OCA at 17:02, 15 December 2021

OCA — Wed, 15 Dec 2021 17:02:02 GMT

←Older revision		Revision as of 17:02, 15 December 2021
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	==Cryo-EM Structure of dolphin Prestin: Inhibited I (Chloride + Salicylate)==		==Cryo-EM Structure of dolphin Prestin: Inhibited I (Chloride + Salicylate)==
-	<StructureSection load='7s9a' size='340' side='right'caption='[[7s9a]]' scene=''>	+	<StructureSection load='7s9a' size='340' side='right'caption='[[7s9a]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S9A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S9A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7s9a]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S9A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S9A FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s9a OCA], [https://pdbe.org/7s9a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s9a RCSB], [https://www.ebi.ac.uk/pdbsum/7s9a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s9a ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s9a OCA], [https://pdbe.org/7s9a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s9a RCSB], [https://www.ebi.ac.uk/pdbsum/7s9a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s9a ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/D7PC76_TURTR D7PC76_TURTR]] Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ.[RuleBase:RU362052]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The voltage-dependent motor protein Prestin (SLC26A5) is responsible for the electromotive behavior of outer hair cells and underlies the cochlear amplifier(1). Knock out or impairment of Prestin causes severe hearing loss(2-5). Despite Prestin's key role in hearing, the mechanism by which mammalian Prestin senses voltage and transduces it into cellular-scale movements (electromotility) is poorly understood. Here, we determined the structure of dolphin Prestin in six distinct states using single particle cryo-electron microscopy. Our structural and functional data suggest that Prestin adopts a unique and complex set of states, tunable by the identity of bound anions (Cl(-) or SO4(2-)). Salicylate, a drug that can cause reversible hearing loss, competes for the anion-binding site of Prestin, and inhibits its function by immobilizing Prestin in a novel conformation. Our data suggests that the bound anion together with its coordinating charged residues and helical dipole act as a dynamic voltage sensor. Analysis of all anion-dependent conformations reveals how structural rearrangements in the voltage sensor are coupled to conformational transitions at the protein-membrane interface, suggesting a novel mechanism of area expansion. Visualization of Prestin's electromotility cycle distinguishes Prestin from closely related SLC26 anion transporters, highlighting the basis for evolutionary specialization of the mammalian cochlear amplifier at high resolution.
		+
		+	Prestin's conformational cycle underlies outer hair cell electromotility.,Bavi N, Clark MD, Contreras GF, Shen R, Reddy BG, Milewski W, Perozo E Nature. 2021 Oct 25. pii: 10.1038/s41586-021-04152-4. doi:, 10.1038/s41586-021-04152-4. PMID:34695838<ref>PMID:34695838</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7s9a" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bavi N]]	+	[[Category: Bavi, N]]
-	[[Category: Clark MD]]	+	[[Category: Clark, M D]]
-	[[Category: Contreras GF]]	+	[[Category: Contreras, G F]]
-	[[Category: Milewski W]]	+	[[Category: Milewski, W]]
-	[[Category: Perozo E]]	+	[[Category: Perozo, E]]
-	[[Category: Reddy BG]]	+	[[Category: Reddy, B G]]
-	[[Category: Shen R]]	+	[[Category: Shen, R]]
		+	[[Category: Deafness]]
		+	[[Category: Echolocation]]
		+	[[Category: Electromotility]]
		+	[[Category: Frequency sensation]]
		+	[[Category: Hearing]]
		+	[[Category: Mechanotransduction]]
		+	[[Category: Motor protein]]
		+	[[Category: Outer hair cell]]
		+	[[Category: Slc26]]
		+	[[Category: Slc26a5]]

OCA at 14:33, 3 November 2021

OCA — Wed, 03 Nov 2021 14:33:10 GMT

←Older revision		Revision as of 14:33, 3 November 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7s9a is ~~ON HOLD until Paper Publication~~	+	==Cryo-EM Structure of dolphin Prestin: Inhibited I (Chloride + Salicylate)==
-		+	<StructureSection load='7s9a' size='340' side='right'caption='[[7s9a]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S9A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S9A FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s9a OCA], [https://pdbe.org/7s9a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s9a RCSB], [https://www.ebi.ac.uk/pdbsum/7s9a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s9a ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bavi N]]
		+	[[Category: Clark MD]]
		+	[[Category: Contreras GF]]
		+	[[Category: Milewski W]]
		+	[[Category: Perozo E]]
		+	[[Category: Reddy BG]]
		+	[[Category: Shen R]]

OCA at 12:40, 27 October 2021

OCA — Wed, 27 Oct 2021 12:40:50 GMT

←Older revision		Revision as of 12:40, 27 October 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7s9a is ON HOLD	+	The entry 7s9a is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7s9a" [edit=sysop:move=sysop]

OCA — Wed, 29 Sep 2021 07:48:17 GMT

Protected "7s9a" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:48, 29 September 2021

OCA: New page: '''Unreleased structure''' The entry 7s9a is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Sep 2021 07:48:16 GMT

New page: '''Unreleased structure''' The entry 7s9a is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7s9a is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7s9a - Revision history

OCA at 17:02, 15 December 2021

OCA at 14:33, 3 November 2021

OCA at 12:40, 27 October 2021

OCA: Protected "7s9a" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7s9a is ON HOLD Authors: Description: Category: Unreleased Structures