7pze - Revision history

OCA at 07:52, 1 May 2024

OCA — Wed, 01 May 2024 07:52:42 GMT

←Older revision		Revision as of 07:52, 1 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7pze]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ustilago_maydis Ustilago maydis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PZE FirstGlance]. <br>		<table><tr><td colspan='2'>[[7pze]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ustilago_maydis Ustilago maydis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PZE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pze OCA], [https://pdbe.org/7pze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pze RCSB], [https://www.ebi.ac.uk/pdbsum/7pze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pze ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pze OCA], [https://pdbe.org/7pze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pze RCSB], [https://www.ebi.ac.uk/pdbsum/7pze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pze ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/RRM4_USTMA RRM4_USTMA] Key RNA-binding protein involved in the formation of polar-growing hyphae which is essential for infection by the plant pathogen (PubMed:15643068, PubMed:17105762, PubMed:19494833). During filamentation, assembles into particles that shuttle bidirectionally along microtubules to both poles (PubMed:17105762, PubMed:19494833, PubMed:30738139). The RRM4 transport particles are part of the endosomal mRNP transport that regulates polarity of the infectious hyphae by transporting distinct mRNAs encoding, for example, the ubiquitin fusion protein UBI1, the small G protein RHO3, or the septin CDC3, from the nucleus to cell poles (PubMed:17105762, PubMed:19494833, PubMed:22357951, PubMed:24355572, PubMed:25985087, PubMed:30738139). Recognizes a broad spectrum of cargo mRNAs and precisely binds at stop codons, which constitute landmark sites of translation, suggesting an intimate connection of mRNA transport and translation (PubMed:30552148). Binds also to the specific binding motif UAUG of cargo mRNAs via its third RRM (PubMed:30552148). Plus-end-directed KIN3, a kinesin-3 type motor, mediates anterograde transport of RRM4-containing mRNPs whereas split dynein DYM1-DYN2 functions in retrograde movement of mRNPs (PubMed:22357951).<ref>PMID:15643068</ref> <ref>PMID:17105762</ref> <ref>PMID:19494833</ref> <ref>PMID:22357951</ref> <ref>PMID:24355572</ref> <ref>PMID:25985087</ref> <ref>PMID:30552148</ref> <ref>PMID:30738139</ref>		[https://www.uniprot.org/uniprot/RRM4_USTMA RRM4_USTMA] Key RNA-binding protein involved in the formation of polar-growing hyphae which is essential for infection by the plant pathogen (PubMed:15643068, PubMed:17105762, PubMed:19494833). During filamentation, assembles into particles that shuttle bidirectionally along microtubules to both poles (PubMed:17105762, PubMed:19494833, PubMed:30738139). The RRM4 transport particles are part of the endosomal mRNP transport that regulates polarity of the infectious hyphae by transporting distinct mRNAs encoding, for example, the ubiquitin fusion protein UBI1, the small G protein RHO3, or the septin CDC3, from the nucleus to cell poles (PubMed:17105762, PubMed:19494833, PubMed:22357951, PubMed:24355572, PubMed:25985087, PubMed:30738139). Recognizes a broad spectrum of cargo mRNAs and precisely binds at stop codons, which constitute landmark sites of translation, suggesting an intimate connection of mRNA transport and translation (PubMed:30552148). Binds also to the specific binding motif UAUG of cargo mRNAs via its third RRM (PubMed:30552148). Plus-end-directed KIN3, a kinesin-3 type motor, mediates anterograde transport of RRM4-containing mRNPs whereas split dynein DYM1-DYN2 functions in retrograde movement of mRNPs (PubMed:22357951).<ref>PMID:15643068</ref> <ref>PMID:17105762</ref> <ref>PMID:19494833</ref> <ref>PMID:22357951</ref> <ref>PMID:24355572</ref> <ref>PMID:25985087</ref> <ref>PMID:30552148</ref> <ref>PMID:30738139</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Spatiotemporal expression can be achieved by transport and translation of mRNAs at defined subcellular sites. An emerging mechanism mediating mRNA trafficking is microtubule-dependent co-transport on shuttling endosomes. Although progress has been made in identifying various components of the endosomal mRNA transport machinery, a mechanistic understanding of how these RNA-binding proteins are connected to endosomes is still lacking. Here, we demonstrate that a flexible MademoiseLLE (MLLE) domain platform within RNA-binding protein Rrm4 of Ustilago maydis is crucial for endosomal attachment. Our structure/function analysis uncovered three MLLE domains at the C-terminus of Rrm4 with a functionally defined hierarchy. MLLE3 recognises two PAM2-like sequences of the adaptor protein Upa1 and is essential for endosomal shuttling of Rrm4. MLLE1 and MLLE2 are most likely accessory domains exhibiting a variable binding mode for interaction with currently unknown partners. Thus, endosomal attachment of the mRNA transporter is orchestrated by a sophisticated MLLE domain binding platform.
-
-	A MademoiseLLE domain binding platform links the key RNA transporter to endosomes.,Devan SK, Schott-Verdugo S, Muntjes K, Bismar L, Reiners J, Hachani E, Schmitt L, Hoppner A, Smits SH, Gohlke H, Feldbrugge M PLoS Genet. 2022 Jun 21;18(6):e1010269. doi: 10.1371/journal.pgen.1010269. , eCollection 2022 Jun. PMID:35727840<ref>PMID:35727840</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 7pze" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:14, 28 December 2022

OCA — Wed, 28 Dec 2022 09:14:00 GMT

←Older revision		Revision as of 09:14, 28 December 2022
Line 1:		Line 1:

	==MademoiseLLE domain 2 of Rrm4 from Ustilago maydis==		==MademoiseLLE domain 2 of Rrm4 from Ustilago maydis==
-	<StructureSection load='7pze' size='340' side='right'caption='[[7pze]]' scene=''>	+	<StructureSection load='7pze' size='340' side='right'caption='[[7pze]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PZE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pze]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ustilago_maydis Ustilago maydis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PZE FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pze OCA], [https://pdbe.org/7pze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pze RCSB], [https://www.ebi.ac.uk/pdbsum/7pze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pze ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pze OCA], [https://pdbe.org/7pze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pze RCSB], [https://www.ebi.ac.uk/pdbsum/7pze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pze ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RRM4_USTMA RRM4_USTMA] Key RNA-binding protein involved in the formation of polar-growing hyphae which is essential for infection by the plant pathogen (PubMed:15643068, PubMed:17105762, PubMed:19494833). During filamentation, assembles into particles that shuttle bidirectionally along microtubules to both poles (PubMed:17105762, PubMed:19494833, PubMed:30738139). The RRM4 transport particles are part of the endosomal mRNP transport that regulates polarity of the infectious hyphae by transporting distinct mRNAs encoding, for example, the ubiquitin fusion protein UBI1, the small G protein RHO3, or the septin CDC3, from the nucleus to cell poles (PubMed:17105762, PubMed:19494833, PubMed:22357951, PubMed:24355572, PubMed:25985087, PubMed:30738139). Recognizes a broad spectrum of cargo mRNAs and precisely binds at stop codons, which constitute landmark sites of translation, suggesting an intimate connection of mRNA transport and translation (PubMed:30552148). Binds also to the specific binding motif UAUG of cargo mRNAs via its third RRM (PubMed:30552148). Plus-end-directed KIN3, a kinesin-3 type motor, mediates anterograde transport of RRM4-containing mRNPs whereas split dynein DYM1-DYN2 functions in retrograde movement of mRNPs (PubMed:22357951).<ref>PMID:15643068</ref> <ref>PMID:17105762</ref> <ref>PMID:19494833</ref> <ref>PMID:22357951</ref> <ref>PMID:24355572</ref> <ref>PMID:25985087</ref> <ref>PMID:30552148</ref> <ref>PMID:30738139</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Spatiotemporal expression can be achieved by transport and translation of mRNAs at defined subcellular sites. An emerging mechanism mediating mRNA trafficking is microtubule-dependent co-transport on shuttling endosomes. Although progress has been made in identifying various components of the endosomal mRNA transport machinery, a mechanistic understanding of how these RNA-binding proteins are connected to endosomes is still lacking. Here, we demonstrate that a flexible MademoiseLLE (MLLE) domain platform within RNA-binding protein Rrm4 of Ustilago maydis is crucial for endosomal attachment. Our structure/function analysis uncovered three MLLE domains at the C-terminus of Rrm4 with a functionally defined hierarchy. MLLE3 recognises two PAM2-like sequences of the adaptor protein Upa1 and is essential for endosomal shuttling of Rrm4. MLLE1 and MLLE2 are most likely accessory domains exhibiting a variable binding mode for interaction with currently unknown partners. Thus, endosomal attachment of the mRNA transporter is orchestrated by a sophisticated MLLE domain binding platform.
		+
		+	A MademoiseLLE domain binding platform links the key RNA transporter to endosomes.,Devan SK, Schott-Verdugo S, Muntjes K, Bismar L, Reiners J, Hachani E, Schmitt L, Hoppner A, Smits SH, Gohlke H, Feldbrugge M PLoS Genet. 2022 Jun 21;18(6):e1010269. doi: 10.1371/journal.pgen.1010269. , eCollection 2022 Jun. PMID:35727840<ref>PMID:35727840</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7pze" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Ustilago maydis]]
	[[Category: Devans S]]		[[Category: Devans S]]
	[[Category: Feldbruegge M]]		[[Category: Feldbruegge M]]

OCA at 05:10, 15 June 2022

OCA — Wed, 15 Jun 2022 05:10:38 GMT

←Older revision		Revision as of 05:10, 15 June 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7pze ~~is ON HOLD until Oct 12 2023~~	+	==MademoiseLLE domain 2 of Rrm4 from Ustilago maydis==
-		+	<StructureSection load='7pze' size='340' side='right'caption='[[7pze]]' scene=''>
-	~~Authors~~: ~~Devans, S~~.~~, Schott~~-~~Verdugo, s~~.~~, Muentjes, K~~.~~, Olgeiser, L~~.~~, Reiners, J~~., ~~Schmitt, L~~., ~~Hoeppner, A~~., ~~Smits, S~~.H., ~~Gohlke, H~~., ~~Feldbruegge, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PZE FirstGlance]. <br>
-	~~Description: MademoiseLLE domain 2 of Rrm4 from Ustilago maydis~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pze OCA], [https://pdbe.org/7pze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pze RCSB], [https://www.ebi.ac.uk/pdbsum/7pze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pze ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Feldbruegge, M~~]]	+	__TOC__
-	[[Category: ~~Hoeppner, A~~]]	+	</StructureSection>
-	[[Category: ~~Olgeiser, L~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Devans, S~~]]	+	[[Category: Devans S]]
-	[[Category: ~~Smits, S.H~~]]	+	[[Category: Feldbruegge M]]
-	[[Category: ~~Gohlke, H~~]]	+	[[Category: Gohlke H]]
-	[[Category: Reiners, J]]	+	[[Category: Hoeppner A]]
-	[[Category: ~~Muentjes, K~~]]	+	[[Category: Muentjes K]]
-	[[Category: Schott-Verdugo~~, S~~]]	+	[[Category: Olgeiser L]]
-	[[Category: ~~Schmitt, L~~]]	+	[[Category: Reiners J]]
		+	[[Category: Schmitt L]]
		+	[[Category: Schott-Verdugo s]]
		+	[[Category: Smits SH]]

OCA at 12:01, 17 November 2021

OCA — Wed, 17 Nov 2021 12:01:05 GMT

←Older revision		Revision as of 12:01, 17 November 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7pze is ON HOLD	+	The entry 7pze is ON HOLD until Oct 12 2023

	Authors: Devans, S., Schott-Verdugo, s., Muentjes, K., Olgeiser, L., Reiners, J., Schmitt, L., Hoeppner, A., Smits, S.H., Gohlke, H., Feldbruegge, M.		Authors: Devans, S., Schott-Verdugo, s., Muentjes, K., Olgeiser, L., Reiners, J., Schmitt, L., Hoeppner, A., Smits, S.H., Gohlke, H., Feldbruegge, M.
Line 7:		Line 7:
	Description: MademoiseLLE domain 2 of Rrm4 from Ustilago maydis		Description: MademoiseLLE domain 2 of Rrm4 from Ustilago maydis
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Gohlke~~, H]]	+	[[Category: Feldbruegge, M]]
	[[Category: Hoeppner, A]]		[[Category: Hoeppner, A]]
	[[Category: Olgeiser, L]]		[[Category: Olgeiser, L]]
	[[Category: Devans, S]]		[[Category: Devans, S]]
		+	[[Category: Smits, S.H]]
		+	[[Category: Gohlke, H]]
	[[Category: Reiners, J]]		[[Category: Reiners, J]]
	[[Category: Muentjes, K]]		[[Category: Muentjes, K]]
-	[[Category: Schmitt, L]]
-	[[Category: Feldbruegge, M]]
-	[[Category: Smits, S.H]]
	[[Category: Schott-Verdugo, S]]		[[Category: Schott-Verdugo, S]]
		+	[[Category: Schmitt, L]]

OCA: Protected "7pze" [edit=sysop:move=sysop]

OCA — Wed, 20 Oct 2021 14:59:10 GMT

Protected "7pze" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:59, 20 October 2021

OCA: New page: '''Unreleased structure''' The entry 7pze is ON HOLD Authors: Devans, S., Schott-Verdugo, s., Muentjes, K., Olgeiser, L., Reiners, J., Schmitt, L., Hoeppner, A., Smits, S.H., Gohlke, H....

OCA — Wed, 20 Oct 2021 14:59:08 GMT

New page: '''Unreleased structure''' The entry 7pze is ON HOLD Authors: Devans, S., Schott-Verdugo, s., Muentjes, K., Olgeiser, L., Reiners, J., Schmitt, L., Hoeppner, A., Smits, S.H., Gohlke, H....

New page

'''Unreleased structure'''

The entry 7pze is ON HOLD

Authors: Devans, S., Schott-Verdugo, s., Muentjes, K., Olgeiser, L., Reiners, J., Schmitt, L., Hoeppner, A., Smits, S.H., Gohlke, H., Feldbruegge, M.

Description: MademoiseLLE domain 2 of Rrm4 from Ustilago maydis
[[Category: Unreleased Structures]]
[[Category: Gohlke, H]]
[[Category: Hoeppner, A]]
[[Category: Olgeiser, L]]
[[Category: Devans, S]]
[[Category: Reiners, J]]
[[Category: Muentjes, K]]
[[Category: Schmitt, L]]
[[Category: Feldbruegge, M]]
[[Category: Smits, S.H]]
[[Category: Schott-Verdugo, S]]