OCA at 06:48, 10 May 2023

OCA — Wed, 10 May 2023 06:48:15 GMT

←Older revision		Revision as of 06:48, 10 May 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7rig is ~~ON HOLD until Paper~~ Publication	+	==Structure of ACLY-D1026A-substrates-warm==
		+	<StructureSection load='7rig' size='340' side='right'caption='[[7rig]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7rig]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RIG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=Q5B:(3S)-citryl-Coenzyme+A'>Q5B</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rig OCA], [https://pdbe.org/7rig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rig RCSB], [https://www.ebi.ac.uk/pdbsum/7rig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rig ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ACLY_HUMAN ACLY_HUMAN] ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.<ref>PMID:23932781</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ATP citrate lyase (ACLY) is the predominant nucleocytosolic source of acetyl-CoA and is aberrantly regulated in many diseases making it an attractive therapeutic target. Structural studies of ACLY reveal a central homotetrameric core citrate synthase homology (CSH) module flanked by acyl-CoA synthetase homology (ASH) domains, with ATP and citrate binding the ASH domain and CoA binding the ASH-CSH interface to produce acetyl-CoA and oxaloacetate products. The specific catalytic role of the CSH module and an essential D1026A residue contained within it has been a matter of debate. Here, we report biochemical and structural analysis of an ACLY-D1026A mutant demonstrating that this mutant traps a (3S)-citryl-CoA intermediate in the ASH domain in a configuration that is incompatible with the formation of acetyl-CoA, is able to convert acetyl-CoA and OAA to (3S)-citryl-CoA in the ASH domain, and can load CoA and unload acetyl-CoA in the CSH module. Together, this data support an allosteric role for the CSH module in ACLY catalysis.

-	~~Authors: Xuepeng, W~~., ~~Ronen~~, M.	+	Allosteric role of the citrate synthase homology domain of ATP citrate lyase.,Wei X, Schultz K, Pepper HL, Megill E, Vogt A, Snyder NW, Marmorstein R Nat Commun. 2023 Apr 19;14(1):2247. doi: 10.1038/s41467-023-37986-9. PMID:37076498<ref>PMID:37076498</ref>

-	~~Description: Structure~~ of ~~ACLY~~-~~D1026A~~-~~substrates-warm~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Xuepeng, W~~]]	+	<div class="pdbe-citations 7rig" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Ronen, M~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Marmorstein R]]
		+	[[Category: Wei X]]

OCA at 14:00, 22 December 2021

OCA — Wed, 22 Dec 2021 14:00:11 GMT

←Older revision		Revision as of 14:00, 22 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7rig is ON HOLD	+	The entry 7rig is ON HOLD until Paper Publication

	Authors: Xuepeng, W., Ronen, M.		Authors: Xuepeng, W., Ronen, M.

OCA: Protected "7rig" [edit=sysop:move=sysop]

OCA — Wed, 28 Jul 2021 09:37:12 GMT

Protected "7rig" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:37, 28 July 2021

OCA: New page: '''Unreleased structure''' The entry 7rig is ON HOLD Authors: Xuepeng, W., Ronen, M. Description: Structure of ACLY-D1026A-substrates-warm Category: Unreleased Structures [[Categor...

OCA — Wed, 28 Jul 2021 09:37:11 GMT

New page: '''Unreleased structure''' The entry 7rig is ON HOLD Authors: Xuepeng, W., Ronen, M. Description: Structure of ACLY-D1026A-substrates-warm Category: Unreleased Structures [[Categor...

New page

'''Unreleased structure'''

The entry 7rig is ON HOLD

Authors: Xuepeng, W., Ronen, M.

Description: Structure of ACLY-D1026A-substrates-warm
[[Category: Unreleased Structures]]
[[Category: Xuepeng, W]]
[[Category: Ronen, M]]

7rig - Revision history

OCA at 06:48, 10 May 2023

OCA at 14:00, 22 December 2021

OCA: Protected "7rig" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7rig is ON HOLD Authors: Xuepeng, W., Ronen, M. Description: Structure of ACLY-D1026A-substrates-warm Category: Unreleased Structures [[Categor...