OCA at 16:45, 18 October 2023

OCA — Wed, 18 Oct 2023 16:45:40 GMT

←Older revision		Revision as of 16:45, 18 October 2023
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-	====	+	==Complex structure of Methane monooxygenase hydroxylase and regulatory subunit DBL2==
-	<StructureSection load='7s6q' size='340' side='right'caption='[[7s6q]]' scene=''>	+	<StructureSection load='7s6q' size='340' side='right'caption='[[7s6q]], [[Resolution\|resolution]] 1.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7s6q]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylosinus_trichosporium_OB3b Methylosinus trichosporium OB3b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S6Q FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s6q OCA], [https://pdbe.org/7s6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s6q RCSB], [https://www.ebi.ac.uk/pdbsum/7s6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s6q ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.96Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s6q OCA], [https://pdbe.org/7s6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s6q RCSB], [https://www.ebi.ac.uk/pdbsum/7s6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s6q ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MEMA_METTR MEMA_METTR] Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Full activity of soluble methane monooxygenase (sMMO) depends upon the formation of a 1:1 complex of the regulatory protein MMOB with each alpha subunit of the (alphabetagamma)(2) hydroxylase, sMMOH. Previous studies have shown that mutations in the core region of MMOB and in the N- and C-termini cause dramatic changes in the rate constants for steps in the sMMOH reaction cycle. Here, X-ray crystal structures are reported for the sMMOH complex with two double variants within the core region of MMOB, DBL1 (N107G/S110A), and DBL2 (S109A/T111A), as well as two variants in the MMOB N-terminal region, H33A and H5A. DBL1 causes a 150-fold decrease in the formation rate constant of the reaction cycle intermediate P, whereas DBL2 accelerates the reaction of the dinuclear Fe(IV) intermediate Q with substrates larger than methane by three- to fourfold. H33A also greatly slows P formation, while H5A modestly slows both formation of Q and its reactions with substrates. Complexation with DBL1 or H33A alters the position of sMMOH residue R245, which is part of a conserved hydrogen-bonding network encompassing the active site diiron cluster where P is formed. Accordingly, electron paramagnetic resonance spectra of sMMOH:DBL1 and sMMOH:H33A complexes differ markedly from that of sMMOH:MMOB, showing an altered electronic environment. In the sMMOH:DBL2 complex, the position of M247 in sMMOH is altered such that it enlarges a molecular tunnel associated with substrate entry into the active site. The H5A variant causes only subtle structural changes despite its kinetic effects, emphasizing the precise alignment of sMMOH and MMOB required for efficient catalysis.
		+
		+	X-ray Crystal Structures of Methane Monooxygenase Hydroxylase Complexes with Variants of Its Regulatory Component: Correlations with Altered Reaction Cycle Dynamics.,Jones JC, Banerjee R, Semonis MM, Shi K, Aihara H, Lipscomb JD Biochemistry. 2022 Jan 4;61(1):21-33. doi: 10.1021/acs.biochem.1c00673. Epub 2021 , Dec 15. PMID:34910460<ref>PMID:34910460</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7s6q" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Methane monooxygenase 3D structures\|Methane monooxygenase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Methylosinus trichosporium OB3b]]
		+	[[Category: Aihara H]]
		+	[[Category: Banerjee R]]
		+	[[Category: Johns JC]]
		+	[[Category: Lipscomb JD]]
		+	[[Category: Semonis MM]]
		+	[[Category: Shi K]]

OCA at 14:11, 29 December 2021

OCA — Wed, 29 Dec 2021 14:11:30 GMT

←Older revision		Revision as of 14:11, 29 December 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7s6q ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7s6q' size='340' side='right'caption='[[7s6q]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s6q OCA], [https://pdbe.org/7s6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s6q RCSB], [https://www.ebi.ac.uk/pdbsum/7s6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s6q ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 15:04, 20 October 2021

OCA — Wed, 20 Oct 2021 15:04:11 GMT

←Older revision		Revision as of 15:04, 20 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7s6q is ON HOLD	+	The entry 7s6q is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7s6q" [edit=sysop:move=sysop]

OCA — Wed, 22 Sep 2021 05:40:19 GMT

Protected "7s6q" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:40, 22 September 2021

OCA: New page: '''Unreleased structure''' The entry 7s6q is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Sep 2021 05:40:18 GMT

New page: '''Unreleased structure''' The entry 7s6q is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7s6q is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7s6q - Revision history

OCA at 16:45, 18 October 2023

OCA at 14:11, 29 December 2021

OCA at 15:04, 20 October 2021

OCA: Protected "7s6q" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7s6q is ON HOLD Authors: Description: Category: Unreleased Structures