OCA at 10:37, 6 April 2022

OCA — Wed, 06 Apr 2022 10:37:23 GMT

←Older revision		Revision as of 10:37, 6 April 2022
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	==Cryo-EM structure of monomeric TTYH2==		==Cryo-EM structure of monomeric TTYH2==
-	<StructureSection load='7rtv' size='340' side='right'caption='[[7rtv]]' scene=''>	+	<StructureSection load='7rtv' size='340' side='right'caption='[[7rtv]], [[Resolution\|resolution]] 3.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RTV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RTV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7rtv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RTV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RTV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rtv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rtv OCA], [https://pdbe.org/7rtv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rtv RCSB], [https://www.ebi.ac.uk/pdbsum/7rtv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rtv ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rtv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rtv OCA], [https://pdbe.org/7rtv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rtv RCSB], [https://www.ebi.ac.uk/pdbsum/7rtv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rtv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/TTYH2_MOUSE TTYH2_MOUSE]] Probable large-conductance Ca(2+)-activated chloride channel. May play a role in Ca(2+) signal transduction. May be involved in cell proliferation and cell aggregation (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tweety homologs (TTYHs) comprise a conserved family of transmembrane proteins found in eukaryotes with three members (TTYH1-3) in vertebrates. They are widely expressed in mammals including at high levels in the nervous system and have been implicated in cancers and other diseases including epilepsy, chronic pain, and viral infections. TTYHs have been reported to form Ca(2+)- and cell volume-regulated anion channels structurally distinct from any characterized protein family with potential roles in cell adhesion, migration, and developmental signaling. To provide insight into TTYH family structure and function, we determined cryo-EM structures of Mus musculus TTYH2 and TTYH3 in lipid nanodiscs. TTYH2 and TTYH3 adopt a previously unobserved fold which includes an extended extracellular domain with a partially solvent exposed pocket that may be an interaction site for hydrophobic molecules. In the presence of Ca(2+), TTYH2 and TTYH3 form homomeric cis-dimers bridged by extracellularly coordinated Ca(2+). Strikingly, in the absence of Ca(2+), TTYH2 forms trans-dimers that span opposing membranes across a ~130 A intermembrane space as well as a monomeric state. All TTYH structures lack ion conducting pathways and we do not observe TTYH2-dependent channel activity in cells. We conclude TTYHs are not pore forming subunits of anion channels and their function may involve Ca(2+)-dependent changes in quaternary structure, interactions with hydrophobic molecules near the extracellular membrane surface, and/or association with additional protein partners.
		+
		+	Structures of tweety homolog proteins TTYH2 and TTYH3 reveal a Ca(2+)-dependent switch from intra- to intermembrane dimerization.,Li B, Hoel CM, Brohawn SG Nat Commun. 2021 Nov 25;12(1):6913. doi: 10.1038/s41467-021-27283-8. PMID:34824283<ref>PMID:34824283</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7rtv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brohawn SG]]	+	[[Category: Brohawn, S G]]
-	[[Category: Hoel CM]]	+	[[Category: Hoel, C M]]
-	[[Category: Li B]]	+	[[Category: Li, B]]
		+	[[Category: Membrane protein]]
		+	[[Category: Monomer]]
		+	[[Category: Ttyh2]]

OCA at 06:53, 22 September 2021

OCA — Wed, 22 Sep 2021 06:53:41 GMT

←Older revision		Revision as of 06:53, 22 September 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7rtv ~~is ON HOLD~~	+	==Cryo-EM structure of monomeric TTYH2==
-		+	<StructureSection load='7rtv' size='340' side='right'caption='[[7rtv]]' scene=''>
-	~~Authors~~: Li, B., ~~Brohawn~~, S.G., ~~Hoel~~, C.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RTV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RTV FirstGlance]. <br>
-	~~Description: Cryo-EM structure of monomeric TTYH2~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rtv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rtv OCA], [https://pdbe.org/7rtv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rtv RCSB], [https://www.ebi.ac.uk/pdbsum/7rtv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rtv ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Hoel, C.M~~]]	+	__TOC__
-	[[Category: ~~Brohawn, S.G~~]]	+	</StructureSection>
-	[[Category: Li, B]]	+	[[Category: Large Structures]]
		+	[[Category: Brohawn SG]]
		+	[[Category: Hoel CM]]
		+	[[Category: Li B]]

OCA: Protected "7rtv" [edit=sysop:move=sysop]

OCA — Wed, 25 Aug 2021 05:17:54 GMT

Protected "7rtv" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:17, 25 August 2021

OCA: New page: '''Unreleased structure''' The entry 7rtv is ON HOLD Authors: Li, B., Brohawn, S.G., Hoel, C.M. Description: Cryo-EM structure of monomeric TTYH2 Category: Unreleased Structures [[...

OCA — Wed, 25 Aug 2021 05:17:53 GMT

New page: '''Unreleased structure''' The entry 7rtv is ON HOLD Authors: Li, B., Brohawn, S.G., Hoel, C.M. Description: Cryo-EM structure of monomeric TTYH2 Category: Unreleased Structures [[...

New page

'''Unreleased structure'''

The entry 7rtv is ON HOLD

Authors: Li, B., Brohawn, S.G., Hoel, C.M.

Description: Cryo-EM structure of monomeric TTYH2
[[Category: Unreleased Structures]]
[[Category: Hoel, C.M]]
[[Category: Brohawn, S.G]]
[[Category: Li, B]]

7rtv - Revision history

OCA at 10:37, 6 April 2022

OCA at 06:53, 22 September 2021

OCA: Protected "7rtv" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7rtv is ON HOLD Authors: Li, B., Brohawn, S.G., Hoel, C.M. Description: Cryo-EM structure of monomeric TTYH2 Category: Unreleased Structures [[...