OCA at 19:31, 19 October 2022

OCA — Wed, 19 Oct 2022 19:31:54 GMT

←Older revision		Revision as of 19:31, 19 October 2022
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	==Cryo-EM structures of human fucosidase FucA1 reveal insight into substate recognition and catalysis.==		==Cryo-EM structures of human fucosidase FucA1 reveal insight into substate recognition and catalysis.==
-	<StructureSection load='7pls' size='340' side='right'caption='[[7pls]]' scene=''>	+	<StructureSection load='7pls' size='340' side='right'caption='[[7pls]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PLS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pls]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PLS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pls OCA], [https://pdbe.org/7pls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pls RCSB], [https://www.ebi.ac.uk/pdbsum/7pls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pls ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pls OCA], [https://pdbe.org/7pls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pls RCSB], [https://www.ebi.ac.uk/pdbsum/7pls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pls ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/FUCO_HUMAN FUCO_HUMAN] Fucosidosis. The disease is caused by variants affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FUCO_HUMAN FUCO_HUMAN] Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.<ref>PMID:9741689</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enzymatic hydrolysis of alpha-L-fucose from fucosylated glycoconjugates is consequential in bacterial infections and the neurodegenerative lysosomal storage disorder fucosidosis. Understanding human alpha-L-fucosidase catalysis, in an effort toward drug design, has been hindered by the absence of three-dimensional structural data for any animal fucosidase. Here, we have used cryoelectron microscopy (cryo-EM) to determine the structure of human lysosomal alpha-L-fucosidase (FucA1) in both an unliganded state and in complex with the inhibitor deoxyfuconojirimycin. These structures, determined at 2.49 A resolution, reveal the homotetrameric structure of FucA1, the architecture of the catalytic center, and the location of both natural population variations and disease-causing mutations. Furthermore, this work has conclusively identified the hitherto contentious identity of the catalytic acid/base as aspartate-276, representing a shift from both the canonical glutamate acid/base residue and a previously proposed glutamate residue. These findings have furthered our understanding of how FucA1 functions in both health and disease.
		+
		+	Cryo-EM structures of human fucosidase FucA1 reveal insight into substrate recognition and catalysis.,Armstrong Z, Meek RW, Wu L, Blaza JN, Davies GJ Structure. 2022 Jul 19. pii: S0969-2126(22)00270-2. doi:, 10.1016/j.str.2022.07.001. PMID:35907402<ref>PMID:35907402</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7pls" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Armstrong Z]]		[[Category: Armstrong Z]]

OCA at 04:59, 10 August 2022

OCA — Wed, 10 Aug 2022 04:59:09 GMT

←Older revision		Revision as of 04:59, 10 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7pls is ~~ON HOLD until Paper Publication~~	+	==Cryo-EM structures of human fucosidase FucA1 reveal insight into substate recognition and catalysis.==
-		+	<StructureSection load='7pls' size='340' side='right'caption='[[7pls]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PLS FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pls OCA], [https://pdbe.org/7pls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pls RCSB], [https://www.ebi.ac.uk/pdbsum/7pls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pls ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Armstrong Z]]
		+	[[Category: Blaza JN]]
		+	[[Category: Davies GJ]]
		+	[[Category: Meek RW]]
		+	[[Category: Wu L]]

OCA at 04:04, 6 October 2021

OCA — Wed, 06 Oct 2021 04:04:19 GMT

←Older revision		Revision as of 04:04, 6 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7pls is ON HOLD	+	The entry 7pls is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7pls" [edit=sysop:move=sysop]

OCA — Wed, 08 Sep 2021 07:37:08 GMT

Protected "7pls" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:37, 8 September 2021

OCA: New page: '''Unreleased structure''' The entry 7pls is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 08 Sep 2021 07:37:07 GMT

New page: '''Unreleased structure''' The entry 7pls is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7pls is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7pls - Revision history

OCA at 19:31, 19 October 2022

OCA at 04:59, 10 August 2022

OCA at 04:04, 6 October 2021

OCA: Protected "7pls" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7pls is ON HOLD Authors: Description: Category: Unreleased Structures