7q07 - Revision history

OCA at 13:11, 1 February 2024

OCA — Thu, 01 Feb 2024 13:11:47 GMT

←Older revision		Revision as of 13:11, 1 February 2024
Line 1:		Line 1:

	==Ketol-acid reductoisomerase from Methanothermococcus thermolithotrophicus in the open state with NADP and tartrate==		==Ketol-acid reductoisomerase from Methanothermococcus thermolithotrophicus in the open state with NADP and tartrate==
-	<StructureSection load='7q07' size='340' side='right'caption='[[7q07]]' scene=''>	+	<StructureSection load='7q07' size='340' side='right'caption='[[7q07]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q07 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7q07]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus_DSM_2095 Methanothermococcus thermolithotrophicus DSM 2095]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q07 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q07 OCA], [https://pdbe.org/7q07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q07 RCSB], [https://www.ebi.ac.uk/pdbsum/7q07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q07 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q07 OCA], [https://pdbe.org/7q07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q07 RCSB], [https://www.ebi.ac.uk/pdbsum/7q07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q07 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ketol-acid reductoisomerase (KARI) orchestrates the biosynthesis of branched-chain amino acids, an elementary reaction in prototrophic organisms as well as a valuable process in biotechnology. Bacterial KARIs belonging to class I organise as dimers or dodecamers and were intensively studied to understand their remarkable specificity towards NADH or NADPH, but also to develop antibiotics. Here, we present the first structural study on a KARI natively isolated from a methanogenic archaea. The dodecameric structure of 0.44-MDa was obtained in two different conformations, an open and close state refined to a resolution of 2.2-A and 2.1-A, respectively. These structures illustrate the conformational movement required for substrate and coenzyme binding. While the close state presents the complete NADP bound in front of a partially occupied Mg(2+)-site, the Mg(2+)-free open state contains a tartrate at the nicotinamide location and a bound NADP with the adenine-nicotinamide protruding out of the active site. Structural comparisons show a very high conservation of the active site environment and detailed analyses point towards few specific residues required for the dodecamerisation. These residues are not conserved in other dodecameric KARIs that stabilise their trimeric interface differently, suggesting that dodecamerisation, the cellular role of which is still unknown, might have occurred several times in the evolution of KARIs.
		+
		+	Structural Rearrangements of a Dodecameric Ketol-Acid Reductoisomerase Isolated from a Marine Thermophilic Methanogen.,Lemaire ON, Muller MC, Kahnt J, Wagner T Biomolecules. 2021 Nov 11;11(11). pii: biom11111679. doi: 10.3390/biom11111679. PMID:34827677<ref>PMID:34827677</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7q07" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ketol-acid reductoisomerase 3D structures\|Ketol-acid reductoisomerase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Methanothermococcus thermolithotrophicus DSM 2095]]
	[[Category: Lemaire ON]]		[[Category: Lemaire ON]]
	[[Category: Mueller M]]		[[Category: Mueller M]]
	[[Category: Wagner T]]		[[Category: Wagner T]]

OCA at 09:58, 8 December 2021

OCA — Wed, 08 Dec 2021 09:58:06 GMT

←Older revision		Revision as of 09:58, 8 December 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7q07 is ON HOLD until Paper Publication~~	+	==Ketol-acid reductoisomerase from Methanothermococcus thermolithotrophicus in the open state with NADP and tartrate==
-		+	<StructureSection load='7q07' size='340' side='right'caption='[[7q07]]' scene=''>
-	~~Authors: Lemaire, O.N., Mueller, M., Wagner, T.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q07 FirstGlance]. <br>
-	~~Description:~~ Ketol-acid reductoisomerase from Methanothermococcus thermolithotrophicus in the open state with NADP and tartrate	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q07 OCA], [https://pdbe.org/7q07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q07 RCSB], [https://www.ebi.ac.uk/pdbsum/7q07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q07 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Wagner, T~~]]	+	__TOC__
-	[[Category: Mueller, M]]	+	</StructureSection>
-	[[Category: ~~Lemaire, O.N~~]]	+	[[Category: Large Structures]]
		+	[[Category: Lemaire ON]]
		+	[[Category: Mueller M]]
		+	[[Category: Wagner T]]

OCA at 12:42, 3 November 2021

OCA — Wed, 03 Nov 2021 12:42:44 GMT

←Older revision		Revision as of 12:42, 3 November 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7q07 is ON HOLD	+	The entry 7q07 is ON HOLD until Paper Publication

	Authors: Lemaire, O.N., Mueller, M., Wagner, T.		Authors: Lemaire, O.N., Mueller, M., Wagner, T.

OCA: Protected "7q07" [edit=sysop:move=sysop]

OCA — Wed, 20 Oct 2021 15:01:44 GMT

Protected "7q07" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:01, 20 October 2021

OCA: New page: '''Unreleased structure''' The entry 7q07 is ON HOLD Authors: Lemaire, O.N., Mueller, M., Wagner, T. Description: Ketol-acid reductoisomerase from Methanothermococcus thermolithotrophi...

OCA — Wed, 20 Oct 2021 15:01:43 GMT

New page: '''Unreleased structure''' The entry 7q07 is ON HOLD Authors: Lemaire, O.N., Mueller, M., Wagner, T. Description: Ketol-acid reductoisomerase from Methanothermococcus thermolithotrophi...

New page

'''Unreleased structure'''

The entry 7q07 is ON HOLD

Authors: Lemaire, O.N., Mueller, M., Wagner, T.

Description: Ketol-acid reductoisomerase from Methanothermococcus thermolithotrophicus in the open state with NADP and tartrate
[[Category: Unreleased Structures]]
[[Category: Wagner, T]]
[[Category: Mueller, M]]
[[Category: Lemaire, O.N]]