7rfb - Revision history

OCA at 16:30, 18 October 2023

OCA — Wed, 18 Oct 2023 16:30:34 GMT

←Older revision		Revision as of 16:30, 18 October 2023
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-	====	+	==Crystal structure of broadly neutralizing antibody mAb1198 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain==
-	<StructureSection load='7rfb' size='340' side='right'caption='[[7rfb]]' scene=''>	+	<StructureSection load='7rfb' size='340' side='right'caption='[[7rfb]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7rfb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepacivirus_C Hepacivirus C] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RFB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RFB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rfb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rfb OCA], [https://pdbe.org/7rfb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rfb RCSB], [https://www.ebi.ac.uk/pdbsum/7rfb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rfb ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rfb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rfb OCA], [https://pdbe.org/7rfb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rfb RCSB], [https://www.ebi.ac.uk/pdbsum/7rfb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rfb ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A2P0NE26_9HEPC A0A2P0NE26_9HEPC]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The high genetic diversity of hepatitis C virus (HCV) complicates effective vaccine development. We screened a cohort of 435 HCV-infected individuals and found that 2%-5% demonstrated outstanding HCV-neutralizing activity. From four of these patients, we isolated 310 HCV antibodies, including neutralizing antibodies with exceptional breadth and potency. High neutralizing activity was enabled by the use of the VH1-69 heavy-chain gene segment, somatic mutations within CDRH1, and CDRH2 hydrophobicity. Structural and mutational analyses revealed an important role for mutations replacing the serines at positions 30 and 31, as well as the presence of neutral and hydrophobic residues at the tip of the CDRH3. Based on these characteristics, we computationally created a de novo antibody with a fully synthetic VH1-69 heavy chain that efficiently neutralized multiple HCV genotypes. Our findings provide a deep understanding of the generation of broadly HCV-neutralizing antibodies that can guide the design of effective vaccine candidates.
		+
		+	Analysis of antibodies from HCV elite neutralizers identifies genetic determinants of broad neutralization.,Weber T, Potthoff J, Bizu S, Labuhn M, Dold L, Schoofs T, Horning M, Ercanoglu MS, Kreer C, Gieselmann L, Vanshylla K, Langhans B, Janicki H, Stroh LJ, Knops E, Nierhoff D, Spengler U, Kaiser R, Bjorkman PJ, Krey T, Bankwitz D, Pfeifer N, Pietschmann T, Flyak AI, Klein F Immunity. 2022 Feb 8;55(2):341-354.e7. doi: 10.1016/j.immuni.2021.12.003. Epub , 2022 Jan 5. PMID:34990590<ref>PMID:34990590</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7rfb" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hepacivirus C]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Bjorkman PJ]]
		+	[[Category: Flyak AI]]

OCA at 09:58, 12 January 2022

OCA — Wed, 12 Jan 2022 09:58:46 GMT

←Older revision		Revision as of 09:58, 12 January 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7rfb ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7rfb' size='340' side='right'caption='[[7rfb]]' scene=''>
-	~~Authors~~: ~~Flyak, A~~.I.~~, Bjorkman, P~~.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal~~ structure ~~of broadly neutralizing antibody mAb1198 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rfb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rfb OCA], [https://pdbe.org/7rfb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rfb RCSB], [https://www.ebi.ac.uk/pdbsum/7rfb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rfb ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Bjorkman~~, P.J]]	+	__TOC__
-	[[Category: ~~Flyak, A.I~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 05:06, 18 August 2021

OCA — Wed, 18 Aug 2021 05:06:17 GMT

←Older revision		Revision as of 05:06, 18 August 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7rfb is ON HOLD	+	The entry 7rfb is ON HOLD until Paper Publication

	Authors: Flyak, A.I., Bjorkman, P.J.		Authors: Flyak, A.I., Bjorkman, P.J.

OCA: Protected "7rfb" [edit=sysop:move=sysop]

OCA — Wed, 21 Jul 2021 08:03:09 GMT

Protected "7rfb" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:03, 21 July 2021

OCA: New page: '''Unreleased structure''' The entry 7rfb is ON HOLD Authors: Flyak, A.I., Bjorkman, P.J. Description: Crystal structure of broadly neutralizing antibody mAb1198 in complex with Hepati...

OCA — Wed, 21 Jul 2021 08:03:08 GMT

New page: '''Unreleased structure''' The entry 7rfb is ON HOLD Authors: Flyak, A.I., Bjorkman, P.J. Description: Crystal structure of broadly neutralizing antibody mAb1198 in complex with Hepati...

New page

'''Unreleased structure'''

The entry 7rfb is ON HOLD

Authors: Flyak, A.I., Bjorkman, P.J.

Description: Crystal structure of broadly neutralizing antibody mAb1198 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain
[[Category: Unreleased Structures]]
[[Category: Bjorkman, P.J]]
[[Category: Flyak, A.I]]