7qq1 - Revision history

OCA at 13:21, 1 February 2024

OCA — Thu, 01 Feb 2024 13:21:04 GMT

←Older revision		Revision as of 13:21, 1 February 2024
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	<StructureSection load='7qq1' size='340' side='right'caption='[[7qq1]], [[Resolution\|resolution]] 1.48Å' scene=''>		<StructureSection load='7qq1' size='340' side='right'caption='[[7qq1]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7qq1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QQ1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7qq1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QQ1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=RH:RHODIUM'>RH</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.48Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=RH:RHODIUM'>RH</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qq1 OCA], [https://pdbe.org/7qq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qq1 RCSB], [https://www.ebi.ac.uk/pdbsum/7qq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qq1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qq1 OCA], [https://pdbe.org/7qq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qq1 RCSB], [https://www.ebi.ac.uk/pdbsum/7qq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qq1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>	+	[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 7qq1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7qq1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Lysozyme]]~~	+	[[Category: Loreto D]]
-	[[Category: Loreto, D]]	+	[[Category: Merlino A]]
-	[[Category: Merlino, A]]	+
-	~~[[Category: Dimetallic paddlewheel complex]]~~	+
-	~~[[Category: Dirhodium tetraacetate derivative]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protein metalation~~]]	+

OCA at 07:25, 9 March 2022

OCA — Wed, 09 Mar 2022 07:25:23 GMT

←Older revision		Revision as of 07:25, 9 March 2022
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	==X-ray structure of the adduct obtained upon reaction of [cis-Rh2(OCOCH3)2(OCOCF3)2] with HEWL==		==X-ray structure of the adduct obtained upon reaction of [cis-Rh2(OCOCH3)2(OCOCF3)2] with HEWL==
-	<StructureSection load='7qq1' size='340' side='right'caption='[[7qq1]]' scene=''>	+	<StructureSection load='7qq1' size='340' side='right'caption='[[7qq1]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QQ1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7qq1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QQ1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qq1 OCA], [https://pdbe.org/7qq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qq1 RCSB], [https://www.ebi.ac.uk/pdbsum/7qq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qq1 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=RH:RHODIUM'>RH</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qq1 OCA], [https://pdbe.org/7qq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qq1 RCSB], [https://www.ebi.ac.uk/pdbsum/7qq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qq1 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dirhodium complexes of general formula [Rh2(O2CR)4]L2 are a well-known class of bimetallic compounds that are used as efficient catalysts for a variety of reactions and have been shown to be potent antibacterial and anticancer agents. The catalytic and biological properties of these complexes largely depend on the nature of the bridging carboxylate ligands. Trifluoroacetate (tfa)-containing dirhodium compounds have been used to build artificial metalloenzymes upon reaction with peptides and have been shown to be more cytotoxic than dirhodium tetraacetate. However, there is no structural information on the interaction between these compounds and proteins. Here, cis-Rh2(mu-O2CCH3)2(mu-O2CCF3)2 ([cis-Rh2(OAc)2(tfa)2]) has been synthesized and its reaction with bovine pancreatic ribonuclease (RNase A) and hen egg white lysozyme (HEWL) was analyzed using a combination of different techniques, including Fluorine-19 nuclear magnetic resonance spectroscopy and macromolecular X-ray crystallography, with the aim to unveil the differences in the reactivity of tfa-containing dihrodium complexes with proteins when compared to [Rh2(OAc)4]. [cis-Rh2(OAc)2(tfa)2] and [Rh2(OAc)4] bind the N atoms of His side chains of RNase A at the axial position; however the fluorine-containing compound rapidly loses its tfa ligands, while [Rh2(OAc)4] can retain the acetate ligands upon protein binding. The reactivity of [cis-Rh2(OAc)2(tfa)2] with HEWL is slightly distinct when compared to that of [Rh2(OAc)4] under the same experimental conditions; however, both [cis-Rh2(OAc)2(tfa)2] and [Rh2(OAc)4] degrade when soaked within HEWL crystals. These results provide a structural-based guide for the design of new heterogenous chiral dirhodium/peptide and dirhodium/protein adducts with application in the fields of organic synthesis and asymmetric catalysis.
		+
		+	Reactivity of a fluorine-containing dirhodium tetracarboxylate compound with proteins.,Loreto D, Esposito A, Demitri N, Guaragna A, Merlino A Dalton Trans. 2022 Mar 1;51(9):3695-3705. doi: 10.1039/d2dt00082b. PMID:35166290<ref>PMID:35166290</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7qq1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Loreto D]]	+	[[Category: Lysozyme]]
-	[[Category: Merlino A]]	+	[[Category: Loreto, D]]
		+	[[Category: Merlino, A]]
		+	[[Category: Dimetallic paddlewheel complex]]
		+	[[Category: Dirhodium tetraacetate derivative]]
		+	[[Category: Hydrolase]]
		+	[[Category: Protein metalation]]

OCA at 06:20, 2 March 2022

OCA — Wed, 02 Mar 2022 06:20:45 GMT

←Older revision		Revision as of 06:20, 2 March 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7qq1 is ON HOLD until Paper Publication~~	+	==X-ray structure of the adduct obtained upon reaction of [cis-Rh2(OCOCH3)2(OCOCF3)2] with HEWL==
-		+	<StructureSection load='7qq1' size='340' side='right'caption='[[7qq1]]' scene=''>
-	~~Authors: Loreto, D., Merlino, A.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QQ1 FirstGlance]. <br>
-	~~Description:~~ X-ray structure of the adduct obtained upon reaction of [cis-Rh2(OCOCH3)2(OCOCF3)2] with HEWL	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qq1 OCA], [https://pdbe.org/7qq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qq1 RCSB], [https://www.ebi.ac.uk/pdbsum/7qq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qq1 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Loreto, D]]	+	__TOC__
-	[[Category: Merlino, A]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Loreto D]]
		+	[[Category: Merlino A]]

OCA at 06:05, 9 February 2022

OCA — Wed, 09 Feb 2022 06:05:51 GMT

←Older revision		Revision as of 06:05, 9 February 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7qq1 is ON HOLD	+	The entry 7qq1 is ON HOLD until Paper Publication

	Authors: Loreto, D., Merlino, A.		Authors: Loreto, D., Merlino, A.

OCA: Protected "7qq1" [edit=sysop:move=sysop]

OCA — Wed, 12 Jan 2022 08:32:24 GMT

Protected "7qq1" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:32, 12 January 2022

OCA: New page: '''Unreleased structure''' The entry 7qq1 is ON HOLD Authors: Loreto, D., Merlino, A. Description: X-ray structure of the adduct obtained upon reaction of [cis-Rh2(OCOCH3)2(OCOCF3)2] w...

OCA — Wed, 12 Jan 2022 08:32:23 GMT

New page: '''Unreleased structure''' The entry 7qq1 is ON HOLD Authors: Loreto, D., Merlino, A. Description: X-ray structure of the adduct obtained upon reaction of [cis-Rh2(OCOCH3)2(OCOCF3)2] w...

New page

'''Unreleased structure'''

The entry 7qq1 is ON HOLD

Authors: Loreto, D., Merlino, A.

Description: X-ray structure of the adduct obtained upon reaction of [cis-Rh2(OCOCH3)2(OCOCF3)2] with HEWL
[[Category: Unreleased Structures]]
[[Category: Loreto, D]]
[[Category: Merlino, A]]