7sen - Revision history

OCA at 16:50, 18 October 2023

OCA — Wed, 18 Oct 2023 16:50:09 GMT

←Older revision		Revision as of 16:50, 18 October 2023
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-	====	+	==Crystal structure of Fab containing a fluorescent noncanonical amino acid with blocked excited state proton transfer==
-	<StructureSection load='7sen' size='340' side='right'caption='[[7sen]]' scene=''>	+	<StructureSection load='7sen' size='340' side='right'caption='[[7sen]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7sen]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SEN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SEN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sen FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sen OCA], [https://pdbe.org/7sen PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sen RCSB], [https://www.ebi.ac.uk/pdbsum/7sen PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sen ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.09Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DV7:L-(7-hydroxycoumarin-4-yl)ethylglycine'>DV7</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sen FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sen OCA], [https://pdbe.org/7sen PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sen RCSB], [https://www.ebi.ac.uk/pdbsum/7sen PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sen ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The fluorescent non-canonical amino acid (fNCAA) L-(7-hydroxycoumarin-4-yl)ethylglycine (7-HCAA) contains a photoacidic 7-hydroxycoumarin (7-HC) side chain whose fluorescence properties can be tuned by its environment. In proteins, many alterations to 7-HCAA's fluorescence spectra have been reported including increases and decreases in intensity and red- and blue-shifted emission maxima. The ability to rationally design protein environments that alter 7-HCAA's fluorescence properties in predictable ways could lead to novel protein-based sensors of biological function. However, these efforts are likely limited by a lack of structural characterization of 7-HCAA-containing proteins. Here, we report the steady-state spectroscopic and x-ray crystallographic characterization of a 7-HCAA-containing antibody fragment (in the apo and antigen-bound forms) in which a substantially blue-shifted 7-HCAA emission maximum ( approximately 70 nm) is observed relative to the free amino acid. Our structural characterization of these proteins provides evidence that the blue shift is a consequence of the fact that excited state proton transfer (ESPT) from the 7-HC phenol has been almost completely blocked by interactions with the protein backbone. Furthermore, a direct interaction between a residue in the antigen and the fluorophore served to further block proton transfer relative to the apoprotein. The structural basis of the unprecedented blue shift in 7-HCAA emission reported here provides a framework for the development of new fluorescent protein-based sensors.
		+
		+	Structural Basis for Blocked Excited State Proton Transfer in a Fluorescent, Photoacidic Non-Canonical Amino Acid-Containing Antibody Fragment.,Henderson JN, Simmons CR, Mills JH J Mol Biol. 2022 Apr 30;434(8):167455. doi: 10.1016/j.jmb.2022.167455. Epub 2022 , Jan 13. PMID:35033559<ref>PMID:35033559</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7sen" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Henderson JN]]
		+	[[Category: Mills JH]]
		+	[[Category: Simmons CR]]

OCA at 07:03, 2 February 2022

OCA — Wed, 02 Feb 2022 07:03:27 GMT

←Older revision		Revision as of 07:03, 2 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7sen ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7sen' size='340' side='right'caption='[[7sen]]' scene=''>
-	~~Authors~~: ~~Henderson, J~~.N.~~, Mills, J~~.H.~~, Simmons, C~~.R.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of Fab containing a fluorescent noncanonical amino acid with blocked excited state proton transfer~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sen FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sen OCA], [https://pdbe.org/7sen PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sen RCSB], [https://www.ebi.ac.uk/pdbsum/7sen PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sen ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Simmons~~, C.R]]	+	__TOC__
-	[[Category: ~~Mills, J.H~~]]	+	</StructureSection>
-	[[Category: ~~Henderson, J.N~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 10:25, 13 October 2021

OCA — Wed, 13 Oct 2021 10:25:02 GMT

←Older revision		Revision as of 10:25, 13 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7sen is ON HOLD	+	The entry 7sen is ON HOLD until Paper Publication

	Authors: Henderson, J.N., Mills, J.H., Simmons, C.R.		Authors: Henderson, J.N., Mills, J.H., Simmons, C.R.

-	Description: Crystal structure of Fab containing a fluorescent noncanonical amino acid with ~~block~~ excited state proton transfer.	+	Description: Crystal structure of Fab containing a fluorescent noncanonical amino acid with blocked excited state proton transfer
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Simmons, C.R]]		[[Category: Simmons, C.R]]
	[[Category: Mills, J.H]]		[[Category: Mills, J.H]]
	[[Category: Henderson, J.N]]		[[Category: Henderson, J.N]]

OCA: Protected "7sen" [edit=sysop:move=sysop]

OCA — Wed, 06 Oct 2021 04:19:34 GMT

Protected "7sen" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:19, 6 October 2021

OCA: New page: '''Unreleased structure''' The entry 7sen is ON HOLD Authors: Henderson, J.N., Mills, J.H., Simmons, C.R. Description: Crystal structure of Fab containing a fluorescent noncanonical am...

OCA — Wed, 06 Oct 2021 04:19:33 GMT

New page: '''Unreleased structure''' The entry 7sen is ON HOLD Authors: Henderson, J.N., Mills, J.H., Simmons, C.R. Description: Crystal structure of Fab containing a fluorescent noncanonical am...

New page

'''Unreleased structure'''

The entry 7sen is ON HOLD

Authors: Henderson, J.N., Mills, J.H., Simmons, C.R.

Description: Crystal structure of Fab containing a fluorescent noncanonical amino acid with block excited state proton transfer.
[[Category: Unreleased Structures]]
[[Category: Simmons, C.R]]
[[Category: Mills, J.H]]
[[Category: Henderson, J.N]]