OCA at 06:40, 8 February 2023

OCA — Wed, 08 Feb 2023 06:40:51 GMT

←Older revision		Revision as of 06:40, 8 February 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7r47 is ~~ON HOLD until Paper~~ Publication	+	==Bovine complex I in the presence of IM1761092, deactive class iii (Composite map)==
		+	<StructureSection load='7r47' size='340' side='right'caption='[[7r47]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7r47]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R47 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R47 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2MR:N3,+N4-DIMETHYLARGININE'>2MR</scene>, <scene name='pdbligand=3PE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>3PE</scene>, <scene name='pdbligand=AME:N-ACETYLMETHIONINE'>AME</scene>, <scene name='pdbligand=AYA:N-ACETYLALANINE'>AYA</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=EHZ:~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl]+(3~{S})-3-oxidanyltetradecanethioate'>EHZ</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=I49:1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine'>I49</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PC1:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PC1</scene>, <scene name='pdbligand=SAC:N-ACETYL-SERINE'>SAC</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r47 OCA], [https://pdbe.org/7r47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r47 RCSB], [https://www.ebi.ac.uk/pdbsum/7r47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r47 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q7JAS9_BOVIN Q7JAS9_BOVIN] Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity of complex I.[ARBA:ARBA00024297][RuleBase:RU003640]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The molecular mode of action of biguanides, including the drug metformin, which is widely used in the treatment of diabetes, is incompletely characterized. Here, we define the inhibitory drug-target interaction(s) of a model biguanide with mammalian respiratory complex I by combining cryo-electron microscopy and enzyme kinetics. We interpret these data to explain the selectivity of biguanide binding to different enzyme states. The primary inhibitory site is in an amphipathic region of the quinone-binding channel, and an additional binding site is in a pocket on the intermembrane-space side of the enzyme. An independent local chaotropic interaction, not previously described for any drug, displaces a portion of a key helix in the membrane domain. Our data provide a structural basis for biguanide action and enable the rational design of medicinal biguanides.

-	~~Authors~~:	+	Structural basis of mammalian respiratory complex I inhibition by medicinal biguanides.,Bridges HR, Blaza JN, Yin Z, Chung I, Pollak MN, Hirst J Science. 2023 Jan 27;379(6630):351-357. doi: 10.1126/science.ade3332. Epub 2023 , Jan 26. PMID:36701435<ref>PMID:36701435</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7r47" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bos taurus]]
		+	[[Category: Large Structures]]
		+	[[Category: Blaza JN]]
		+	[[Category: Bridges HR]]
		+	[[Category: Chung I]]
		+	[[Category: Hirst J]]
		+	[[Category: Yin Z]]

OCA at 04:58, 16 March 2022

OCA — Wed, 16 Mar 2022 04:58:43 GMT

←Older revision		Revision as of 04:58, 16 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7r47 is ON HOLD	+	The entry 7r47 is ON HOLD until Paper Publication

-	Authors: ~~Bridges, H.R., Blaza, J.N., Yin, Z., Chung, I., Hirst, J.~~	+	Authors:

-	Description: ~~Bovine complex I in the presence of IM1761092, deactive class iii (Composite map)~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Blaza, J.N]]
-	[[Category: Chung, I]]
-	[[Category: Hirst, J]]
-	[[Category: Bridges, H.R]]
-	[[Category: Yin, Z]]

OCA: Protected "7r47" [edit=sysop:move=sysop]

OCA — Wed, 16 Feb 2022 09:02:45 GMT

Protected "7r47" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:02, 16 February 2022

OCA: New page: '''Unreleased structure''' The entry 7r47 is ON HOLD Authors: Bridges, H.R., Blaza, J.N., Yin, Z., Chung, I., Hirst, J. Description: Bovine complex I in the presence of IM1761092, deac...

OCA — Wed, 16 Feb 2022 09:02:44 GMT

New page: '''Unreleased structure''' The entry 7r47 is ON HOLD Authors: Bridges, H.R., Blaza, J.N., Yin, Z., Chung, I., Hirst, J. Description: Bovine complex I in the presence of IM1761092, deac...

New page

'''Unreleased structure'''

The entry 7r47 is ON HOLD

Authors: Bridges, H.R., Blaza, J.N., Yin, Z., Chung, I., Hirst, J.

Description: Bovine complex I in the presence of IM1761092, deactive class iii (Composite map)
[[Category: Unreleased Structures]]
[[Category: Blaza, J.N]]
[[Category: Chung, I]]
[[Category: Hirst, J]]
[[Category: Bridges, H.R]]
[[Category: Yin, Z]]

7r47 - Revision history

OCA at 06:40, 8 February 2023

OCA at 04:58, 16 March 2022

OCA: Protected "7r47" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7r47 is ON HOLD Authors: Bridges, H.R., Blaza, J.N., Yin, Z., Chung, I., Hirst, J. Description: Bovine complex I in the presence of IM1761092, deac...