oligosaccharides, and monosaccharides. In this work, structural, enzymatic, and expression studies revealed the existence of a "swiss-army knife" enzyme, BcpE2, that was able to retrieve the glucose moiety of a multitude of plant-derived substrates that vary in size, structure, and origin. This enzyme would provide the microorganisms with a tool that would allow them to find nutrients from any type of plant-derived material.
		+
		+	Structure and Function of BcpE2, the Most Promiscuous GH3-Family Glucose Scavenging Beta-Glucosidase.,Deflandre B, Jadot C, Planckaert S, Thiebaut N, Stulanovic N, Herman R, Devreese B, Kerff F, Rigali S mBio. 2022 Aug 1:e0093522. doi: 10.1128/mbio.00935-22. PMID:35913158<ref>PMID:35913158</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7pjj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Beta-glucosidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Deflandre B]]	+	[[Category: Deflandre, B]]
-	[[Category: Herman R]]	+	[[Category: Herman, R]]
-	[[Category: Jadot C]]	+	[[Category: Jadot, C]]
-	[[Category: Kerff F]]	+	[[Category: Kerff, F]]
-	[[Category: Rigali S]]	+	[[Category: Rigali, S]]
		+	[[Category: Genetic compensation]]
		+	[[Category: Hydrolase]]
		+	[[Category: O-heteroside hydrolase]]
		+	[[Category: Versatile beta-glucosidase]]

OCA at 05:15, 13 July 2022

OCA — Wed, 13 Jul 2022 05:15:01 GMT

←Older revision		Revision as of 05:15, 13 July 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7pjj is ~~ON HOLD until Paper Publication~~	+	==Structure of the Family-3 Glycosyl Hydrolase BcpE2 from Streptomyces scabies==
-		+	<StructureSection load='7pjj' size='340' side='right'caption='[[7pjj]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PJJ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pjj OCA], [https://pdbe.org/7pjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pjj RCSB], [https://www.ebi.ac.uk/pdbsum/7pjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pjj ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Deflandre B]]
		+	[[Category: Herman R]]
		+	[[Category: Jadot C]]
		+	[[Category: Kerff F]]
		+	[[Category: Rigali S]]

OCA: Protected "7pjj" [edit=sysop:move=sysop]

OCA — Wed, 08 Sep 2021 07:33:18 GMT

Protected "7pjj" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:33, 8 September 2021

OCA: New page: '''Unreleased structure''' The entry 7pjj is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 08 Sep 2021 07:33:17 GMT

New page: '''Unreleased structure''' The entry 7pjj is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7pjj is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]