OCA at 16:51, 18 October 2023

OCA — Wed, 18 Oct 2023 16:51:34 GMT

←Older revision		Revision as of 16:51, 18 October 2023
Line 1:		Line 1:

-	====	+	==IgE-Fc in complex with omalizumab variant C02==
-	<StructureSection load='7shu' size='340' side='right'caption='[[7shu]]' scene=''>	+	<StructureSection load='7shu' size='340' side='right'caption='[[7shu]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7shu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SHU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SHU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7shu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7shu OCA], [https://pdbe.org/7shu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7shu RCSB], [https://www.ebi.ac.uk/pdbsum/7shu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7shu ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.75Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7shu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7shu OCA], [https://pdbe.org/7shu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7shu RCSB], [https://www.ebi.ac.uk/pdbsum/7shu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7shu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IGHE_HUMAN IGHE_HUMAN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Antibody drugs exert therapeutic effects via a range of mechanisms, including competitive inhibition, allosteric modulation, and immune effector mechanisms. Facilitated dissociation is an additional mechanism where antibody-mediated "disruption" of stable high-affinity macromolecular complexes can potentially enhance therapeutic efficacy. However, this mechanism is not well understood or utilized therapeutically. Here, we investigate and engineer the weak disruptive activity of an existing therapeutic antibody, omalizumab, which targets IgE antibodies to block the allergic response. We develop a yeast display approach to select for and engineer antibody disruptive efficiency and generate potent omalizumab variants that dissociate receptor-bound IgE. We determine a low resolution cryo-EM structure of a transient disruption intermediate containing the IgE-Fc, its partially dissociated receptor and an antibody inhibitor. Our results provide a conceptual framework for engineering disruptive inhibitors for other targets, insights into the failure in clinical trials of the previous high affinity omalizumab HAE variant and anti-IgE antibodies that safely and rapidly disarm allergic effector cells.
		+
		+	Directed evolution of and structural insights into antibody-mediated disruption of a stable receptor-ligand complex.,Pennington LF, Gasser P, Kleinboelting S, Zhang C, Skiniotis G, Eggel A, Jardetzky TS Nat Commun. 2021 Dec 3;12(1):7069. doi: 10.1038/s41467-021-27397-z. PMID:34862384<ref>PMID:34862384</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7shu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Jardetzky TJ]]
		+	[[Category: Kleinboelting S]]
		+	[[Category: Pennington LF]]

OCA at 16:50, 15 December 2021

OCA — Wed, 15 Dec 2021 16:50:43 GMT

←Older revision		Revision as of 16:50, 15 December 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7shu ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7shu' size='340' side='right'caption='[[7shu]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7shu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7shu OCA], [https://pdbe.org/7shu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7shu RCSB], [https://www.ebi.ac.uk/pdbsum/7shu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7shu ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 12:11, 17 November 2021

OCA — Wed, 17 Nov 2021 12:11:35 GMT

←Older revision		Revision as of 12:11, 17 November 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7shu is ON HOLD	+	The entry 7shu is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7shu" [edit=sysop:move=sysop]

OCA — Wed, 20 Oct 2021 15:08:35 GMT

Protected "7shu" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:08, 20 October 2021

OCA: New page: '''Unreleased structure''' The entry 7shu is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 Oct 2021 15:08:33 GMT

New page: '''Unreleased structure''' The entry 7shu is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7shu is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7shu - Revision history

OCA at 16:51, 18 October 2023

OCA at 16:50, 15 December 2021

OCA at 12:11, 17 November 2021

OCA: Protected "7shu" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7shu is ON HOLD Authors: Description: Category: Unreleased Structures