7q3t - Revision history

OCA at 13:13, 1 February 2024

OCA — Thu, 01 Feb 2024 13:13:31 GMT

←Older revision		Revision as of 13:13, 1 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7q3t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q3T FirstGlance]. <br>		<table><tr><td colspan='2'>[[7q3t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q3T FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.788Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q3t OCA], [https://pdbe.org/7q3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q3t RCSB], [https://www.ebi.ac.uk/pdbsum/7q3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q3t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q3t OCA], [https://pdbe.org/7q3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q3t RCSB], [https://www.ebi.ac.uk/pdbsum/7q3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q3t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/D6QLY0_KLEPN D6QLY0_KLEPN]]	+	[https://www.uniprot.org/uniprot/D6QLY0_KLEPN D6QLY0_KLEPN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 03:50, 8 September 2022

OCA — Thu, 08 Sep 2022 03:50:22 GMT

←Older revision		Revision as of 03:50, 8 September 2022
Line 3:		Line 3:
	<StructureSection load='7q3t' size='340' side='right'caption='[[7q3t]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='7q3t' size='340' side='right'caption='[[7q3t]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7q3t]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q3T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7q3t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q3T FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q3t OCA], [https://pdbe.org/7q3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q3t RCSB], [https://www.ebi.ac.uk/pdbsum/7q3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q3t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q3t OCA], [https://pdbe.org/7q3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q3t RCSB], [https://www.ebi.ac.uk/pdbsum/7q3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q3t ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/D6QLY0_KLEPN D6QLY0_KLEPN]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Klebsiella pneumoniae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Beis, K]]	+	[[Category: Beis K]]
-	[[Category: Kwong, H]]	+	[[Category: Kwong H]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Outer membrane porin~~]]	+

OCA at 05:00, 3 August 2022

OCA — Wed, 03 Aug 2022 05:00:02 GMT

←Older revision		Revision as of 05:00, 3 August 2022
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	==Crystal structure of the OmpK36 D insertion chimera from Klebsiella pneumonia==		==Crystal structure of the OmpK36 D insertion chimera from Klebsiella pneumonia==
-	<StructureSection load='7q3t' size='340' side='right'caption='[[7q3t]]' scene=''>	+	<StructureSection load='7q3t' size='340' side='right'caption='[[7q3t]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q3T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7q3t]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q3T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q3t OCA], [https://pdbe.org/7q3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q3t RCSB], [https://www.ebi.ac.uk/pdbsum/7q3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q3t ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q3t OCA], [https://pdbe.org/7q3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q3t RCSB], [https://www.ebi.ac.uk/pdbsum/7q3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q3t ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mutations in outer membrane porins act in synergy with carbapenemase enzymes to increase carbapenem resistance in the important nosocomial pathogen, Klebsiella pneumoniae (KP). A key example is a di-amino acid insertion, Glycine-Aspartate (GD), in the extracellular loop 3 (L3) region of OmpK36 which constricts the pore and restricts entry of carbapenems into the bacterial cell. Here we combined genomic and experimental approaches to characterise the diversity, spread and impact of different L3 insertion types in OmpK36. We identified L3 insertions in 3588 (24.1%) of 14,888 KP genomes with an intact ompK36 gene from a global collection. GD insertions were most common, with a high concentration in the ST258/512 clone that has spread widely in Europe and the Americas. Aspartate (D) and Threonine-Aspartate (TD) insertions were prevalent in genomes from Asia, due in part to acquisitions by KP sequence types ST16 and ST231 and subsequent clonal expansions. By solving the crystal structures of novel OmpK36 variants, we found that the TD insertion causes a pore constriction of 41%, significantly greater than that achieved by GD (10%) or D (8%), resulting in the highest levels of resistance to selected antibiotics. We show that in the absence of antibiotics KP mutants harbouring these L3 insertions exhibit both an in vitro and in vivo competitive disadvantage relative to the isogenic parental strain expressing wild type OmpK36. We propose that this explains the reversion of GD and TD insertions observed at low frequency among KP genomes. Finally, we demonstrate that strains expressing L3 insertions remain susceptible to drugs targeting carbapenemase-producing KP, including novel beta lactam-beta lactamase inhibitor combinations. This study provides a contemporary global view of OmpK36-mediated resistance mechanisms in KP, integrating surveillance and experimental data to guide treatment and drug development strategies.
		+
		+	Widespread emergence of OmpK36 loop 3 insertions among multidrug-resistant clones of Klebsiella pneumoniae.,David S, Wong JLC, Sanchez-Garrido J, Kwong HS, Low WW, Morecchiato F, Giani T, Rossolini GM, Brett SJ, Clements A, Beis K, Aanensen DM, Frankel G PLoS Pathog. 2022 Jul 11;18(7):e1010334. doi: 10.1371/journal.ppat.1010334., eCollection 2022 Jul. PMID:35816554<ref>PMID:35816554</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7q3t" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Beis K]]	+	[[Category: Beis, K]]
-	[[Category: Kwong H]]	+	[[Category: Kwong, H]]
		+	[[Category: Membrane protein]]
		+	[[Category: Outer membrane porin]]

OCA at 16:12, 6 July 2022

OCA — Wed, 06 Jul 2022 16:12:43 GMT

←Older revision		Revision as of 16:12, 6 July 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7q3t is ON HOLD until Paper Publication~~	+	==Crystal structure of the OmpK36 D insertion chimera from Klebsiella pneumonia==
-		+	<StructureSection load='7q3t' size='340' side='right'caption='[[7q3t]]' scene=''>
-	~~Authors: Kwong, H., Beis, K.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q3T FirstGlance]. <br>
-	~~Description:~~ Crystal structure of the OmpK36 D insertion chimera from Klebsiella pneumonia	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q3t OCA], [https://pdbe.org/7q3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q3t RCSB], [https://www.ebi.ac.uk/pdbsum/7q3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q3t ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Kwong~~, H]]	+	__TOC__
-	[[Category: Beis, K]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Beis K]]
		+	[[Category: Kwong H]]

OCA at 13:51, 22 December 2021

OCA — Wed, 22 Dec 2021 13:51:06 GMT

←Older revision		Revision as of 13:51, 22 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7q3t is ON HOLD	+	The entry 7q3t is ON HOLD until Paper Publication

	Authors: Kwong, H., Beis, K.		Authors: Kwong, H., Beis, K.

OCA at 05:02, 1 December 2021

OCA — Wed, 01 Dec 2021 05:02:21 GMT

←Older revision		Revision as of 05:02, 1 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7q3t is ON HOLD ~~until sometime in the future~~	+	The entry 7q3t is ON HOLD

	Authors: Kwong, H., Beis, K.		Authors: Kwong, H., Beis, K.

OCA at 12:02, 17 November 2021

OCA — Wed, 17 Nov 2021 12:02:23 GMT

←Older revision		Revision as of 12:02, 17 November 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7q3t is ON HOLD	+	The entry 7q3t is ON HOLD until sometime in the future

	Authors: Kwong, H., Beis, K.		Authors: Kwong, H., Beis, K.

OCA: Protected "7q3t" [edit=sysop:move=sysop]

OCA — Wed, 03 Nov 2021 12:47:42 GMT

Protected "7q3t" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:47, 3 November 2021

OCA: New page: '''Unreleased structure''' The entry 7q3t is ON HOLD Authors: Kwong, H., Beis, K. Description: Crystal structure of the OmpK36 D insertion chimera from Klebsiella pneumonia [[Category:...

OCA — Wed, 03 Nov 2021 12:47:40 GMT

New page: '''Unreleased structure''' The entry 7q3t is ON HOLD Authors: Kwong, H., Beis, K. Description: Crystal structure of the OmpK36 D insertion chimera from Klebsiella pneumonia [[Category:...

New page

'''Unreleased structure'''

The entry 7q3t is ON HOLD

Authors: Kwong, H., Beis, K.

Description: Crystal structure of the OmpK36 D insertion chimera from Klebsiella pneumonia
[[Category: Unreleased Structures]]
[[Category: Kwong, H]]
[[Category: Beis, K]]