OCA at 13:15, 1 February 2024

OCA — Thu, 01 Feb 2024 13:15:06 GMT

←Older revision		Revision as of 13:15, 1 February 2024
Line 1:		Line 1:

-	====	+	==LIPIDIC CUBIC PHASE SERIAL FEMTOSECOND CRYSTALLOGRAPHY STRUCTURE OF A PHOTOSYNTHETIC REACTION CENTRE==
-	<StructureSection load='7q7p' size='340' side='right'caption='[[7q7p]]' scene=''>	+	<StructureSection load='7q7p' size='340' side='right'caption='[[7q7p]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7q7p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Blastochloris_viridis Blastochloris viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q7P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q7p OCA], [https://pdbe.org/7q7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q7p RCSB], [https://www.ebi.ac.uk/pdbsum/7q7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q7p ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCB:BACTERIOCHLOROPHYLL+B'>BCB</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MQ7:MENAQUINONE-7'>MQ7</scene>, <scene name='pdbligand=NS5:15-CIS-1,2-DIHYDRONEUROSPORENE'>NS5</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UQ1:UBIQUINONE-1'>UQ1</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q7p OCA], [https://pdbe.org/7q7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q7p RCSB], [https://www.ebi.ac.uk/pdbsum/7q7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q7p ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CYCR_BLAVI CYCR_BLAVI] The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor.<ref>PMID:10736158</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Serial crystallography is a rapidly growing method that can yield structural insights from microcrystals that were previously considered to be too small to be useful in conventional X-ray crystallography. Here, conditions for growing microcrystals of the photosynthetic reaction centre of Blastochloris viridis within a lipidic cubic phase (LCP) crystallization matrix that employ a seeding protocol utilizing detergent-grown crystals with a different crystal packing are described. LCP microcrystals diffracted to 2.25 A resolution when exposed to XFEL radiation, which is an improvement of 0.15 A over previous microcrystal forms. Ubiquinone was incorporated into the LCP crystallization media and the resulting electron density within the mobile Q(B) pocket is comparable to that of other cofactors within the structure. As such, LCP microcrystallization conditions will facilitate time-resolved diffraction studies of electron-transfer reactions to the mobile quinone, potentially allowing the observation of structural changes associated with the two electron-transfer reactions leading to complete reduction of the ubiquinone ligand.
		+
		+	Lipidic cubic phase serial femtosecond crystallography structure of a photosynthetic reaction centre.,Bath P, Banacore A, Borjesson P, Bosman R, Wickstrand C, Safari C, Dods R, Ghosh S, Dahl P, Ortolani G, Bjorg Ulfarsdottir T, Hammarin G, Garcia Bonete MJ, Vallejos A, Ostojic L, Edlund P, Linse JB, Andersson R, Nango E, Owada S, Tanaka R, Tono K, Joti Y, Nureki O, Luo F, James D, Nass K, Johnson PJM, Knopp G, Ozerov D, Cirelli C, Milne C, Iwata S, Branden G, Neutze R Acta Crystallogr D Struct Biol. 2022 Jun 1;78(Pt 6):698-708. doi: , 10.1107/S2059798322004144. Epub 2022 May 25. PMID:35647917<ref>PMID:35647917</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7q7p" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Blastochloris viridis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Baath P]]
		+	[[Category: Banacore A]]
		+	[[Category: Neutze R]]

OCA at 09:45, 22 June 2022

OCA — Wed, 22 Jun 2022 09:45:19 GMT

←Older revision		Revision as of 09:45, 22 June 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7q7p ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7q7p' size='340' side='right'caption='[[7q7p]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q7p OCA], [https://pdbe.org/7q7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q7p RCSB], [https://www.ebi.ac.uk/pdbsum/7q7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q7p ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7q7p" [edit=sysop:move=sysop]

OCA — Wed, 17 Nov 2021 12:06:05 GMT

Protected "7q7p" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:06, 17 November 2021

OCA: New page: '''Unreleased structure''' The entry 7q7p is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 17 Nov 2021 12:06:04 GMT

New page: '''Unreleased structure''' The entry 7q7p is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7q7p is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7q7p - Revision history

OCA at 13:15, 1 February 2024

OCA at 09:45, 22 June 2022

OCA: Protected "7q7p" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7q7p is ON HOLD Authors: Description: Category: Unreleased Structures