7s6b - Revision history

OCA at 10:42, 22 May 2024

OCA — Wed, 22 May 2024 10:42:18 GMT

←Older revision		Revision as of 10:42, 22 May 2024
Line 1:		Line 1:

-	====	+	==Crystal structure of modular polyketide synthase apo-Lsd14 from the Lasalocid biosynthesis pathway, trapped in the transacylation step==
-	<StructureSection load='7s6b' size='340' side='right'caption='[[7s6b]]' scene=''>	+	<StructureSection load='7s6b' size='340' side='right'caption='[[7s6b]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7s6b]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lasalocidi Streptomyces lasalocidi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S6B FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s6b OCA], [https://pdbe.org/7s6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s6b RCSB], [https://www.ebi.ac.uk/pdbsum/7s6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s6b ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s6b OCA], [https://pdbe.org/7s6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s6b RCSB], [https://www.ebi.ac.uk/pdbsum/7s6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s6b ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B6ZK67_STRLS B6ZK67_STRLS]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type I modular polyketide synthases are homodimeric multidomain assembly line enzymes that synthesize a variety of polyketide natural products by performing polyketide chain extension and beta-keto group modification reactions. We determined the 2.4-angstrom-resolution x-ray crystal structure and the 3.1-angstrom-resolution cryo-electron microscopy structure of the Lsd14 polyketide synthase, stalled at the transacylation and condensation steps, respectively. These structures revealed how the constituent domains are positioned relative to each other, how they rearrange depending on the step in the reaction cycle, and the specific interactions formed between the domains. Like the evolutionarily related mammalian fatty acid synthase, Lsd14 contains two reaction chambers, but only one chamber in Lsd14 has the full complement of catalytic domains, indicating that only one chamber produces the polyketide product at any given time.
		+
		+	Modular polyketide synthase contains two reaction chambers that operate asynchronously.,Bagde SR, Mathews II, Fromme JC, Kim CY Science. 2021 Nov 5;374(6568):723-729. doi: 10.1126/science.abi8532. Epub 2021 , Nov 4. PMID:34735234<ref>PMID:34735234</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7s6b" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Streptomyces lasalocidi]]
		+	[[Category: Bagde SR]]
		+	[[Category: Kim C-Y]]
		+	[[Category: Mathews II]]

OCA at 14:33, 3 November 2021

OCA — Wed, 03 Nov 2021 14:33:04 GMT

←Older revision		Revision as of 14:33, 3 November 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7s6b ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7s6b' size='340' side='right'caption='[[7s6b]]' scene=''>
-	~~Authors~~: ~~Bagde, S~~.R.~~, Mathews, I~~.I.~~, Kim, C~~.-Y.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of modular polyketide synthase apo~~-~~Lsd14 from the Lasalocid biosynthesis pathway~~, ~~trapped in the transacylation step~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s6b OCA], [https://pdbe.org/7s6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s6b RCSB], [https://www.ebi.ac.uk/pdbsum/7s6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s6b ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Bagde~~, S.R]]	+	__TOC__
-	[[Category: ~~Mathews, I.I~~]]	+	</StructureSection>
-	[[Category: ~~Kim, C.~~-Y]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7s6b" [edit=sysop:move=sysop]

OCA — Wed, 22 Sep 2021 05:39:31 GMT

Protected "7s6b" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:39, 22 September 2021

OCA: New page: '''Unreleased structure''' The entry 7s6b is ON HOLD Authors: Bagde, S.R., Mathews, I.I., Kim, C.-Y. Description: Crystal structure of modular polyketide synthase apo-Lsd14 from the La...

OCA — Wed, 22 Sep 2021 05:39:29 GMT

New page: '''Unreleased structure''' The entry 7s6b is ON HOLD Authors: Bagde, S.R., Mathews, I.I., Kim, C.-Y. Description: Crystal structure of modular polyketide synthase apo-Lsd14 from the La...

New page

'''Unreleased structure'''

The entry 7s6b is ON HOLD

Authors: Bagde, S.R., Mathews, I.I., Kim, C.-Y.

Description: Crystal structure of modular polyketide synthase apo-Lsd14 from the Lasalocid biosynthesis pathway, trapped in the transacylation step
[[Category: Unreleased Structures]]
[[Category: Bagde, S.R]]
[[Category: Mathews, I.I]]
[[Category: Kim, C.-Y]]