7shj - Revision history

OCA at 07:20, 3 April 2024

OCA — Wed, 03 Apr 2024 07:20:57 GMT

←Older revision		Revision as of 07:20, 3 April 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7shj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SHJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[7shj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SHJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.13Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7shj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7shj OCA], [https://pdbe.org/7shj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7shj RCSB], [https://www.ebi.ac.uk/pdbsum/7shj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7shj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7shj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7shj OCA], [https://pdbe.org/7shj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7shj RCSB], [https://www.ebi.ac.uk/pdbsum/7shj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7shj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A6B2IEQ3_ACIBA A0A6B2IEQ3_ACIBA]		[https://www.uniprot.org/uniprot/A0A6B2IEQ3_ACIBA A0A6B2IEQ3_ACIBA]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Acinetobacter baumannii is a Gram-negative nosocomial pathogen associated with significant disease. Crucial to the survival and pathogenesis of A. baumannii is the ability to acquire essential micronutrients such as Zn(II). Recruitment of Zn(II) by A. baumannii is mediated, at least in part, by the periplasmic solute-binding protein ZnuA and the ATP-binding cassette transporter ZnuBC. Here, we combined genomic, biochemical, and structural approaches to characterize A. baumannii AB5075_UW ZnuA. Bioinformatic analyses using a diverse collection of A. baumannii genomes determined that ZnuA is highly conserved, with the binding site comprised by three strictly conserved histidine residues. The structure of metal-free ZnuA was determined at 2.1 A resolution, with molecular dynamics analyses revealing loop alpha2beta2, which harbors the putative Zn(II)-coordinating residue His41, to be highly mobile in the metal-free state. The contribution of the putative binding site histidine residues to Zn(II) interaction was further probed by mutagenesis. Analysis of ZnuA mutant variants was performed by quantitative metal binding assays, differential scanning fluorimetry, and affinity measurements, which showed that all three histidine residues contributed to Zn(II)-recruitment, albeit to different extents. Collectively, these analyses provide insight into the mechanism of Zn(II)-binding by A. baumannii ZnuA and expand our understanding of the functional diversity of Zn(II)-recruiting proteins.
-
-	Structural and biochemical characterization of Acinetobacter baumannii ZnuA.,Alquethamy S, Ganio K, Luo Z, Hossain SI, Hayes AJ, Ve T, Davies MR, Deplazes E, Kobe B, McDevitt CA J Inorg Biochem. 2022 Jun;231:111787. doi: 10.1016/j.jinorgbio.2022.111787. Epub , 2022 Mar 8. PMID:35303613<ref>PMID:35303613</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 7shj" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:03, 10 May 2023

OCA — Wed, 10 May 2023 07:03:39 GMT

←Older revision		Revision as of 07:03, 10 May 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7shj is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of Acinetobacter baumannii ZnuA in the metal-free state==
		+	<StructureSection load='7shj' size='340' side='right'caption='[[7shj]], [[Resolution\|resolution]] 2.13Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7shj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SHJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7shj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7shj OCA], [https://pdbe.org/7shj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7shj RCSB], [https://www.ebi.ac.uk/pdbsum/7shj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7shj ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A6B2IEQ3_ACIBA A0A6B2IEQ3_ACIBA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acinetobacter baumannii is a Gram-negative nosocomial pathogen associated with significant disease. Crucial to the survival and pathogenesis of A. baumannii is the ability to acquire essential micronutrients such as Zn(II). Recruitment of Zn(II) by A. baumannii is mediated, at least in part, by the periplasmic solute-binding protein ZnuA and the ATP-binding cassette transporter ZnuBC. Here, we combined genomic, biochemical, and structural approaches to characterize A. baumannii AB5075_UW ZnuA. Bioinformatic analyses using a diverse collection of A. baumannii genomes determined that ZnuA is highly conserved, with the binding site comprised by three strictly conserved histidine residues. The structure of metal-free ZnuA was determined at 2.1 A resolution, with molecular dynamics analyses revealing loop alpha2beta2, which harbors the putative Zn(II)-coordinating residue His41, to be highly mobile in the metal-free state. The contribution of the putative binding site histidine residues to Zn(II) interaction was further probed by mutagenesis. Analysis of ZnuA mutant variants was performed by quantitative metal binding assays, differential scanning fluorimetry, and affinity measurements, which showed that all three histidine residues contributed to Zn(II)-recruitment, albeit to different extents. Collectively, these analyses provide insight into the mechanism of Zn(II)-binding by A. baumannii ZnuA and expand our understanding of the functional diversity of Zn(II)-recruiting proteins.

-	~~Authors: Luo~~, Z., ~~McDevitt~~, ~~C.A.~~, ~~Kobe~~, B.	+	Structural and biochemical characterization of Acinetobacter baumannii ZnuA.,Alquethamy S, Ganio K, Luo Z, Hossain SI, Hayes AJ, Ve T, Davies MR, Deplazes E, Kobe B, McDevitt CA J Inorg Biochem. 2022 Jun;231:111787. doi: 10.1016/j.jinorgbio.2022.111787. Epub , 2022 Mar 8. PMID:35303613<ref>PMID:35303613</ref>

-	~~Description: Crystal structure~~ of ~~Acinetobacter baumannii ZnuA in~~ the ~~metal~~-~~free state~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Kobe, B]]	+	<div class="pdbe-citations 7shj" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Mcdevitt, C.A~~]]	+	== References ==
-	[[Category: ~~Luo, Z~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Acinetobacter baumannii]]
		+	[[Category: Large Structures]]
		+	[[Category: Kobe B]]
		+	[[Category: Luo Z]]
		+	[[Category: McDevitt CA]]

OCA at 11:59, 29 December 2021

OCA — Wed, 29 Dec 2021 11:59:47 GMT

←Older revision		Revision as of 11:59, 29 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7shj is ON HOLD	+	The entry 7shj is ON HOLD until Paper Publication

	Authors: Luo, Z., McDevitt, C.A., Kobe, B.		Authors: Luo, Z., McDevitt, C.A., Kobe, B.
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	Description: Crystal structure of Acinetobacter baumannii ZnuA in the metal-free state		Description: Crystal structure of Acinetobacter baumannii ZnuA in the metal-free state
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Mcdevitt, C.A]]
	[[Category: Kobe, B]]		[[Category: Kobe, B]]
		+	[[Category: Mcdevitt, C.A]]
	[[Category: Luo, Z]]		[[Category: Luo, Z]]

OCA: Protected "7shj" [edit=sysop:move=sysop]

OCA — Wed, 20 Oct 2021 15:07:53 GMT

Protected "7shj" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:07, 20 October 2021

OCA: New page: '''Unreleased structure''' The entry 7shj is ON HOLD Authors: Luo, Z., McDevitt, C.A., Kobe, B. Description: Crystal structure of Acinetobacter baumannii ZnuA in the metal-free state [...

OCA — Wed, 20 Oct 2021 15:07:52 GMT

New page: '''Unreleased structure''' The entry 7shj is ON HOLD Authors: Luo, Z., McDevitt, C.A., Kobe, B. Description: Crystal structure of Acinetobacter baumannii ZnuA in the metal-free state [...

New page

'''Unreleased structure'''

The entry 7shj is ON HOLD

Authors: Luo, Z., McDevitt, C.A., Kobe, B.

Description: Crystal structure of Acinetobacter baumannii ZnuA in the metal-free state
[[Category: Unreleased Structures]]
[[Category: Mcdevitt, C.A]]
[[Category: Kobe, B]]
[[Category: Luo, Z]]