7qc9 - Revision history

OCA at 13:16, 1 February 2024

OCA — Thu, 01 Feb 2024 13:16:41 GMT

←Older revision		Revision as of 13:16, 1 February 2024
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	==HisF-C9A-D11E-V33A_L50H_I52H mutant in complex with Ni(II) from T. maritima==		==HisF-C9A-D11E-V33A_L50H_I52H mutant in complex with Ni(II) from T. maritima==
-	<StructureSection load='7qc9' size='340' side='right'caption='[[7qc9]]' scene=''>	+	<StructureSection load='7qc9' size='340' side='right'caption='[[7qc9]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QC9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7qc9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QC9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qc9 OCA], [https://pdbe.org/7qc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qc9 RCSB], [https://www.ebi.ac.uk/pdbsum/7qc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qc9 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qc9 OCA], [https://pdbe.org/7qc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qc9 RCSB], [https://www.ebi.ac.uk/pdbsum/7qc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qc9 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/HIS6_THEMA HIS6_THEMA] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.[HAMAP-Rule:MF_01013]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Engineering non-native metal active sites into proteins using canonical amino acids offers many advantages but is hampered by significant challenges. The TIM barrel protein, imidazole glycerol phosphate synthase from the hyperthermophilic organism Thermotoga maritima (tHisF), is well-suited for the construction of artificial metalloenzymes by this approach. To this end, we have generated a tHisF variant (tHisF(EHH)) with a Glu/His/His motif for metal ion coordination. Crystal structures of Zn(II):tHisF(EHH) and Ni(II):tHisF(EHH) reveal that both metal ions bind to the engineered histidines. However, the two metals bind at distinct sites with different geometries, demonstrating the adaptability of tHisF. Only Zn(II) additionally ligates the Glu residue and adopts a tetrahedral geometry. The pseudo-octahedral Ni(II) site comprises the two His and a native Ser residue. Ni(II):tHisF(EHH) catalyzes the oxidative cleavage of the flavanols quercetin and myricetin, providing an unprecedented example of an artificial metalloprotein with quercetinase activity.
		+
		+	An artificial metalloprotein with metal-adaptive coordination sites and Ni-dependent quercetinase activity.,Beaumet M, Dose A, Brauer A, Mahy JP, Ghattas W, Groll M, Hess CR J Inorg Biochem. 2022 Jul 6;235:111914. doi: 10.1016/j.jinorgbio.2022.111914. PMID:35841720<ref>PMID:35841720</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7qc9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Thermotoga maritima MSB8]]
	[[Category: Beaumet M]]		[[Category: Beaumet M]]
	[[Category: Braeuer A]]		[[Category: Braeuer A]]

OCA at 04:59, 10 August 2022

OCA — Wed, 10 Aug 2022 04:59:36 GMT

←Older revision		Revision as of 04:59, 10 August 2022
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-	'''Unreleased structure'''

-	~~The entry 7qc9 is ON HOLD until Paper Publication~~	+	==HisF-C9A-D11E-V33A_L50H_I52H mutant in complex with Ni(II) from T. maritima==
-		+	<StructureSection load='7qc9' size='340' side='right'caption='[[7qc9]]' scene=''>
-	~~Authors: Beaumet, M., Dose, A., Braeuer, A., Mahy, J., Ghattas, W., Groll, M., Hess, C.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QC9 FirstGlance]. <br>
-	~~Description:~~ HisF-C9A-D11E-V33A_L50H_I52H mutant in complex with Ni(II) from T. maritima	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qc9 OCA], [https://pdbe.org/7qc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qc9 RCSB], [https://www.ebi.ac.uk/pdbsum/7qc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qc9 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Hess~~, C]]	+	__TOC__
-	[[Category: ~~Dose, A~~]]	+	</StructureSection>
-	[[Category: Beaumet, M]]	+	[[Category: Large Structures]]
-	[[Category: ~~Groll, M~~]]	+	[[Category: Beaumet M]]
-	[[Category: Ghattas, W]]	+	[[Category: Braeuer A]]
-	[[Category: ~~Braeuer, A~~]]	+	[[Category: Dose A]]
-	[[Category: Mahy, J]]	+	[[Category: Ghattas W]]
		+	[[Category: Groll M]]
		+	[[Category: Hess C]]
		+	[[Category: Mahy J]]

OCA at 06:29, 22 June 2022

OCA — Wed, 22 Jun 2022 06:29:52 GMT

←Older revision		Revision as of 06:29, 22 June 2022
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Hess, C]]		[[Category: Hess, C]]
		+	[[Category: Dose, A]]
	[[Category: Beaumet, M]]		[[Category: Beaumet, M]]
	[[Category: Groll, M]]		[[Category: Groll, M]]
Line 13:		Line 14:
	[[Category: Braeuer, A]]		[[Category: Braeuer, A]]
	[[Category: Mahy, J]]		[[Category: Mahy, J]]
-	[[Category: Dose, A]]

OCA at 05:16, 27 January 2022

OCA — Thu, 27 Jan 2022 05:16:15 GMT

←Older revision		Revision as of 05:16, 27 January 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7qc9 is ON HOLD	+	The entry 7qc9 is ON HOLD until Paper Publication

	Authors: Beaumet, M., Dose, A., Braeuer, A., Mahy, J., Ghattas, W., Groll, M., Hess, C.		Authors: Beaumet, M., Dose, A., Braeuer, A., Mahy, J., Ghattas, W., Groll, M., Hess, C.

OCA: Protected "7qc9" [edit=sysop:move=sysop]

OCA — Wed, 01 Dec 2021 05:05:56 GMT

Protected "7qc9" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:05, 1 December 2021

OCA: New page: '''Unreleased structure''' The entry 7qc9 is ON HOLD Authors: Beaumet, M., Dose, A., Braeuer, A., Mahy, J., Ghattas, W., Groll, M., Hess, C. Description: HisF-C9A-D11E-V33A_L50H_I52H m...

OCA — Wed, 01 Dec 2021 05:05:55 GMT

New page: '''Unreleased structure''' The entry 7qc9 is ON HOLD Authors: Beaumet, M., Dose, A., Braeuer, A., Mahy, J., Ghattas, W., Groll, M., Hess, C. Description: HisF-C9A-D11E-V33A_L50H_I52H m...

New page

'''Unreleased structure'''

The entry 7qc9 is ON HOLD

Authors: Beaumet, M., Dose, A., Braeuer, A., Mahy, J., Ghattas, W., Groll, M., Hess, C.

Description: HisF-C9A-D11E-V33A_L50H_I52H mutant in complex with Ni(II) from T. maritima
[[Category: Unreleased Structures]]
[[Category: Hess, C]]
[[Category: Beaumet, M]]
[[Category: Groll, M]]
[[Category: Ghattas, W]]
[[Category: Braeuer, A]]
[[Category: Mahy, J]]
[[Category: Dose, A]]