OCA at 03:07, 21 April 2022

OCA — Thu, 21 Apr 2022 03:07:30 GMT

←Older revision		Revision as of 03:07, 21 April 2022
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	==Crystal structure of the CH domain of human CEP44==		==Crystal structure of the CH domain of human CEP44==
-	<StructureSection load='7pt5' size='340' side='right'caption='[[7pt5]]' scene=''>	+	<StructureSection load='7pt5' size='340' side='right'caption='[[7pt5]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PT5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PT5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pt5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PT5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PT5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pt5 OCA], [https://pdbe.org/7pt5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pt5 RCSB], [https://www.ebi.ac.uk/pdbsum/7pt5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pt5 ProSAT]</span></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pt5 OCA], [https://pdbe.org/7pt5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pt5 RCSB], [https://www.ebi.ac.uk/pdbsum/7pt5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pt5 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/CEP44_HUMAN CEP44_HUMAN]] Centriole-enriched microtubule-binding protein involved in centriole biogenesis. In collaboration with CEP295 and POC1B, is required for the centriole-to-centrosome conversion by ensuring the formation of bona fide centriole wall (PubMed:32060285). Functions as a linker component that maintains centrosome cohesion. Associates with CROCC and regulates its stability and localization to the centrosome (PubMed:31974111).<ref>PMID:31974111</ref> <ref>PMID:32060285</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Obtaining the high-resolution structures of proteins and their complexes is a crucial aspect of understanding the mechanisms of life. Experimental structure determination methods are time-consuming, expensive and cannot keep pace with the growing number of protein sequences available through genomic DNA sequencing. Thus, the ability to accurately predict the structure of proteins from their sequence is a holy grail of structural and computational biology that would remove a bottleneck in our efforts to understand as well as rationally engineer living systems. Recent advances in protein structure prediction, in particular the breakthrough with the AI-based tool AlphaFold2 (AF2), hold promise for achieving this goal, but the practical utility of AF2 remains to be explored. Focusing on proteins with essential roles in centrosome and centriole biogenesis, we demonstrate the quality and usability of the AF2 prediction models and we show that they can provide important insights into the modular organization of two key players in this process, CEP192 and CEP44. Furthermore, we used the AF2 algorithm to elucidate and then experimentally validate previously unknown prime features in the structure of TTBK2 bound to CEP164, as well as the Chibby1-FAM92A complex for which no structural information was available to date. These findings have important implications in understanding the regulation and function of these complexes. Finally, we also discuss some practical limitations of AF2 and anticipate the implications for future research approaches in the centriole/centrosome field.
		+
		+	Structural validation and assessment of AlphaFold2 predictions for centrosomal and centriolar proteins and their complexes.,van Breugel M, Rosa E Silva I, Andreeva A Commun Biol. 2022 Apr 5;5(1):312. doi: 10.1038/s42003-022-03269-0. PMID:35383272<ref>PMID:35383272</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7pt5" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Rosa e~~ Silva I]]	+	[[Category: Breugel, M van]]
-	[[Category: ~~Van Breugel M~~]]	+	[[Category: Silva, I Rosa e]]
		+	[[Category: Cell cycle]]
		+	[[Category: Centriole]]
		+	[[Category: Centrosome]]
		+	[[Category: Microtubule-associated protein]]

OCA at 10:24, 6 April 2022

OCA — Wed, 06 Apr 2022 10:24:29 GMT

←Older revision		Revision as of 10:24, 6 April 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7pt5 ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of the CH domain of human CEP44==
-		+	<StructureSection load='7pt5' size='340' side='right'caption='[[7pt5]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PT5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PT5 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pt5 OCA], [https://pdbe.org/7pt5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pt5 RCSB], [https://www.ebi.ac.uk/pdbsum/7pt5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pt5 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Rosa e Silva I]]
		+	[[Category: Van Breugel M]]

OCA at 10:14, 13 October 2021

OCA — Wed, 13 Oct 2021 10:14:11 GMT

←Older revision		Revision as of 10:14, 13 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7pt5 is ON HOLD	+	The entry 7pt5 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7pt5" [edit=sysop:move=sysop]

OCA — Wed, 06 Oct 2021 04:06:45 GMT

Protected "7pt5" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:06, 6 October 2021

OCA: New page: '''Unreleased structure''' The entry 7pt5 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 06 Oct 2021 04:06:44 GMT

New page: '''Unreleased structure''' The entry 7pt5 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7pt5 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7pt5 - Revision history

OCA at 03:07, 21 April 2022

OCA at 10:24, 6 April 2022

OCA at 10:14, 13 October 2021

OCA: Protected "7pt5" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7pt5 is ON HOLD Authors: Description: Category: Unreleased Structures