7qcq - Revision history

OCA at 13:16, 1 February 2024

OCA — Thu, 01 Feb 2024 13:16:56 GMT

←Older revision		Revision as of 13:16, 1 February 2024
Line 1:		Line 1:

-	====	+	==VHH Z70 in interaction with PHF6 Tau peptide==
-	<StructureSection load='7qcq' size='340' side='right'caption='[[7qcq]]' scene=''>	+	<StructureSection load='7qcq' size='340' side='right'caption='[[7qcq]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7qcq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QCQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QCQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qcq OCA], [https://pdbe.org/7qcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qcq RCSB], [https://www.ebi.ac.uk/pdbsum/7qcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qcq ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qcq OCA], [https://pdbe.org/7qcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qcq RCSB], [https://www.ebi.ac.uk/pdbsum/7qcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qcq ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tau proteins aggregate into filaments in brain cells in Alzheimer's disease and related disorders referred to as tauopathies. Here, we used fragments of camelid heavy-chain-only antibodies (VHHs or single domain antibody fragments) targeting Tau as immuno-modulators of its pathologic seeding. A VHH issued from the screen against Tau of a synthetic phage-display library of humanized VHHs was selected for its capacity to bind Tau microtubule-binding domain, composing the core of Tau fibrils. This parent VHH was optimized to improve its biochemical properties and to act in the intra-cellular compartment, resulting in VHH Z70. VHH Z70 precisely binds the PHF6 sequence, known for its nucleation capacity, as shown by the crystal structure of the complex. VHH Z70 was more efficient than the parent VHH to inhibit in vitro Tau aggregation in heparin-induced assays. Expression of VHH Z70 in a cellular model of Tau seeding also decreased the aggregation-reporting fluorescence signal. Finally, intra-cellular expression of VHH Z70 in the brain of an established tauopathy mouse seeding model demonstrated its capacity to mitigate accumulation of pathological Tau. VHH Z70, by targeting Tau inside brain neurons, where most of the pathological Tau resides, provides an immunological tool to target the intra-cellular compartment in tauopathies.
		+
		+	Inhibition of Tau seeding by targeting Tau nucleation core within neurons with a single domain antibody fragment.,Danis C, Dupre E, Zejneli O, Caillierez R, Arrial A, Begard S, Mortelecque J, Eddarkaoui S, Loyens A, Cantrelle FX, Hanoulle X, Rain JC, Colin M, Buee L, Landrieu I Mol Ther. 2022 Apr 6;30(4):1484-1499. doi: 10.1016/j.ymthe.2022.01.009. Epub 2022 , Jan 7. PMID:35007758<ref>PMID:35007758</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7qcq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lama glama]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Arrial A]]
		+	[[Category: Begard S]]
		+	[[Category: Buee L]]
		+	[[Category: Caillierez R]]
		+	[[Category: Cantrelle F-X]]
		+	[[Category: Colin M]]
		+	[[Category: Danis C]]
		+	[[Category: Dupre E]]
		+	[[Category: Hanoulle X]]
		+	[[Category: Landrieu I]]
		+	[[Category: Loyens A]]
		+	[[Category: Mortelecque J]]
		+	[[Category: Rain J-C]]
		+	[[Category: Zejneli O]]

OCA at 09:58, 8 December 2021

OCA — Wed, 08 Dec 2021 09:58:08 GMT

←Older revision		Revision as of 09:58, 8 December 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7qcq ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7qcq' size='340' side='right'caption='[[7qcq]]' scene=''>
-	~~Authors~~: ~~Danis, C~~.~~, Dupre, E~~.~~, Zejneli, O., Caillierez, R., Arrial, A., Begard, S., Loyens, A., Mortelecque, J., Cantrelle, F~~.-X.~~, Hanoulle, X~~.~~, Rain, J~~.~~-C., Colin, M., Buee, L., Landrieu, I~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~VHH Z70 in interaction with PHF6 Tau peptide~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qcq OCA], [https://pdbe.org/7qcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qcq RCSB], [https://www.ebi.ac.uk/pdbsum/7qcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qcq ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	~~[[Category: Danis~~, ~~C]]~~	+	__TOC__
-	[~~[Category~~: ~~Cantrelle, F~~.-X]]	+	</StructureSection>
-	~~[[Category: Caillierez~~, ~~R]]~~	+	[[Category: Large Structures]]
-	[~~[Category~~: ~~Begard, S~~]]	+	[[Category: Z-disk]]
-	~~[[Category: Landrieu~~, ~~I]]~~	+
-	[~~[Category~~: ~~Arrial, A~~]]	+
-	~~[[Category: Loyens~~, ~~A]]~~	+
-	[~~[Category~~: ~~Colin, M~~]]	+
-	~~[[Category: Buee~~, ~~L]]~~	+
-	[~~[Category~~: ~~Zejneli, O]~~]	+
-	~~[[Category: Dupre, E]]~~	+
-	~~[[Category: Rain, J.-C]]~~	+
-	[[Category: ~~Mortelecque, J~~]]	+
-	[[Category: ~~Hanoulle, X~~]]	+

OCA: Protected "7qcq" [edit=sysop:move=sysop]

OCA — Wed, 01 Dec 2021 05:07:09 GMT

Protected "7qcq" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:07, 1 December 2021

OCA: New page: '''Unreleased structure''' The entry 7qcq is ON HOLD Authors: Danis, C., Dupre, E., Zejneli, O., Caillierez, R., Arrial, A., Begard, S., Loyens, A., Mortelecque, J., Cantrelle, F.-X., H...

OCA — Wed, 01 Dec 2021 05:07:08 GMT

New page: '''Unreleased structure''' The entry 7qcq is ON HOLD Authors: Danis, C., Dupre, E., Zejneli, O., Caillierez, R., Arrial, A., Begard, S., Loyens, A., Mortelecque, J., Cantrelle, F.-X., H...

New page

'''Unreleased structure'''

The entry 7qcq is ON HOLD

Authors: Danis, C., Dupre, E., Zejneli, O., Caillierez, R., Arrial, A., Begard, S., Loyens, A., Mortelecque, J., Cantrelle, F.-X., Hanoulle, X., Rain, J.-C., Colin, M., Buee, L., Landrieu, I.

Description: VHH Z70 in interaction with PHF6 Tau peptide
[[Category: Unreleased Structures]]
[[Category: Danis, C]]
[[Category: Cantrelle, F.-X]]
[[Category: Caillierez, R]]
[[Category: Begard, S]]
[[Category: Landrieu, I]]
[[Category: Arrial, A]]
[[Category: Loyens, A]]
[[Category: Colin, M]]
[[Category: Buee, L]]
[[Category: Zejneli, O]]
[[Category: Dupre, E]]
[[Category: Rain, J.-C]]
[[Category: Mortelecque, J]]
[[Category: Hanoulle, X]]