OCA at 08:15, 25 May 2022

OCA — Wed, 25 May 2022 08:15:30 GMT

←Older revision		Revision as of 08:15, 25 May 2022
Line 1:		Line 1:

	==afTMEM16 lipid scramblase in C18 lipid nanodiscs in the absence of Ca2+==		==afTMEM16 lipid scramblase in C18 lipid nanodiscs in the absence of Ca2+==
-	<StructureSection load='7rxb' size='340' side='right'caption='[[7rxb]]' scene=''>	+	<StructureSection load='7rxb' size='340' side='right'caption='[[7rxb]], [[Resolution\|resolution]] 3.07Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RXB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7rxb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RXB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rxb OCA], [https://pdbe.org/7rxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rxb RCSB], [https://www.ebi.ac.uk/pdbsum/7rxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rxb ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGW:(1R)-2-{[(S)-{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(HEXADECANOYLOXY)METHYL]ETHYL+(9Z)-OCTADEC-9-ENOATE'>PGW</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7rwj\|7rwj]]</div></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rxb OCA], [https://pdbe.org/7rxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rxb RCSB], [https://www.ebi.ac.uk/pdbsum/7rxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rxb ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	TMEM16 scramblases dissipate the plasma membrane lipid asymmetry to activate multiple eukaryotic cellular pathways. Scrambling was proposed to occur with lipid headgroups moving between leaflets through a membrane-spanning hydrophilic groove. Direct information on lipid-groove interactions is lacking. We report the 2.3 A resolution cryogenic electron microscopy structure of the nanodisc-reconstituted Ca(2+)-bound afTMEM16 scramblase showing how rearrangement of individual lipids at the open pathway results in pronounced membrane thinning. Only the groove's intracellular vestibule contacts lipids, and mutagenesis suggests scrambling does not require specific protein-lipid interactions with the extracellular vestibule. We find scrambling can occur outside a closed groove in thinner membranes and is inhibited in thicker membranes, despite an open pathway. Our results show afTMEM16 thins the membrane to enable scrambling and that an open hydrophilic pathway is not a structural requirement to allow rapid transbilayer movement of lipids. This mechanism could be extended to other scramblases lacking a hydrophilic groove.
		+
		+	TMEM16 scramblases thin the membrane to enable lipid scrambling.,Falzone ME, Feng Z, Alvarenga OE, Pan Y, Lee B, Cheng X, Fortea E, Scheuring S, Accardi A Nat Commun. 2022 May 11;13(1):2604. doi: 10.1038/s41467-022-30300-z. PMID:35562175<ref>PMID:35562175</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7rxb" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Accardi A]]	+	[[Category: Accardi, A]]
-	[[Category: Feng Z]]	+	[[Category: Feng, Z]]
		+	[[Category: Lipid scrambling]]
		+	[[Category: Lipid transport]]
		+	[[Category: Tmem16]]

OCA at 10:16, 18 May 2022

OCA — Wed, 18 May 2022 10:16:29 GMT

←Older revision		Revision as of 10:16, 18 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7rxb ~~is ON HOLD until Paper Publication~~	+	==afTMEM16 lipid scramblase in C18 lipid nanodiscs in the absence of Ca2+==
-		+	<StructureSection load='7rxb' size='340' side='right'caption='[[7rxb]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RXB FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rxb OCA], [https://pdbe.org/7rxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rxb RCSB], [https://www.ebi.ac.uk/pdbsum/7rxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rxb ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Accardi A]]
		+	[[Category: Feng Z]]

OCA: Protected "7rxb" [edit=sysop:move=sysop]

OCA — Wed, 08 Sep 2021 07:46:47 GMT

Protected "7rxb" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:46, 8 September 2021

OCA: New page: '''Unreleased structure''' The entry 7rxb is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 08 Sep 2021 07:46:46 GMT

New page: '''Unreleased structure''' The entry 7rxb is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7rxb is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

7rxb - Revision history

OCA at 08:15, 25 May 2022

OCA at 10:16, 18 May 2022

OCA: Protected "7rxb" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7rxb is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures