7qp8 - Revision history

OCA at 13:20, 1 February 2024

OCA — Thu, 01 Feb 2024 13:20:44 GMT

←Older revision		Revision as of 13:20, 1 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7qp8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_sp._G15-21 Vibrio sp. G15-21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QP8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7qp8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_sp._G15-21 Vibrio sp. G15-21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QP8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qp8 OCA], [https://pdbe.org/7qp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qp8 RCSB], [https://www.ebi.ac.uk/pdbsum/7qp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qp8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qp8 OCA], [https://pdbe.org/7qp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qp8 RCSB], [https://www.ebi.ac.uk/pdbsum/7qp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qp8 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:12, 3 November 2022

OCA — Thu, 03 Nov 2022 07:12:49 GMT

←Older revision		Revision as of 07:12, 3 November 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7qp8 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of Vibrio alkaline phosphatase with bound HEPES==
		+	<StructureSection load='7qp8' size='340' side='right'caption='[[7qp8]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7qp8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_sp._G15-21 Vibrio sp. G15-21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QP8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qp8 OCA], [https://pdbe.org/7qp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qp8 RCSB], [https://www.ebi.ac.uk/pdbsum/7qp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qp8 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q93P54_9VIBR Q93P54_9VIBR]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enzyme stability and function can be affected by various environmental factors, such as temperature, pH, and ionic strength. Enzymes that are located outside the relatively unchanging environment of the cytosol, such as those residing in the periplasmic space of bacteria or extracellularly secreted, are challenged by more fluctuations in the aqueous medium. Bacterial alkaline phosphatases (APs) are generally affected by ionic strength of the medium, but this varies substantially between species. An AP from the marine bacterium Vibrio splendidus (VAP) shows complex pH-dependent activation and stabilization in the 0-1.0 M range of halogen salts and has been hypothesized to specifically bind chloride anions. Here, using X-ray crystallography and anomalous scattering, we have located two chloride binding sites in the structure of VAP, one in the active site and another one at a peripheral site. Further characterization of the binding sites using site-directed mutagenesis and small-angle X-ray scattering showed that upon binding of chloride to the peripheral site, structural dynamics decreased locally, resulting in thermal stabilization of the VAP active conformation. Binding of the chloride ion in the active site did not displace the bound inorganic phosphate product, but it may promote product release by facilitating rotational stabilization of the substrate-binding Arg129. Overall, these results reveal the complex nature and dynamics of chloride binding to enzymes through long-range modulation of electronic potential in the vicinity of the active site, resulting in increased catalytic efficiency and stability.

-	~~Authors: Markusson~~, S., Hjorleifsson~~, J.G.~~, Kursula, P., Asgeirsson, B.	+	Structural Characterization of Functionally Important Chloride Binding Sites in the Marine Vibrio Alkaline Phosphatase.,Markusson S, Hjorleifsson JG, Kursula P, Asgeirsson B Biochemistry. 2022 Oct 18;61(20):2248-2260. doi: 10.1021/acs.biochem.2c00438., Epub 2022 Oct 4. PMID:36194497<ref>PMID:36194497</ref>

-	~~Description: Crystal structure~~ of ~~Vibrio alkaline phosphatase with bound HEPES~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Asgeirsson, B]]	+	<div class="pdbe-citations 7qp8" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Kursula, P~~]]	+	== References ==
-	[[Category: ~~Hjorleifsson, J.G~~]]	+	<references/>
-	[[Category: Markusson, S]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Vibrio sp. G15-21]]
		+	[[Category: Asgeirsson B]]
		+	[[Category: Hjorleifsson JG]]
		+	[[Category: Kursula P]]
		+	[[Category: Markusson S]]

OCA at 06:05, 9 February 2022

OCA — Wed, 09 Feb 2022 06:05:36 GMT

←Older revision		Revision as of 06:05, 9 February 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7qp8 is ON HOLD	+	The entry 7qp8 is ON HOLD until Paper Publication

	Authors: Markusson, S., Hjorleifsson, J.G., Kursula, P., Asgeirsson, B.		Authors: Markusson, S., Hjorleifsson, J.G., Kursula, P., Asgeirsson, B.

OCA: Protected "7qp8" [edit=sysop:move=sysop]

OCA — Wed, 12 Jan 2022 08:30:39 GMT

Protected "7qp8" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:30, 12 January 2022

OCA: New page: '''Unreleased structure''' The entry 7qp8 is ON HOLD Authors: Markusson, S., Hjorleifsson, J.G., Kursula, P., Asgeirsson, B. Description: Crystal structure of Vibrio alkaline phosphata...

OCA — Wed, 12 Jan 2022 08:30:38 GMT

New page: '''Unreleased structure''' The entry 7qp8 is ON HOLD Authors: Markusson, S., Hjorleifsson, J.G., Kursula, P., Asgeirsson, B. Description: Crystal structure of Vibrio alkaline phosphata...

New page

'''Unreleased structure'''

The entry 7qp8 is ON HOLD

Authors: Markusson, S., Hjorleifsson, J.G., Kursula, P., Asgeirsson, B.

Description: Crystal structure of Vibrio alkaline phosphatase with bound HEPES
[[Category: Unreleased Structures]]
[[Category: Asgeirsson, B]]
[[Category: Kursula, P]]
[[Category: Hjorleifsson, J.G]]
[[Category: Markusson, S]]