OCA at 11:05, 15 November 2023

OCA — Wed, 15 Nov 2023 11:05:47 GMT

←Older revision		Revision as of 11:05, 15 November 2023
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-	====	+	==Human mitochondrial ribosome small subunit==
-	<StructureSection load='7po3' size='340' side='right'caption='[[7po3]]' scene=''>	+	<StructureSection load='7po3' size='340' side='right'caption='[[7po3]], [[Resolution\|resolution]] 2.92Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7po3]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PO3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PO3 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7po3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7po3 OCA], [https://pdbe.org/7po3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7po3 RCSB], [https://www.ebi.ac.uk/pdbsum/7po3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7po3 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.92Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5F0:(3~{S})-3-azanyl-4-methoxy-4-oxidanylidene-butanoic+acid'>5F0</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=AYA:N-ACETYLALANINE'>AYA</scene>, <scene name='pdbligand=B8T:4-methyl,+cytidine-5-monophosphate'>B8T</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MA6:6N-DIMETHYLADENOSINE-5-MONOPHOSHATE'>MA6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7po3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7po3 OCA], [https://pdbe.org/7po3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7po3 RCSB], [https://www.ebi.ac.uk/pdbsum/7po3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7po3 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RT24_HUMAN RT24_HUMAN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mitoribosomes are essential for the synthesis and maintenance of bioenergetic proteins. Here we use cryo-electron microscopy to determine a series of the small mitoribosomal subunit (SSU) intermediates in complex with auxiliary factors, revealing a sequential assembly mechanism. The methyltransferase TFB1M binds to partially unfolded rRNA h45 that is promoted by RBFA, while the mRNA channel is blocked. This enables binding of METTL15 that promotes further rRNA maturation and a large conformational change of RBFA. The new conformation allows initiation factor mtIF3 to already occupy the subunit interface during the assembly. Finally, the mitochondria-specific ribosomal protein mS37 (ref. (1)) outcompetes RBFA to complete the assembly with the SSU-mS37-mtIF3 complex(2) that proceeds towards mtIF2 binding and translation initiation. Our results explain how the action of step-specific factors modulate the dynamic assembly of the SSU, and adaptation of a unique protein, mS37, links the assembly to initiation to establish the catalytic human mitoribosome.
		+
		+	Mechanism of mitoribosomal small subunit biogenesis and preinitiation.,Itoh Y, Khawaja A, Laptev I, Cipullo M, Atanassov I, Sergiev P, Rorbach J, Amunts A Nature. 2022 Jun;606(7914):603-608. doi: 10.1038/s41586-022-04795-x. Epub 2022 , Jun 8. PMID:35676484<ref>PMID:35676484</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7po3" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ribosome 3D structures\|Ribosome 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Amunts A]]
		+	[[Category: Itoh Y]]
		+	[[Category: Khawaja A]]
		+	[[Category: Rorbach J]]

OCA at 05:10, 15 June 2022

OCA — Wed, 15 Jun 2022 05:10:29 GMT

←Older revision		Revision as of 05:10, 15 June 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7po3 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7po3' size='340' side='right'caption='[[7po3]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7po3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7po3 OCA], [https://pdbe.org/7po3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7po3 RCSB], [https://www.ebi.ac.uk/pdbsum/7po3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7po3 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 10:12, 13 October 2021

OCA — Wed, 13 Oct 2021 10:12:58 GMT

←Older revision		Revision as of 10:12, 13 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7po3 is ON HOLD	+	The entry 7po3 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7po3" [edit=sysop:move=sysop]

OCA — Wed, 15 Sep 2021 08:12:54 GMT

Protected "7po3" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:12, 15 September 2021

OCA: New page: '''Unreleased structure''' The entry 7po3 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 15 Sep 2021 08:12:53 GMT

New page: '''Unreleased structure''' The entry 7po3 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7po3 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7po3 - Revision history

OCA at 11:05, 15 November 2023

OCA at 05:10, 15 June 2022

OCA at 10:12, 13 October 2021

OCA: Protected "7po3" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7po3 is ON HOLD Authors: Description: Category: Unreleased Structures