7s7v - Revision history

OCA at 09:34, 25 October 2023

OCA — Wed, 25 Oct 2023 09:34:02 GMT

←Older revision		Revision as of 09:34, 25 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7s7v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacter_sp._X513 Thermoanaerobacter sp. X513]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S7V FirstGlance]. <br>		<table><tr><td colspan='2'>[[7s7v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacter_sp._X513 Thermoanaerobacter sp. X513]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S7V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s7v OCA], [https://pdbe.org/7s7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s7v RCSB], [https://www.ebi.ac.uk/pdbsum/7s7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s7v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s7v OCA], [https://pdbe.org/7s7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s7v RCSB], [https://www.ebi.ac.uk/pdbsum/7s7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s7v ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:56, 8 February 2023

OCA — Wed, 08 Feb 2023 06:56:57 GMT

←Older revision		Revision as of 06:56, 8 February 2023
Line 3:		Line 3:
	<StructureSection load='7s7v' size='340' side='right'caption='[[7s7v]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='7s7v' size='340' side='right'caption='[[7s7v]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7s7v]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S7V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7s7v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacter_sp._X513 Thermoanaerobacter sp. X513]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S7V FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s7v OCA], [https://pdbe.org/7s7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s7v RCSB], [https://www.ebi.ac.uk/pdbsum/7s7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s7v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s7v OCA], [https://pdbe.org/7s7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s7v RCSB], [https://www.ebi.ac.uk/pdbsum/7s7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s7v ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Nicotinic partial agonists provide an accepted aid for smoking cessation and thus contribute to decreasing tobacco-related disease. Improved drugs constitute a continued area of study. However, there remains no reductionist method to examine the cellular and subcellular pharmacokinetic properties of these compounds in living cells. Here, we developed new intensity-based drug-sensing fluorescent reporters (iDrugSnFRs) for the nicotinic partial agonists dianicline, cytisine, and two cytisine derivatives - 10-fluorocytisine and 9-bromo-10-ethylcytisine. We report the first atomic-scale structures of liganded periplasmic binding protein-based biosensors, accelerating development of iDrugSnFRs and also explaining the activation mechanism. The nicotinic iDrugSnFRs detect their drug partners in solution, as well as at the plasma membrane (PM) and in the endoplasmic reticulum (ER) of cell lines and mouse hippocampal neurons. At the PM, the speed of solution changes limits the growth and decay rates of the fluorescence response in almost all cases. In contrast, we found that rates of membrane crossing differ among these nicotinic drugs by >30-fold. The new nicotinic iDrugSnFRs provide insight into the real-time pharmacokinetic properties of nicotinic agonists and provide a methodology whereby iDrugSnFRs can inform both pharmaceutical neuroscience and addiction neuroscience.
-
-	Fluorescence activation mechanism and imaging of drug permeation with new sensors for smoking-cessation ligands.,Nichols AL, Blumenfeld Z, Fan C, Luebbert L, Blom AEM, Cohen BN, Marvin JS, Borden PM, Kim CH, Muthusamy AK, Shivange AV, Knox HJ, Campello HR, Wang JH, Dougherty DA, Looger LL, Gallagher T, Rees DC, Lester HA Elife. 2022 Jan 4;11. pii: 74648. doi: 10.7554/eLife.74648. PMID:34982029<ref>PMID:34982029</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 7s7v" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Fan, C~~]]	+	[[Category: Thermoanaerobacter sp. X513]]
-	[[Category: ~~Lester, H A]]~~	+	[[Category: Fan C]]
-	~~[[Category: Looger, L L]]~~	+	[[Category: Lester HA]]
-	~~[[Category: Rees, D~~ C]]	+	[[Category: Looger LL]]
-	[[Category: ~~Shivange, A V~~]]	+	[[Category: Rees DC]]
-	[[Category: ~~Choline-binding protein~~]]	+	[[Category: Shivange AV]]
-	[[Category: ~~Fluorescent protein~~]]	+
-	[[Category: ~~Fluorescent sensor]]~~	+
-	~~[[Category: Gfp]]~~	+
-	~~[[Category: Nicotine]]~~	+
-	~~[[Category: Nicotine-binding protein]]~~	+
-	~~[[Category: Periplasmic binding protein]]~~	+
-	~~[[Category: Pharmacokinetic sensor~~]]	+

OCA at 11:53, 27 April 2022

OCA — Wed, 27 Apr 2022 11:53:56 GMT

←Older revision		Revision as of 11:53, 27 April 2022
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s7v OCA], [https://pdbe.org/7s7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s7v RCSB], [https://www.ebi.ac.uk/pdbsum/7s7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s7v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s7v OCA], [https://pdbe.org/7s7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s7v RCSB], [https://www.ebi.ac.uk/pdbsum/7s7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s7v ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nicotinic partial agonists provide an accepted aid for smoking cessation and thus contribute to decreasing tobacco-related disease. Improved drugs constitute a continued area of study. However, there remains no reductionist method to examine the cellular and subcellular pharmacokinetic properties of these compounds in living cells. Here, we developed new intensity-based drug-sensing fluorescent reporters (iDrugSnFRs) for the nicotinic partial agonists dianicline, cytisine, and two cytisine derivatives - 10-fluorocytisine and 9-bromo-10-ethylcytisine. We report the first atomic-scale structures of liganded periplasmic binding protein-based biosensors, accelerating development of iDrugSnFRs and also explaining the activation mechanism. The nicotinic iDrugSnFRs detect their drug partners in solution, as well as at the plasma membrane (PM) and in the endoplasmic reticulum (ER) of cell lines and mouse hippocampal neurons. At the PM, the speed of solution changes limits the growth and decay rates of the fluorescence response in almost all cases. In contrast, we found that rates of membrane crossing differ among these nicotinic drugs by >30-fold. The new nicotinic iDrugSnFRs provide insight into the real-time pharmacokinetic properties of nicotinic agonists and provide a methodology whereby iDrugSnFRs can inform both pharmaceutical neuroscience and addiction neuroscience.
		+
		+	Fluorescence activation mechanism and imaging of drug permeation with new sensors for smoking-cessation ligands.,Nichols AL, Blumenfeld Z, Fan C, Luebbert L, Blom AEM, Cohen BN, Marvin JS, Borden PM, Kim CH, Muthusamy AK, Shivange AV, Knox HJ, Campello HR, Wang JH, Dougherty DA, Looger LL, Gallagher T, Rees DC, Lester HA Elife. 2022 Jan 4;11. pii: 74648. doi: 10.7554/eLife.74648. PMID:34982029<ref>PMID:34982029</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7s7v" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:03, 3 November 2021

OCA — Wed, 03 Nov 2021 16:03:45 GMT

←Older revision		Revision as of 16:03, 3 November 2021
Line 1:		Line 1:

-	==Crystal structure of ~~iNicSnFR 3.0~~ Fluorescent Nicotine Sensor==	+	==Crystal structure of iNicSnFR3a Fluorescent Nicotine Sensor==
-	<StructureSection load='7s7v' size='340' side='right'caption='[[7s7v]]' scene=''>	+	<StructureSection load='7s7v' size='340' side='right'caption='[[7s7v]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S7V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7s7v]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S7V FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s7v OCA], [https://pdbe.org/7s7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s7v RCSB], [https://www.ebi.ac.uk/pdbsum/7s7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s7v ProSAT]</span></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s7v OCA], [https://pdbe.org/7s7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s7v RCSB], [https://www.ebi.ac.uk/pdbsum/7s7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s7v ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fan C]]	+	[[Category: Fan, C]]
-	[[Category: Lester HA]]	+	[[Category: Lester, H A]]
-	[[Category: Looger LL]]	+	[[Category: Looger, L L]]
-	[[Category: Rees DC]]	+	[[Category: Rees, D C]]
-	[[Category: Shivange AV]]	+	[[Category: Shivange, A V]]
		+	[[Category: Choline-binding protein]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Fluorescent sensor]]
		+	[[Category: Gfp]]
		+	[[Category: Nicotine]]
		+	[[Category: Nicotine-binding protein]]
		+	[[Category: Periplasmic binding protein]]
		+	[[Category: Pharmacokinetic sensor]]

OCA at 11:47, 13 October 2021

OCA — Wed, 13 Oct 2021 11:47:31 GMT

←Older revision		Revision as of 11:47, 13 October 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7s7v ~~is ON HOLD~~	+	==Crystal structure of iNicSnFR 3.0 Fluorescent Nicotine Sensor==
-		+	<StructureSection load='7s7v' size='340' side='right'caption='[[7s7v]]' scene=''>
-	~~Authors~~: ~~Fan, C~~., ~~Shivange, A~~.V., ~~Looger~~, L.L., ~~Lester, H~~.A., ~~Rees, D~~.C.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S7V FirstGlance]. <br>
-	~~Description: Crystal structure of iNicSnFR 3.0 Fluorescent Nicotine Sensor~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s7v OCA], [https://pdbe.org/7s7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s7v RCSB], [https://www.ebi.ac.uk/pdbsum/7s7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s7v ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Shivange, A.V~~]]	+	__TOC__
-	[[Category: ~~Rees, D.C~~]]	+	</StructureSection>
-	[[Category: ~~Lester, H.A~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Looger, L.L~~]]	+	[[Category: Fan C]]
-	[[Category: ~~Fan, C~~]]	+	[[Category: Lester HA]]
		+	[[Category: Looger LL]]
		+	[[Category: Rees DC]]
		+	[[Category: Shivange AV]]

OCA: Protected "7s7v" [edit=sysop:move=sysop]

OCA — Wed, 29 Sep 2021 07:45:53 GMT

Protected "7s7v" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:45, 29 September 2021

OCA: New page: '''Unreleased structure''' The entry 7s7v is ON HOLD Authors: Fan, C., Shivange, A.V., Looger, L.L., Lester, H.A., Rees, D.C. Description: Crystal structure of iNicSnFR 3.0 Fluorescent...

OCA — Wed, 29 Sep 2021 07:45:52 GMT

New page: '''Unreleased structure''' The entry 7s7v is ON HOLD Authors: Fan, C., Shivange, A.V., Looger, L.L., Lester, H.A., Rees, D.C. Description: Crystal structure of iNicSnFR 3.0 Fluorescent...

New page

'''Unreleased structure'''

The entry 7s7v is ON HOLD

Authors: Fan, C., Shivange, A.V., Looger, L.L., Lester, H.A., Rees, D.C.

Description: Crystal structure of iNicSnFR 3.0 Fluorescent Nicotine Sensor
[[Category: Unreleased Structures]]
[[Category: Shivange, A.V]]
[[Category: Rees, D.C]]
[[Category: Lester, H.A]]
[[Category: Looger, L.L]]
[[Category: Fan, C]]