7sog - Revision history

OCA at 16:53, 18 October 2023

OCA — Wed, 18 Oct 2023 16:53:38 GMT

←Older revision		Revision as of 16:53, 18 October 2023
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	==Thermostable actophorin==		==Thermostable actophorin==
-	<StructureSection load='7sog' size='340' side='right'caption='[[7sog]]' scene=''>	+	<StructureSection load='7sog' size='340' side='right'caption='[[7sog]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SOG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SOG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7sog]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acanthamoeba_castellanii Acanthamoeba castellanii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SOG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SOG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sog OCA], [https://pdbe.org/7sog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sog RCSB], [https://www.ebi.ac.uk/pdbsum/7sog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sog ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.74Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sog OCA], [https://pdbe.org/7sog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sog RCSB], [https://www.ebi.ac.uk/pdbsum/7sog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sog ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ACTP_ACACA ACTP_ACACA] Forms a one to one complex with monomeric actin. Can regulate the pool available for polymerization. Severs actin filaments in a dose-dependent manner.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Actophorin, which was recently tested for crystallization under microgravity on the International Space Station, was subjected to mutagenesis to identify a construct with improved biophysical properties that were expected to improve the extent of diffraction. First, 20 mutations, including one C-terminal deletion of three residues, were introduced individually into actophorin, resulting in modest increases in thermal stability of between +0.5 degrees C and +2.2 degrees C. All but two of the stabilizing mutants increased both the rates of severing F-actin filaments and of spontaneous polymerization of pyrenyl G-actin in vitro. When the individual mutations were combined into a single actophorin variant, Acto-2, the overall thermal stability was 22 degrees C higher than that of wild-type actophorin. When an inactivating S2P mutation in Acto-2 was restored, Acto-2/P2S was more stable by 20 degrees C but was notably more active than the wild-type protein. The inactivating S2P mutation reaffirms the importance that Ser2 plays in the F-actin-severing reaction. The crystal structure of Acto-2 was solved to 1.7 A resolution in a monoclinic space group, a first for actophorin. Surprisingly, despite the increase in thermal stability, the extended beta-turn region, which is intimately involved in interactions with F-actin, is disordered in one copy of Acto-2 in the asymmetric unit. These observations emphasize the complex interplay among protein thermal stability, function and dynamics.
		+
		+	Structure and activity of a thermally stable mutant of Acanthamoeba actophorin.,Quirk S, Lieberman RL Acta Crystallogr F Struct Biol Commun. 2022 Apr 1;78(Pt 4):150-160. doi:, 10.1107/S2053230X22002448. Epub 2022 Mar 28. PMID:35400667<ref>PMID:35400667</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7sog" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Acanthamoeba castellanii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lieberman RL]]		[[Category: Lieberman RL]]
	[[Category: Quirk S]]		[[Category: Quirk S]]

OCA at 02:56, 21 April 2022

OCA — Thu, 21 Apr 2022 02:56:15 GMT

←Older revision		Revision as of 02:56, 21 April 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7sog ~~is ON HOLD until Paper Publication~~	+	==Thermostable actophorin==
-		+	<StructureSection load='7sog' size='340' side='right'caption='[[7sog]]' scene=''>
-	~~Authors~~: ~~Lieberman~~, R.L., ~~Quirk~~, S.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SOG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SOG FirstGlance]. <br>
-	~~Description: Thermostable actophorin~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sog OCA], [https://pdbe.org/7sog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sog RCSB], [https://www.ebi.ac.uk/pdbsum/7sog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sog ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Lieberman~~, R.L~~]]	+	__TOC__
-	[[Category: Quirk, S]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lieberman RL]]
		+	[[Category: Quirk S]]

OCA at 08:32, 8 December 2021

OCA — Wed, 08 Dec 2021 08:32:46 GMT

←Older revision		Revision as of 08:32, 8 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7sog is ON HOLD	+	The entry 7sog is ON HOLD until Paper Publication

	Authors: Lieberman, R.L., Quirk, S.		Authors: Lieberman, R.L., Quirk, S.

OCA: Protected "7sog" [edit=sysop:move=sysop]

OCA — Wed, 10 Nov 2021 05:16:47 GMT

Protected "7sog" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:16, 10 November 2021

OCA: New page: '''Unreleased structure''' The entry 7sog is ON HOLD Authors: Lieberman, R.L., Quirk, S. Description: Thermostable actophorin Category: Unreleased Structures [[Category: Lieberman,...

OCA — Wed, 10 Nov 2021 05:16:46 GMT

New page: '''Unreleased structure''' The entry 7sog is ON HOLD Authors: Lieberman, R.L., Quirk, S. Description: Thermostable actophorin Category: Unreleased Structures [[Category: Lieberman,...

New page

'''Unreleased structure'''

The entry 7sog is ON HOLD

Authors: Lieberman, R.L., Quirk, S.

Description: Thermostable actophorin
[[Category: Unreleased Structures]]
[[Category: Lieberman, R.L]]
[[Category: Quirk, S]]