7q7x - Revision history

OCA at 13:15, 1 February 2024

OCA — Thu, 01 Feb 2024 13:15:15 GMT

←Older revision		Revision as of 13:15, 1 February 2024
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	==Crystal structure of the methyltransferase-ribozyme 1 (with 1-methyl-adenosine)==		==Crystal structure of the methyltransferase-ribozyme 1 (with 1-methyl-adenosine)==
-	<StructureSection load='7q7x' size='340' side='right'caption='[[7q7x]]' scene=''>	+	<StructureSection load='7q7x' size='340' side='right'caption='[[7q7x]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q7X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7q7x]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q7X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q7x OCA], [https://pdbe.org/7q7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q7x RCSB], [https://www.ebi.ac.uk/pdbsum/7q7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q7x ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MA:6-HYDRO-1-METHYLADENOSINE-5-MONOPHOSPHATE'>1MA</scene>, <scene name='pdbligand=GUN:GUANINE'>GUN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q7x OCA], [https://pdbe.org/7q7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q7x RCSB], [https://www.ebi.ac.uk/pdbsum/7q7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q7x ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RNA-catalyzed RNA methylation was recently shown to be part of the catalytic repertoire of ribozymes. The methyltransferase ribozyme MTR1 catalyzes the site-specific synthesis of 1-methyladenosine (m(1)A) in RNA, using O(6)-methylguanine (m(6)G) as a methyl group donor. Here, we report the crystal structure of MTR1 at a resolution of 2.8 A, which reveals a guanine-binding site reminiscent of natural guanine riboswitches. The structure represents the postcatalytic state of a split ribozyme in complex with the m(1)A-containing RNA product and the demethylated cofactor guanine. The structural data suggest the mechanistic involvement of a protonated cytidine in the methyl transfer reaction. A synergistic effect of two 2'-O-methylated ribose residues in the active site results in accelerated methyl group transfer. Supported by these results, it seems plausible that modified nucleotides may have enhanced early RNA catalysis and that metabolite-binding riboswitches may resemble inactivated ribozymes that have lost their catalytic activity during evolution.
		+
		+	Structure and mechanism of the methyltransferase ribozyme MTR1.,Scheitl CPM, Mieczkowski M, Schindelin H, Hobartner C Nat Chem Biol. 2022 May;18(5):547-555. doi: 10.1038/s41589-022-00976-x. Epub 2022, Mar 17. PMID:35301481<ref>PMID:35301481</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7q7x" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ribozyme 3D structures\|Ribozyme 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Synthetic construct]]
	[[Category: Hoebartner C]]		[[Category: Hoebartner C]]
	[[Category: Mieczkowski M]]		[[Category: Mieczkowski M]]

OCA at 14:48, 31 August 2022

OCA — Wed, 31 Aug 2022 14:48:27 GMT

←Older revision		Revision as of 14:48, 31 August 2022
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-	====	+	==Crystal structure of the methyltransferase-ribozyme 1 (with 1-methyl-adenosine)==
	<StructureSection load='7q7x' size='340' side='right'caption='[[7q7x]]' scene=''>		<StructureSection load='7q7x' size='340' side='right'caption='[[7q7x]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q7X FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q7x OCA], [https://pdbe.org/7q7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q7x RCSB], [https://www.ebi.ac.uk/pdbsum/7q7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q7x ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q7x OCA], [https://pdbe.org/7q7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q7x RCSB], [https://www.ebi.ac.uk/pdbsum/7q7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q7x ProSAT]</span></td></tr>
	</table>		</table>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Hoebartner C]]
		+	[[Category: Mieczkowski M]]

OCA at 11:18, 23 March 2022

OCA — Wed, 23 Mar 2022 11:18:40 GMT

←Older revision		Revision as of 11:18, 23 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7q7x ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7q7x' size='340' side='right'caption='[[7q7x]]' scene=''>
-	~~Authors~~: ~~Mieczkowski, M~~.~~, Hoebartner, C~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of the methyltransferase~~-~~ribozyme 1 (with 1-methyl-adenosine)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q7x OCA], [https://pdbe.org/7q7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q7x RCSB], [https://www.ebi.ac.uk/pdbsum/7q7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q7x ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Mieczkowski, M~~]]	+	__TOC__
-	[[Category: ~~Hoebartner, C~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 13:52, 22 December 2021

OCA — Wed, 22 Dec 2021 13:52:24 GMT

←Older revision		Revision as of 13:52, 22 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7q7x is ON HOLD	+	The entry 7q7x is ON HOLD until Paper Publication

	Authors: Mieczkowski, M., Hoebartner, C.		Authors: Mieczkowski, M., Hoebartner, C.

OCA: Protected "7q7x" [edit=sysop:move=sysop]

OCA — Wed, 17 Nov 2021 12:06:26 GMT

Protected "7q7x" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:06, 17 November 2021

OCA: New page: '''Unreleased structure''' The entry 7q7x is ON HOLD Authors: Mieczkowski, M., Hoebartner, C. Description: Crystal structure of the methyltransferase-ribozyme 1 (with 1-methyl-adenosin...

OCA — Wed, 17 Nov 2021 12:06:25 GMT

New page: '''Unreleased structure''' The entry 7q7x is ON HOLD Authors: Mieczkowski, M., Hoebartner, C. Description: Crystal structure of the methyltransferase-ribozyme 1 (with 1-methyl-adenosin...

New page

'''Unreleased structure'''

The entry 7q7x is ON HOLD

Authors: Mieczkowski, M., Hoebartner, C.

Description: Crystal structure of the methyltransferase-ribozyme 1 (with 1-methyl-adenosine)
[[Category: Unreleased Structures]]
[[Category: Mieczkowski, M]]
[[Category: Hoebartner, C]]