OCA at 03:07, 21 April 2022

OCA — Thu, 21 Apr 2022 03:07:29 GMT

←Older revision		Revision as of 03:07, 21 April 2022
Line 1:		Line 1:

	==Crystal structure of the SPD-2 domain of human CEP192==		==Crystal structure of the SPD-2 domain of human CEP192==
-	<StructureSection load='7ptb' size='340' side='right'caption='[[7ptb]]' scene=''>	+	<StructureSection load='7ptb' size='340' side='right'caption='[[7ptb]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PTB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ptb]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PTB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ptb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ptb OCA], [https://pdbe.org/7ptb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ptb RCSB], [https://www.ebi.ac.uk/pdbsum/7ptb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ptb ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ptb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ptb OCA], [https://pdbe.org/7ptb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ptb RCSB], [https://www.ebi.ac.uk/pdbsum/7ptb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ptb ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/CE192_HUMAN CE192_HUMAN]] Required for mitotic centrosome maturation and bipolar spindle assembly (PubMed:25042804, PubMed:17980596, PubMed:18207742). Appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication (PubMed:25042804, PubMed:17980596, PubMed:18207742). Centrosome-specific activating scaffold for AURKA and PLK1 (PubMed:25042804).<ref>PMID:17980596</ref> <ref>PMID:18207742</ref> <ref>PMID:25042804</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Obtaining the high-resolution structures of proteins and their complexes is a crucial aspect of understanding the mechanisms of life. Experimental structure determination methods are time-consuming, expensive and cannot keep pace with the growing number of protein sequences available through genomic DNA sequencing. Thus, the ability to accurately predict the structure of proteins from their sequence is a holy grail of structural and computational biology that would remove a bottleneck in our efforts to understand as well as rationally engineer living systems. Recent advances in protein structure prediction, in particular the breakthrough with the AI-based tool AlphaFold2 (AF2), hold promise for achieving this goal, but the practical utility of AF2 remains to be explored. Focusing on proteins with essential roles in centrosome and centriole biogenesis, we demonstrate the quality and usability of the AF2 prediction models and we show that they can provide important insights into the modular organization of two key players in this process, CEP192 and CEP44. Furthermore, we used the AF2 algorithm to elucidate and then experimentally validate previously unknown prime features in the structure of TTBK2 bound to CEP164, as well as the Chibby1-FAM92A complex for which no structural information was available to date. These findings have important implications in understanding the regulation and function of these complexes. Finally, we also discuss some practical limitations of AF2 and anticipate the implications for future research approaches in the centriole/centrosome field.
		+
		+	Structural validation and assessment of AlphaFold2 predictions for centrosomal and centriolar proteins and their complexes.,van Breugel M, Rosa E Silva I, Andreeva A Commun Biol. 2022 Apr 5;5(1):312. doi: 10.1038/s42003-022-03269-0. PMID:35383272<ref>PMID:35383272</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ptb" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Rosa e~~ Silva I]]	+	[[Category: Breugel, M van]]
-	[[Category: ~~Van Breugel M~~]]	+	[[Category: Silva, I Rosa e]]
		+	[[Category: Cell cycle]]
		+	[[Category: Cell-cycle]]
		+	[[Category: Centriole]]
		+	[[Category: Centrosome]]
		+	[[Category: Pericentriolar material]]

OCA at 10:24, 6 April 2022

OCA — Wed, 06 Apr 2022 10:24:32 GMT

←Older revision		Revision as of 10:24, 6 April 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7ptb ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of the SPD-2 domain of human CEP192==
-		+	<StructureSection load='7ptb' size='340' side='right'caption='[[7ptb]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PTB FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ptb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ptb OCA], [https://pdbe.org/7ptb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ptb RCSB], [https://www.ebi.ac.uk/pdbsum/7ptb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ptb ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Rosa e Silva I]]
		+	[[Category: Van Breugel M]]

OCA at 10:14, 13 October 2021

OCA — Wed, 13 Oct 2021 10:14:13 GMT

←Older revision		Revision as of 10:14, 13 October 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ptb is ON HOLD	+	The entry 7ptb is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7ptb" [edit=sysop:move=sysop]

OCA — Wed, 06 Oct 2021 04:07:06 GMT

Protected "7ptb" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:07, 6 October 2021

OCA: New page: '''Unreleased structure''' The entry 7ptb is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 06 Oct 2021 04:07:05 GMT

New page: '''Unreleased structure''' The entry 7ptb is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ptb is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7ptb - Revision history

OCA at 03:07, 21 April 2022

OCA at 10:24, 6 April 2022

OCA at 10:14, 13 October 2021

OCA: Protected "7ptb" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7ptb is ON HOLD Authors: Description: Category: Unreleased Structures