7rg4 - Revision history

OCA at 09:27, 25 October 2023

OCA — Wed, 25 Oct 2023 09:27:11 GMT

←Older revision		Revision as of 09:27, 25 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7rg4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RG4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7rg4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RG4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rg4 OCA], [https://pdbe.org/7rg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rg4 RCSB], [https://www.ebi.ac.uk/pdbsum/7rg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rg4 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rg4 OCA], [https://pdbe.org/7rg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rg4 RCSB], [https://www.ebi.ac.uk/pdbsum/7rg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rg4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 07:42, 8 March 2023

OCA — Wed, 08 Mar 2023 07:42:52 GMT

←Older revision		Revision as of 07:42, 8 March 2023
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-	====	+	==Importin alpha2 in complex with p50 NLS==
-	<StructureSection load='7rg4' size='340' side='right'caption='[[7rg4]]' scene=''>	+	<StructureSection load='7rg4' size='340' side='right'caption='[[7rg4]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7rg4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RG4 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rg4 OCA], [https://pdbe.org/7rg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rg4 RCSB], [https://www.ebi.ac.uk/pdbsum/7rg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rg4 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rg4 OCA], [https://pdbe.org/7rg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rg4 RCSB], [https://www.ebi.ac.uk/pdbsum/7rg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rg4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IMA1_MOUSE IMA1_MOUSE] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The MERS coronavirus (MERS-CoV) is a highly pathogenic, emerging virus that produces accessory proteins to antagonize the host innate immune response. The MERS-CoV ORF4b protein has been shown to bind preferentially to the nuclear import adapter IMPalpha3 in infected cells, thereby inhibiting NF-kappaB-dependent innate immune responses. Here, we report high-resolution structures of ORF4b bound to two distinct IMPalpha family members. Each exhibit highly similar binding mechanisms that, in both cases, lack a prototypical Lys bound at their P2 site. Mutations within the NLS region dramatically alter the mechanism of binding, which reverts to the canonical P2 Lys binding mechanism. Mutational studies confirm that the novel binding mechanism is important for its nuclear import, IMPalpha interaction, and inhibition of innate immune signaling pathways. In parallel, we determined structures of the nuclear binding domain of NF-kappaB component p50 bound to both IMPalpha2 and alpha3, demonstrating that p50 overlaps with the ORF4b binding sites, suggesting a basis for inhibition. Our results provide a detailed structural basis that explains how a virus can target the IMPalpha nuclear import adapter to impair immunity, and illustrate how small mutations in ORF4b, like those found in closely related coronaviruses such as HKU5, change the IMPalpha binding mechanism.
		+
		+	MERS-CoV ORF4b employs an unusual binding mechanism to target IMPalpha and block innate immunity.,Munasinghe TS, Edwards MR, Tsimbalyuk S, Vogel OA, Smith KM, Stewart M, Foster JK, Bosence LA, Aragao D, Roby JA, Basler CF, Forwood JK Nat Commun. 2022 Mar 25;13(1):1604. doi: 10.1038/s41467-022-28851-2. PMID:35338144<ref>PMID:35338144</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7rg4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Importin 3D structures\|Importin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Mus musculus]]
		+	[[Category: Aragao D]]
		+	[[Category: Forwood JK]]
		+	[[Category: Smith KM]]
		+	[[Category: Tsimbalyuk S]]

OCA at 06:21, 25 August 2021

OCA — Wed, 25 Aug 2021 06:21:09 GMT

←Older revision		Revision as of 06:21, 25 August 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7rg4 ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7rg4' size='340' side='right'caption='[[7rg4]]' scene=''>
-	~~Authors~~: ~~Smith, K~~.M.~~, Tsimbalyuk, S~~.~~, Aragao, D~~.~~, Forwood, J~~.K.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Importin alpha2 in complex with p50 NLS~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rg4 OCA], [https://pdbe.org/7rg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rg4 RCSB], [https://www.ebi.ac.uk/pdbsum/7rg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rg4 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Tsimbalyuk~~, S]]	+	__TOC__
-	[~~[Category~~: ~~Aragao, D~~]]	+	</StructureSection>
-	[[Category: ~~Forwood, J.K~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Smith, K.M~~]]	+	[[Category: Z-disk]]

OCA: Protected "7rg4" [edit=sysop:move=sysop]

OCA — Wed, 21 Jul 2021 08:04:18 GMT

Protected "7rg4" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:04, 21 July 2021

OCA: New page: '''Unreleased structure''' The entry 7rg4 is ON HOLD Authors: Smith, K.M., Tsimbalyuk, S., Aragao, D., Forwood, J.K. Description: Importin alpha2 in complex with p50 NLS [[Category: Un...

OCA — Wed, 21 Jul 2021 08:04:17 GMT

New page: '''Unreleased structure''' The entry 7rg4 is ON HOLD Authors: Smith, K.M., Tsimbalyuk, S., Aragao, D., Forwood, J.K. Description: Importin alpha2 in complex with p50 NLS [[Category: Un...

New page

'''Unreleased structure'''

The entry 7rg4 is ON HOLD

Authors: Smith, K.M., Tsimbalyuk, S., Aragao, D., Forwood, J.K.

Description: Importin alpha2 in complex with p50 NLS
[[Category: Unreleased Structures]]
[[Category: Tsimbalyuk, S]]
[[Category: Aragao, D]]
[[Category: Forwood, J.K]]
[[Category: Smith, K.M]]