7spz - Revision history

OCA at 16:54, 18 October 2023

OCA — Wed, 18 Oct 2023 16:54:08 GMT

←Older revision		Revision as of 16:54, 18 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7spz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_lamblia_ATCC_50803 Giardia lamblia ATCC 50803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SPZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[7spz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_lamblia_ATCC_50803 Giardia lamblia ATCC 50803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SPZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7spz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7spz OCA], [https://pdbe.org/7spz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7spz RCSB], [https://www.ebi.ac.uk/pdbsum/7spz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7spz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7spz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7spz OCA], [https://pdbe.org/7spz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7spz RCSB], [https://www.ebi.ac.uk/pdbsum/7spz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7spz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ASNA_GIAIC ASNA_GIAIC]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.	+	[https://www.uniprot.org/uniprot/ASNA_GIAIC ASNA_GIAIC] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 04:59, 8 September 2022

OCA — Thu, 08 Sep 2022 04:59:06 GMT

←Older revision		Revision as of 04:59, 8 September 2022
Line 1:		Line 1:

	==Nucleotide-free Get3 in two open forms==		==Nucleotide-free Get3 in two open forms==
-	<StructureSection load='7spz' size='340' side='right'caption='[[7spz]]' scene=''>	+	<StructureSection load='7spz' size='340' side='right'caption='[[7spz]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SPZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7spz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_lamblia_ATCC_50803 Giardia lamblia ATCC 50803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SPZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7spz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7spz OCA], [https://pdbe.org/7spz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7spz RCSB], [https://www.ebi.ac.uk/pdbsum/7spz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7spz ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7spz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7spz OCA], [https://pdbe.org/7spz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7spz RCSB], [https://www.ebi.ac.uk/pdbsum/7spz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7spz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/ASNA_GIAIC ASNA_GIAIC]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tail-anchored (TA) membrane proteins, accounting for roughly 2% of proteomes, are primarily targeted posttranslationally to the endoplasmic reticulum membrane by the guided entry of TA proteins (GET) pathway. For this complicated process, it remains unknown how the central targeting factor Get3 uses nucleotide to facilitate large conformational changes to recognize then bind clients while also preventing exposure of hydrophobic surfaces. Here, we identify the GET pathway in Giardia intestinalis and present the structure of the Get3-client complex in the critical postnucleotide-hydrolysis state, demonstrating that Get3 reorganizes the client-binding domain (CBD) to accommodate and shield the client transmembrane helix. Four additional structures of GiGet3, spanning the nucleotide-free (apo) open to closed transition and the ATP-bound state, reveal the details of nucleotide stabilization and occluded CBD. This work resolves key conundrums and allows for a complete model of the dramatic conformational landscape of Get3.
		+
		+	Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3.,Fry MY, Najdrova V, Maggiolo AO, Saladi SM, Dolezal P, Clemons WM Jr Nat Struct Mol Biol. 2022 Jul 18. pii: 10.1038/s41594-022-00798-4. doi:, 10.1038/s41594-022-00798-4. PMID:35851188<ref>PMID:35851188</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7spz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Giardia lamblia ATCC 50803]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Clemons Jr WM]]		[[Category: Clemons Jr WM]]
	[[Category: Fry MY]]		[[Category: Fry MY]]
	[[Category: Maggiolo AO]]		[[Category: Maggiolo AO]]

OCA at 11:07, 31 August 2022

OCA — Wed, 31 Aug 2022 11:07:46 GMT

←Older revision		Revision as of 11:07, 31 August 2022
Line 1:		Line 1:

	==Nucleotide-free Get3 in two open forms==		==Nucleotide-free Get3 in two open forms==
-	<StructureSection load='7spz' size='340' side='right'caption='[[7spz]]~~, [[Resolution\|resolution]] 3.00Å~~' scene=''>	+	<StructureSection load='7spz' size='340' side='right'caption='[[7spz]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[7spz]] is a 2 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SPZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SPZ FirstGlance]. <br>
-	</td></tr>~~<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7spz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7spz OCA], [https://pdbe.org/7spz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7spz RCSB], [https://www.ebi.ac.uk/pdbsum/7spz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7spz ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7spz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7spz OCA], [https://pdbe.org/7spz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7spz RCSB], [https://www.ebi.ac.uk/pdbsum/7spz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7spz ProSAT]</span></td></tr>	+
	</table>		</table>
-	== Function ==
-	[[https://www.uniprot.org/uniprot/ASNA_GIAIC ASNA_GIAIC]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Tail-anchored (TA) membrane proteins, accounting for roughly 2% of proteomes, are primarily targeted posttranslationally to the endoplasmic reticulum membrane by the guided entry of TA proteins (GET) pathway. For this complicated process, it remains unknown how the central targeting factor Get3 uses nucleotide to facilitate large conformational changes to recognize then bind clients while also preventing exposure of hydrophobic surfaces. Here, we identify the GET pathway in Giardia intestinalis and present the structure of the Get3-client complex in the critical postnucleotide-hydrolysis state, demonstrating that Get3 reorganizes the client-binding domain (CBD) to accommodate and shield the client transmembrane helix. Four additional structures of GiGet3, spanning the nucleotide-free (apo) open to closed transition and the ATP-bound state, reveal the details of nucleotide stabilization and occluded CBD. This work resolves key conundrums and allows for a complete model of the dramatic conformational landscape of Get3.
-
-	Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3.,Fry MY, Najdrova V, Maggiolo AO, Saladi SM, Dolezal P, Clemons WM Jr Nat Struct Mol Biol. 2022 Jul 18. pii: 10.1038/s41594-022-00798-4. doi:, 10.1038/s41594-022-00798-4. PMID:35851188<ref>PMID:35851188</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 7spz" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Clemons~~, W M~~]]	+	[[Category: Clemons Jr WM]]
-	[[Category: Fry~~, M Y~~]]	+	[[Category: Fry MY]]
-	[[Category: Maggiolo~~, A O]]~~	+	[[Category: Maggiolo AO]]
-	~~[[Category: Chaperone]]~~	+
-	~~[[Category: Tail-anchored membrane protein targeting deviant walker a atpase targeting factor~~]]	+

OCA at 05:11, 10 August 2022

OCA — Wed, 10 Aug 2022 05:11:49 GMT

←Older revision		Revision as of 05:11, 10 August 2022
Line 1:		Line 1:

	==Nucleotide-free Get3 in two open forms==		==Nucleotide-free Get3 in two open forms==
-	<StructureSection load='7spz' size='340' side='right'caption='[[7spz]]' scene=''>	+	<StructureSection load='7spz' size='340' side='right'caption='[[7spz]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SPZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7spz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SPZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7spz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7spz OCA], [https://pdbe.org/7spz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7spz RCSB], [https://www.ebi.ac.uk/pdbsum/7spz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7spz ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7spz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7spz OCA], [https://pdbe.org/7spz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7spz RCSB], [https://www.ebi.ac.uk/pdbsum/7spz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7spz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/ASNA_GIAIC ASNA_GIAIC]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tail-anchored (TA) membrane proteins, accounting for roughly 2% of proteomes, are primarily targeted posttranslationally to the endoplasmic reticulum membrane by the guided entry of TA proteins (GET) pathway. For this complicated process, it remains unknown how the central targeting factor Get3 uses nucleotide to facilitate large conformational changes to recognize then bind clients while also preventing exposure of hydrophobic surfaces. Here, we identify the GET pathway in Giardia intestinalis and present the structure of the Get3-client complex in the critical postnucleotide-hydrolysis state, demonstrating that Get3 reorganizes the client-binding domain (CBD) to accommodate and shield the client transmembrane helix. Four additional structures of GiGet3, spanning the nucleotide-free (apo) open to closed transition and the ATP-bound state, reveal the details of nucleotide stabilization and occluded CBD. This work resolves key conundrums and allows for a complete model of the dramatic conformational landscape of Get3.
		+
		+	Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3.,Fry MY, Najdrova V, Maggiolo AO, Saladi SM, Dolezal P, Clemons WM Jr Nat Struct Mol Biol. 2022 Jul 18. pii: 10.1038/s41594-022-00798-4. doi:, 10.1038/s41594-022-00798-4. PMID:35851188<ref>PMID:35851188</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7spz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Clemons ~~Jr WM~~]]	+	[[Category: Clemons, W M]]
-	[[Category: Fry MY]]	+	[[Category: Fry, M Y]]
-	[[Category: Maggiolo AO]]	+	[[Category: Maggiolo, A O]]
		+	[[Category: Chaperone]]
		+	[[Category: Tail-anchored membrane protein targeting deviant walker a atpase targeting factor]]

OCA at 06:51, 20 July 2022

OCA — Wed, 20 Jul 2022 06:51:48 GMT

←Older revision		Revision as of 06:51, 20 July 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7spz is ~~ON HOLD until Paper Publication~~	+	==Nucleotide-free Get3 in two open forms==
-		+	<StructureSection load='7spz' size='340' side='right'caption='[[7spz]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SPZ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7spz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7spz OCA], [https://pdbe.org/7spz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7spz RCSB], [https://www.ebi.ac.uk/pdbsum/7spz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7spz ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Clemons Jr WM]]
		+	[[Category: Fry MY]]
		+	[[Category: Maggiolo AO]]

OCA: Protected "7spz" [edit=sysop:move=sysop]

OCA — Wed, 10 Nov 2021 05:19:10 GMT

Protected "7spz" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:19, 10 November 2021

OCA: New page: '''Unreleased structure''' The entry 7spz is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 10 Nov 2021 05:19:09 GMT

New page: '''Unreleased structure''' The entry 7spz is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7spz is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]