7s17 - Revision history

OCA at 09:31, 25 October 2023

OCA — Wed, 25 Oct 2023 09:31:51 GMT

←Older revision		Revision as of 09:31, 25 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7s17]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S17 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7s17]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S17 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s17 OCA], [https://pdbe.org/7s17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s17 RCSB], [https://www.ebi.ac.uk/pdbsum/7s17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s17 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.36Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s17 OCA], [https://pdbe.org/7s17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s17 RCSB], [https://www.ebi.ac.uk/pdbsum/7s17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s17 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 07:46, 11 January 2023

OCA — Wed, 11 Jan 2023 07:46:23 GMT

←Older revision		Revision as of 07:46, 11 January 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7s17 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of human G3BP1-NTF2 with three mutations- F15W, F33W, and F124W==
		+	<StructureSection load='7s17' size='340' side='right'caption='[[7s17]], [[Resolution\|resolution]] 2.36Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7s17]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S17 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s17 OCA], [https://pdbe.org/7s17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s17 RCSB], [https://www.ebi.ac.uk/pdbsum/7s17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s17 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G3BP1_HUMAN G3BP1_HUMAN] May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.<ref>PMID:9889278</ref> <ref>PMID:11604510</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Intrinsically disordered proteins (IDPs) often coordinate transient interactions with multiple proteins to mediate complex signals within large protein networks. Among these, the IDP hub protein G3BP1 can form complexes with cytoplasmic phosphoprotein Caprin1 and ubiquitin peptidase USP10; the resulting control of USP10 activity contributes to a pathogenic virulence system that targets endocytic recycling of the ion channel CFTR. However, while the identities of protein interactors are known for many IDP hub proteins, the relationship between pairwise affinities and the extent of protein recruitment and activity is not well understood. Here, we describe in vitro analysis of these G3BP1 affinities and show tryptophan substitutions of specific G3BP1 residues reduce its affinity for both USP10 and Caprin1. We show that these same mutations reduce the stability of complexes between the full-length proteins, suggesting that copurification can serve as a surrogate measure of interaction strength. The crystal structure of G3BP1 TripleW (F15W/F33W/F124W) mutant reveals a clear reorientation of the side chain of W33, creating a steric clash with USP10 and Caprin1. Furthermore, an amino-acid scan of USP10 and Caprin1 peptides reveals similarities and differences in the ability to substitute residues in the core motifs as well as specific substitutions with the potential to create higher affinity peptides. Taken together, these data show that small changes in component binding affinities can have significant effects on the composition of cellular interaction hubs. These specific protein mutations can be harnessed to manipulate complex protein networks, informing future investigations into roles of these networks in cellular processes.

-	~~Authors:~~ Sheehan, ~~C.T.~~, Madden~~, D~~.R.	+	Tryptophan mutations in G3BP1 tune the stability of a cellular signaling hub by weakening transient interactions with Caprin1 and USP10.,Sheehan CT, Hampton TH, Madden DR J Biol Chem. 2022 Dec;298(12):102552. doi: 10.1016/j.jbc.2022.102552. Epub 2022 , Sep 29. PMID:36183834<ref>PMID:36183834</ref>

-	~~Description: Crystal structure~~ of ~~human G3BP1~~-~~NTF2 with three mutations~~-~~F15W, F33W, and F124W~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Sheehan, C.T~~]]	+	<div class="pdbe-citations 7s17" style="background-color:#fffaf0;"></div>
-	[[Category: Madden~~, D.R~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Madden DR]]
		+	[[Category: Sheehan CT]]

OCA at 10:21, 13 October 2021

OCA — Wed, 13 Oct 2021 10:21:40 GMT

←Older revision		Revision as of 10:21, 13 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7s17 is ON HOLD	+	The entry 7s17 is ON HOLD until Paper Publication

	Authors: Sheehan, C.T., Madden, D.R.		Authors: Sheehan, C.T., Madden, D.R.

OCA: Protected "7s17" [edit=sysop:move=sysop]

OCA — Wed, 08 Sep 2021 07:52:52 GMT

Protected "7s17" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:52, 8 September 2021

OCA: New page: '''Unreleased structure''' The entry 7s17 is ON HOLD Authors: Sheehan, C.T., Madden, D.R. Description: Crystal structure of human G3BP1-NTF2 with three mutations-F15W, F33W, and F124W ...

OCA — Wed, 08 Sep 2021 07:52:51 GMT

New page: '''Unreleased structure''' The entry 7s17 is ON HOLD Authors: Sheehan, C.T., Madden, D.R. Description: Crystal structure of human G3BP1-NTF2 with three mutations-F15W, F33W, and F124W ...

New page

'''Unreleased structure'''

The entry 7s17 is ON HOLD

Authors: Sheehan, C.T., Madden, D.R.

Description: Crystal structure of human G3BP1-NTF2 with three mutations-F15W, F33W, and F124W
[[Category: Unreleased Structures]]
[[Category: Sheehan, C.T]]
[[Category: Madden, D.R]]