7qd0 - Revision history

OCA at 13:17, 1 February 2024

OCA — Thu, 01 Feb 2024 13:17:10 GMT

←Older revision		Revision as of 13:17, 1 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7qd0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Planktothrix_agardhii Planktothrix agardhii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QD0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7qd0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Planktothrix_agardhii Planktothrix agardhii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QD0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qd0 OCA], [https://pdbe.org/7qd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qd0 RCSB], [https://www.ebi.ac.uk/pdbsum/7qd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qd0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qd0 OCA], [https://pdbe.org/7qd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qd0 RCSB], [https://www.ebi.ac.uk/pdbsum/7qd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qd0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0A1J1JHR9_PLAAG A0A1J1JHR9_PLAAG]]	+	[https://www.uniprot.org/uniprot/A0A1J1JHR9_PLAAG A0A1J1JHR9_PLAAG]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:24, 8 September 2022

OCA — Thu, 08 Sep 2022 06:24:20 GMT

←Older revision		Revision as of 06:24, 8 September 2022
Line 3:		Line 3:
	<StructureSection load='7qd0' size='340' side='right'caption='[[7qd0]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='7qd0' size='340' side='right'caption='[[7qd0]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7qd0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QD0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7qd0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Planktothrix_agardhii Planktothrix agardhii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QD0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qd0 OCA], [https://pdbe.org/7qd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qd0 RCSB], [https://www.ebi.ac.uk/pdbsum/7qd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qd0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qd0 OCA], [https://pdbe.org/7qd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qd0 RCSB], [https://www.ebi.ac.uk/pdbsum/7qd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qd0 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[[https://www.uniprot.org/uniprot/A0A1J1JHR9_PLAAG A0A1J1JHR9_PLAAG]]
-	The orange carotenoid protein (OCP) is a photoactive protein involved in cyanobacterial photoprotection. Here, we report on the functional, spectral and structural characteristics of the peculiar Planktothrix PCC7805 OCP (Plankto-OCP). We show that this OCP variant is characterized by higher photoactivation and recovery rates, and a stronger energy-quenching activity, compared to other OCP studied thus far. We characterize the effect of the functionalizing carotenoid and of his-tagging on these reactions, and identify the time scales on which these modifications affect photoactivation. The presence of a his-tag at the C-terminus has a large influence on photoactivation, thermal recovery and PBS-fluorescence quenching, and likewise for the nature of the carotenoid that additionally affects the yield and characteristics of excited states and the ns-s dynamics of photoactivated OCP. By solving the structures of Plankto-OCP in the ECN- and CAN-functionalized states, each in two closely-related crystal forms, we further unveil the molecular breathing motions that animate Plankto-OCP at the monomer and dimer levels. We finally discuss the structural changes that could explain the peculiar properties of Plankto-OCP.	+
-		+
-	~~Structure-function-dynamics relationships in the peculiar Planktothrix PCC7805 OCP1~~: Impact of his-tagging and carotenoid type.,Wilson A, Andreeva EA, Nizinski SJ, Talbot L, Hartmann E, Schlichting I, Burdzinski G, Sliwa M, Kirilovsky D, Colletier JP Biochim Biophys Acta Bioenerg. 2022 Jun 23;1863(7):148584. doi:, 10.1016/~~j.bbabio.2022.148584. PMID:35752265<ref>PMID:35752265<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 7qd0" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Andreeva~~, E A~~]]	+	[[Category: Planktothrix agardhii]]
-	[[Category: Colletier, J P]]	+	[[Category: Andreeva EA]]
-	[[Category: Hartmann, E]]	+	[[Category: Colletier J-P]]
-	[[Category: Schlichting, I]]	+	[[Category: Hartmann E]]
-	~~[[Category: Carotenoid binding protein]]~~	+	[[Category: Schlichting I]]
-	~~[[Category: Carotenoid-binding]]~~	+
-	~~[[Category: Photoprotection]]~~	+
-	~~[[Category: Photosynthesis~~]]	+

OCA at 05:25, 13 July 2022

OCA — Wed, 13 Jul 2022 05:25:14 GMT

←Older revision		Revision as of 05:25, 13 July 2022
Line 1:		Line 1:

	==Structure of the orange carotenoid protein from Planktothrix agardhii binding echinenone in the C2 space group==		==Structure of the orange carotenoid protein from Planktothrix agardhii binding echinenone in the C2 space group==
-	<StructureSection load='7qd0' size='340' side='right'caption='[[7qd0]]' scene=''>	+	<StructureSection load='7qd0' size='340' side='right'caption='[[7qd0]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QD0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7qd0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QD0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qd0 OCA], [https://pdbe.org/7qd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qd0 RCSB], [https://www.ebi.ac.uk/pdbsum/7qd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qd0 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qd0 OCA], [https://pdbe.org/7qd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qd0 RCSB], [https://www.ebi.ac.uk/pdbsum/7qd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qd0 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The orange carotenoid protein (OCP) is a photoactive protein involved in cyanobacterial photoprotection. Here, we report on the functional, spectral and structural characteristics of the peculiar Planktothrix PCC7805 OCP (Plankto-OCP). We show that this OCP variant is characterized by higher photoactivation and recovery rates, and a stronger energy-quenching activity, compared to other OCP studied thus far. We characterize the effect of the functionalizing carotenoid and of his-tagging on these reactions, and identify the time scales on which these modifications affect photoactivation. The presence of a his-tag at the C-terminus has a large influence on photoactivation, thermal recovery and PBS-fluorescence quenching, and likewise for the nature of the carotenoid that additionally affects the yield and characteristics of excited states and the ns-s dynamics of photoactivated OCP. By solving the structures of Plankto-OCP in the ECN- and CAN-functionalized states, each in two closely-related crystal forms, we further unveil the molecular breathing motions that animate Plankto-OCP at the monomer and dimer levels. We finally discuss the structural changes that could explain the peculiar properties of Plankto-OCP.
		+
		+	Structure-function-dynamics relationships in the peculiar Planktothrix PCC7805 OCP1: Impact of his-tagging and carotenoid type.,Wilson A, Andreeva EA, Nizinski SJ, Talbot L, Hartmann E, Schlichting I, Burdzinski G, Sliwa M, Kirilovsky D, Colletier JP Biochim Biophys Acta Bioenerg. 2022 Jun 23;1863(7):148584. doi:, 10.1016/j.bbabio.2022.148584. PMID:35752265<ref>PMID:35752265</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7qd0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Andreeva EA]]	+	[[Category: Andreeva, E A]]
-	[[Category: Colletier J-P]]	+	[[Category: Colletier, J P]]
-	[[Category: Hartmann E]]	+	[[Category: Hartmann, E]]
-	[[Category: Schlichting I]]	+	[[Category: Schlichting, I]]
		+	[[Category: Carotenoid binding protein]]
		+	[[Category: Carotenoid-binding]]
		+	[[Category: Photoprotection]]
		+	[[Category: Photosynthesis]]

OCA at 16:12, 6 July 2022

OCA — Wed, 06 Jul 2022 16:12:47 GMT

←Older revision		Revision as of 16:12, 6 July 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7qd0 ~~is ON HOLD until Paper Publication~~	+	==Structure of the orange carotenoid protein from Planktothrix agardhii binding echinenone in the C2 space group==
-		+	<StructureSection load='7qd0' size='340' side='right'caption='[[7qd0]]' scene=''>
-	~~Authors~~: ~~Andreeva~~, E.A., ~~Hartmann~~, E., ~~Schlichting, I~~., ~~Colletier, J~~.-P.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QD0 FirstGlance]. <br>
-	~~Description: Structure of the orange carotenoid protein from Planktothrix agardhii binding echinenone in the C2 space group~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qd0 OCA], [https://pdbe.org/7qd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qd0 RCSB], [https://www.ebi.ac.uk/pdbsum/7qd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qd0 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Colletier, J.-P~~]]	+	__TOC__
-	[[Category: ~~Schlichting, I~~]]	+	</StructureSection>
-	[[Category: Hartmann, E]]	+	[[Category: Large Structures]]
-	[[Category: ~~Andreeva, E.A~~]]	+	[[Category: Andreeva EA]]
		+	[[Category: Colletier J-P]]
		+	[[Category: Hartmann E]]
		+	[[Category: Schlichting I]]

OCA at 11:47, 29 December 2021

OCA — Wed, 29 Dec 2021 11:47:53 GMT

←Older revision		Revision as of 11:47, 29 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7qd0 is ON HOLD	+	The entry 7qd0 is ON HOLD until Paper Publication

	Authors: Andreeva, E.A., Hartmann, E., Schlichting, I., Colletier, J.-P.		Authors: Andreeva, E.A., Hartmann, E., Schlichting, I., Colletier, J.-P.

OCA: Protected "7qd0" [edit=sysop:move=sysop]

OCA — Wed, 01 Dec 2021 05:08:10 GMT

Protected "7qd0" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:08, 1 December 2021

OCA: New page: '''Unreleased structure''' The entry 7qd0 is ON HOLD Authors: Andreeva, E.A., Hartmann, E., Schlichting, I., Colletier, J.-P. Description: Structure of the orange carotenoid protein fr...

OCA — Wed, 01 Dec 2021 05:08:09 GMT

New page: '''Unreleased structure''' The entry 7qd0 is ON HOLD Authors: Andreeva, E.A., Hartmann, E., Schlichting, I., Colletier, J.-P. Description: Structure of the orange carotenoid protein fr...

New page

'''Unreleased structure'''

The entry 7qd0 is ON HOLD

Authors: Andreeva, E.A., Hartmann, E., Schlichting, I., Colletier, J.-P.

Description: Structure of the orange carotenoid protein from Planktothrix agardhii binding echinenone in the C2 space group
[[Category: Unreleased Structures]]
[[Category: Colletier, J.-P]]
[[Category: Schlichting, I]]
[[Category: Hartmann, E]]
[[Category: Andreeva, E.A]]