OCA at 04:42, 8 September 2022

OCA — Thu, 08 Sep 2022 04:42:35 GMT

←Older revision		Revision as of 04:42, 8 September 2022
Line 1:		Line 1:

-	====	+	==2.8 Angstrom cryo-EM structure of the dimeric cytochrome b6f-PetP complex from Synechocystis sp. PCC 6803 with natively bound lipids and plastoquinone molecules==
-	<StructureSection load='7r0w' size='340' side='right'caption='[[7r0w]]' scene=''>	+	<StructureSection load='7r0w' size='340' side='right'caption='[[7r0w]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7r0w]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis Synechocystis] and [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R0W FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r0w OCA], [https://pdbe.org/7r0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r0w RCSB], [https://www.ebi.ac.uk/pdbsum/7r0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r0w ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2WA:(1S,8E)-1-{[(2S)-1-HYDROXY-3-{[(1S)-1-HYDROXYPENTADECYL]OXY}PROPAN-2-YL]OXY}HEPTADEC-8-EN-1-OL'>2WA</scene>, <scene name='pdbligand=6PL:(4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM+4-OXIDE'>6PL</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=LMG:1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE'>LMG</scene>, <scene name='pdbligand=PGV:(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL+(11E)-OCTADEC-11-ENOATE'>PGV</scene>, <scene name='pdbligand=PL9:2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE'>PL9</scene>, <scene name='pdbligand=SQD:1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL'>SQD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r0w OCA], [https://pdbe.org/7r0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r0w RCSB], [https://www.ebi.ac.uk/pdbsum/7r0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r0w ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/PETM_SYNY3 PETM_SYNY3]] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In oxygenic photosynthesis, the cytochrome b6f (cytb6f) complex links the linear electron transfer (LET) reactions occurring at photosystems I and II and generates a transmembrane proton gradient via the Q-cycle. In addition to this central role in LET, cytb6f also participates in a range of processes including cyclic electron transfer (CET), state transitions and photosynthetic control. Many of the regulatory roles of cytb6f are facilitated by auxiliary proteins that differ depending upon the species, yet because of their weak and transient nature the structural details of these interactions remain unknown. An apparent key player in the regulatory balance between LET and CET in cyanobacteria is PetP, a approximately 10 kDa protein that is also found in red algae but not in green algae and plants. Here, we used cryogenic electron microscopy to determine the structure of the Synechocystis sp. PCC 6803 cytb6f complex in the presence and absence of PetP. Our structures show that PetP interacts with the cytoplasmic side of cytb6f, displacing the C-terminus of the PetG subunit and shielding the C-terminus of cytochrome b6, which binds the heme cn cofactor that is suggested to mediate CET. The structures also highlight key differences in the mode of plastoquinone binding between cyanobacterial and plant cytb6f complexes, which we suggest may reflect the unique combination of photosynthetic and respiratory electron transfer in cyanobacterial thylakoid membranes. The structure of cytb6f from a model cyanobacterial species amenable to genetic engineering will enhance future site-directed mutagenesis studies of structure-function relationships in this crucial ET complex.
		+
		+	Cryo-EM structures of the Synechocystis sp. PCC 6803 cytochrome b6f complex with and without the regulatory PetP subunit.,Proctor MS, Malone LA, Farmer DA, Swainsbury DJK, Hawkings FR, Pastorelli F, Emrich-Mills TZ, Siebert CA, Hunter CN, Johnson MP, Hitchcock A Biochem J. 2022 Jul 15;479(13):1487-1503. doi: 10.1042/BCJ20220124. PMID:35726684<ref>PMID:35726684</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7r0w" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synechocystis]]
		+	[[Category: Synechocystis sp. PCC 6803]]
		+	[[Category: Farmer DF]]
		+	[[Category: Hawkings FR]]
		+	[[Category: Hitchcock A]]
		+	[[Category: Johnson MP]]
		+	[[Category: Malone LA]]
		+	[[Category: Proctor MS]]
		+	[[Category: Swainsbury DPK]]

OCA at 16:13, 6 July 2022

OCA — Wed, 06 Jul 2022 16:13:07 GMT

←Older revision		Revision as of 16:13, 6 July 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7r0w ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7r0w' size='340' side='right'caption='[[7r0w]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r0w OCA], [https://pdbe.org/7r0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r0w RCSB], [https://www.ebi.ac.uk/pdbsum/7r0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r0w ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 04:59, 2 March 2022

OCA — Wed, 02 Mar 2022 04:59:50 GMT

←Older revision		Revision as of 04:59, 2 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7r0w is ON HOLD ~~until sometime in the future~~	+	The entry 7r0w is ON HOLD

	Authors:		Authors:

OCA at 06:26, 23 February 2022

OCA — Wed, 23 Feb 2022 06:26:00 GMT

←Older revision		Revision as of 06:26, 23 February 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7r0w is ON HOLD	+	The entry 7r0w is ON HOLD until sometime in the future

	Authors:		Authors:

OCA: Protected "7r0w" [edit=sysop:move=sysop]

OCA — Wed, 09 Feb 2022 06:12:13 GMT

Protected "7r0w" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:12, 9 February 2022

OCA: New page: '''Unreleased structure''' The entry 7r0w is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 09 Feb 2022 06:12:12 GMT

New page: '''Unreleased structure''' The entry 7r0w is ON HOLD Authors: Description: Category: Unreleased Structures

New page