7r3p - Revision history

OCA at 13:25, 1 February 2024

OCA — Thu, 01 Feb 2024 13:25:49 GMT

←Older revision		Revision as of 13:25, 1 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7r3p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rubrivivax_gelatinosus Rubrivivax gelatinosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R3P FirstGlance]. <br>		<table><tr><td colspan='2'>[[7r3p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rubrivivax_gelatinosus Rubrivivax gelatinosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R3P FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r3p OCA], [https://pdbe.org/7r3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r3p RCSB], [https://www.ebi.ac.uk/pdbsum/7r3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r3p ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r3p OCA], [https://pdbe.org/7r3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r3p RCSB], [https://www.ebi.ac.uk/pdbsum/7r3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r3p ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:25, 26 October 2022

OCA — Wed, 26 Oct 2022 06:25:13 GMT

←Older revision		Revision as of 06:25, 26 October 2022
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	==Apoform of the periplasmic FtrA/P19 protein from Rubrivivax gelatinosus (His-tag)==		==Apoform of the periplasmic FtrA/P19 protein from Rubrivivax gelatinosus (His-tag)==
-	<StructureSection load='7r3p' size='340' side='right'caption='[[7r3p]]' scene=''>	+	<StructureSection load='7r3p' size='340' side='right'caption='[[7r3p]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R3P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7r3p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rubrivivax_gelatinosus Rubrivivax gelatinosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R3P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r3p OCA], [https://pdbe.org/7r3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r3p RCSB], [https://www.ebi.ac.uk/pdbsum/7r3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r3p ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r3p OCA], [https://pdbe.org/7r3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r3p RCSB], [https://www.ebi.ac.uk/pdbsum/7r3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r3p ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A4R2M5S2_RUBGE A0A4R2M5S2_RUBGE]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Iron is an essential nutrient in bacteria. Its ferrous form, mostly present in low oxygen and acidic pH environments, can be imported using the specific Ftr-type transport system, which encompasses the conserved FtrABCD system found in pathogenic bacteria such as Bordetella, Brucella and Burkholderia. The non-pathogenicity and versatile metabolism of Rubrivivax gelatinosus make it an ideal model to study the FtrABCD system. Here, we report a new aspect of its regulation and the role of the periplasmic proteins FtrA and FtrB using in vivo and in vitro approaches. We investigated the metal binding mode and redox state of copper and iron to FtrA by crystallography and biophysical methods. An 'as isolated' FtrA protein from the bacterial periplasm contained a copper ion (Cu(+) ) identified by electron paramagnetic resonance (EPR). Copper is coordinated by four conserved side chains (His and Met) in the primary metal site. Structural analysis of R. gelatinosus FtrA and FtrA homologues revealed that copper binding induces a rearrangement of the His95 imidazole ring, releasing thereafter space, as well as both Asp45 and Asp92 side chains, for iron binding in the secondary metal site. EPR highlighted that FtrA can oxidize the bound ferrous ion into the ferric form by reducing the bound Cu(2+) into Cu(+) , both metal sites being separated by 7 A. Finally, we showed that FtrB binds iron and not copper. These results provide new insights into the mechanism of ferrous iron utilization by the conserved FtrABCD iron transporter for which we propose a new functional model.
		+
		+	New insights into the mechanism of iron transport through the bacterial Ftr-system present in pathogens.,Soisig Steunou A, Vigouroux A, Aumont-Nicaise M, Plancqueel S, Boussac A, Ouchane S, Morera S FEBS J. 2022 May 8. doi: 10.1111/febs.16476. PMID:35527501<ref>PMID:35527501</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7r3p" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Rubrivivax gelatinosus]]
	[[Category: Morera S]]		[[Category: Morera S]]
	[[Category: Vigouroux A]]		[[Category: Vigouroux A]]

OCA at 10:16, 18 May 2022

OCA — Wed, 18 May 2022 10:16:02 GMT

←Older revision		Revision as of 10:16, 18 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7r3p ~~is ON HOLD until Paper Publication~~	+	==Apoform of the periplasmic FtrA/P19 protein from Rubrivivax gelatinosus (His-tag)==
-		+	<StructureSection load='7r3p' size='340' side='right'caption='[[7r3p]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R3P FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r3p OCA], [https://pdbe.org/7r3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r3p RCSB], [https://www.ebi.ac.uk/pdbsum/7r3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r3p ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Morera S]]
		+	[[Category: Vigouroux A]]

OCA at 05:00, 2 March 2022

OCA — Wed, 02 Mar 2022 05:00:23 GMT

←Older revision		Revision as of 05:00, 2 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7r3p is ON HOLD	+	The entry 7r3p is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7r3p" [edit=sysop:move=sysop]

OCA — Wed, 16 Feb 2022 09:01:50 GMT

Protected "7r3p" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:01, 16 February 2022

OCA: New page: '''Unreleased structure''' The entry 7r3p is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 16 Feb 2022 09:01:49 GMT

New page: '''Unreleased structure''' The entry 7r3p is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7r3p is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]