7qfz - Revision history

OCA at 07:53, 1 May 2024

OCA — Wed, 01 May 2024 07:53:36 GMT

←Older revision		Revision as of 07:53, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='7qfz' size='340' side='right'caption='[[7qfz]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='7qfz' size='340' side='right'caption='[[7qfz]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7qfz]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7qfz]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_fergusonii_ATCC_35469 Escherichia fergusonii ATCC 35469]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QFZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qfz OCA], [https://pdbe.org/7qfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qfz RCSB], [https://www.ebi.ac.uk/pdbsum/7qfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qfz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qfz OCA], [https://pdbe.org/7qfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qfz RCSB], [https://www.ebi.ac.uk/pdbsum/7qfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qfz ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/B7L3Y3_ESCF3 B7L3Y3_ESCF3]
-	Bacteria are under constant assault by bacteriophages and other mobile genetic elements. As a result, bacteria have evolved a multitude of systems that protect from attack. Genes encoding bacterial defence mechanisms can be clustered into 'defence islands', providing a potentially synergistic level of protection against a wider range of assailants. However, there is a comparative paucity of information on how expression of these defence systems is controlled. Here, we functionally characterize a transcriptional regulator, BrxR, encoded within a recently described phage defence island from a multidrug resistant plasmid of the emerging pathogen Escherichia fergusonii. Using a combination of reporters and electrophoretic mobility shift assays, we discovered that BrxR acts as a repressor. We present the structure of BrxR to 2.15 A, the first structure of this family of transcription factors, and pinpoint a likely binding site for ligands within the WYL-domain. Bioinformatic analyses demonstrated that BrxR-family homologues are widespread amongst bacteria. About half (48%) of identified BrxR homologues were co-localized with a diverse array of known phage defence systems, either alone or clustered into defence islands. BrxR is a novel regulator that reveals a common mechanism for controlling the expression of the bacterial phage defence arsenal.	+
-		+
-	A widespread family of WYL-domain transcriptional regulators co-localizes with diverse phage defence systems and islands.,Picton DM, Harling-Lee JD, Duffner SJ, Went SC, Morgan RD, Hinton JCD, Blower TR Nucleic Acids Res. 2022 May 20;50(9):~~5191-5207. doi: 10.1093~~/~~nar~~/~~gkac334~~. ~~PMID:35544231<ref>PMID:35544231</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.~~S. National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 7qfz" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia fergusonii ATCC 35469]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Blower~~, T R~~]]	+	[[Category: Blower TR]]
-	[[Category: Picton~~, D M]]~~	+	[[Category: Picton DM]]
-	~~[[Category: Dna binding]]~~	+
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Phage defence]]~~	+
-	~~[[Category: Transcription factor~~]]	+

OCA at 08:14, 25 May 2022

OCA — Wed, 25 May 2022 08:14:34 GMT

←Older revision		Revision as of 08:14, 25 May 2022
Line 1:		Line 1:

	==BrxR, a WYL-domain containing transcriptional regulator==		==BrxR, a WYL-domain containing transcriptional regulator==
-	<StructureSection load='7qfz' size='340' side='right'caption='[[7qfz]]' scene=''>	+	<StructureSection load='7qfz' size='340' side='right'caption='[[7qfz]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7qfz]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QFZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qfz OCA], [https://pdbe.org/7qfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qfz RCSB], [https://www.ebi.ac.uk/pdbsum/7qfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qfz ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qfz OCA], [https://pdbe.org/7qfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qfz RCSB], [https://www.ebi.ac.uk/pdbsum/7qfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qfz ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteria are under constant assault by bacteriophages and other mobile genetic elements. As a result, bacteria have evolved a multitude of systems that protect from attack. Genes encoding bacterial defence mechanisms can be clustered into 'defence islands', providing a potentially synergistic level of protection against a wider range of assailants. However, there is a comparative paucity of information on how expression of these defence systems is controlled. Here, we functionally characterize a transcriptional regulator, BrxR, encoded within a recently described phage defence island from a multidrug resistant plasmid of the emerging pathogen Escherichia fergusonii. Using a combination of reporters and electrophoretic mobility shift assays, we discovered that BrxR acts as a repressor. We present the structure of BrxR to 2.15 A, the first structure of this family of transcription factors, and pinpoint a likely binding site for ligands within the WYL-domain. Bioinformatic analyses demonstrated that BrxR-family homologues are widespread amongst bacteria. About half (48%) of identified BrxR homologues were co-localized with a diverse array of known phage defence systems, either alone or clustered into defence islands. BrxR is a novel regulator that reveals a common mechanism for controlling the expression of the bacterial phage defence arsenal.
		+
		+	A widespread family of WYL-domain transcriptional regulators co-localizes with diverse phage defence systems and islands.,Picton DM, Harling-Lee JD, Duffner SJ, Went SC, Morgan RD, Hinton JCD, Blower TR Nucleic Acids Res. 2022 May 20;50(9):5191-5207. doi: 10.1093/nar/gkac334. PMID:35544231<ref>PMID:35544231</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7qfz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Blower TR]]	+	[[Category: Blower, T R]]
-	[[Category: Picton DM]]	+	[[Category: Picton, D M]]
		+	[[Category: Dna binding]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Phage defence]]
		+	[[Category: Transcription factor]]

OCA at 10:15, 18 May 2022

OCA — Wed, 18 May 2022 10:15:47 GMT

←Older revision		Revision as of 10:15, 18 May 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7qfz ~~is ON HOLD until Paper Publication~~	+	==BrxR, a WYL-domain containing transcriptional regulator==
-		+	<StructureSection load='7qfz' size='340' side='right'caption='[[7qfz]]' scene=''>
-	~~Authors~~: ~~Picton~~, D.M., ~~Blower~~, T.R.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QFZ FirstGlance]. <br>
-	~~Description: Phage defence protein~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qfz OCA], [https://pdbe.org/7qfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qfz RCSB], [https://www.ebi.ac.uk/pdbsum/7qfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qfz ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Picton, D.M~~]]	+	__TOC__
-	[[Category: ~~Blower, T.R~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Blower TR]]
		+	[[Category: Picton DM]]

OCA at 13:53, 22 December 2021

OCA — Wed, 22 Dec 2021 13:53:55 GMT

←Older revision		Revision as of 13:53, 22 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7qfz is ON HOLD	+	The entry 7qfz is ON HOLD until Paper Publication

	Authors: Picton, D.M., Blower, T.R.		Authors: Picton, D.M., Blower, T.R.

OCA: Protected "7qfz" [edit=sysop:move=sysop]

OCA — Wed, 15 Dec 2021 15:35:09 GMT

Protected "7qfz" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:35, 15 December 2021

OCA: New page: '''Unreleased structure''' The entry 7qfz is ON HOLD Authors: Picton, D.M., Blower, T.R. Description: Phage defence protein Category: Unreleased Structures [[Category: Picton, D.M]...

OCA — Wed, 15 Dec 2021 15:35:07 GMT

New page: '''Unreleased structure''' The entry 7qfz is ON HOLD Authors: Picton, D.M., Blower, T.R. Description: Phage defence protein Category: Unreleased Structures [[Category: Picton, D.M]...

New page

'''Unreleased structure'''

The entry 7qfz is ON HOLD

Authors: Picton, D.M., Blower, T.R.

Description: Phage defence protein
[[Category: Unreleased Structures]]
[[Category: Picton, D.M]]
[[Category: Blower, T.R]]