7sux - Revision history

OCA at 16:55, 18 October 2023

OCA — Wed, 18 Oct 2023 16:55:07 GMT

←Older revision		Revision as of 16:55, 18 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7sux]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sorghum_bicolor Sorghum bicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SUX FirstGlance]. <br>		<table><tr><td colspan='2'>[[7sux]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sorghum_bicolor Sorghum bicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SUX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.36Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sux OCA], [https://pdbe.org/7sux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sux RCSB], [https://www.ebi.ac.uk/pdbsum/7sux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sux ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sux OCA], [https://pdbe.org/7sux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sux RCSB], [https://www.ebi.ac.uk/pdbsum/7sux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sux ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NCPR_SORBI NCPR_SORBI]] This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.[HAMAP-Rule:MF_03212]<ref>PMID:32332088</ref>	+	[https://www.uniprot.org/uniprot/NCPR_SORBI NCPR_SORBI] This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.[HAMAP-Rule:MF_03212]<ref>PMID:32332088</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7sux" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7sux" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[NADPH-Cytochrome P450 Reductase\|NADPH-Cytochrome P450 Reductase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:25, 7 September 2022

OCA — Wed, 07 Sep 2022 19:25:12 GMT

←Older revision		Revision as of 19:25, 7 September 2022
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	==NADPH-dependent cytochrome P450 reductase 2b from Sorghum bicolor (SbCPR2b) -NADP+ complex==		==NADPH-dependent cytochrome P450 reductase 2b from Sorghum bicolor (SbCPR2b) -NADP+ complex==
-	<StructureSection load='7sux' size='340' side='right'caption='[[7sux]]' scene=''>	+	<StructureSection load='7sux' size='340' side='right'caption='[[7sux]], [[Resolution\|resolution]] 2.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SUX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7sux]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sorghum_bicolor Sorghum bicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SUX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sux OCA], [https://pdbe.org/7sux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sux RCSB], [https://www.ebi.ac.uk/pdbsum/7sux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sux ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sux OCA], [https://pdbe.org/7sux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sux RCSB], [https://www.ebi.ac.uk/pdbsum/7sux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sux ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/NCPR_SORBI NCPR_SORBI]] This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.[HAMAP-Rule:MF_03212]<ref>PMID:32332088</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Plant NADPH-dependent cytochrome P450 reductase (CPR) is a multidomain enzyme that donates electrons for hydroxylation reactions catalyzed by class II cytochrome P450 monooxygenases involved in the synthesis of many primary and secondary metabolites. These P450 enzymes include trans-cinnamate-4-hydroxylase, p-coumarate-3'-hydroxylase, and ferulate-5-hydroxylase involved in monolignol biosynthesis. Because of its role in monolignol biosynthesis, alterations in CPR activity could change the composition and overall output of lignin. Therefore, to understand the structure and function of three CPR subunits from sorghum, recombinant subunits SbCPR2a, SbCPR2b, and SbCPR2c were subjected to X-ray crystallography and kinetic assays. Steady-state kinetic analyses demonstrated that all three CPR subunits supported the oxidation reactions catalyzed by SbC4H1 (CYP73A33) and SbC3'H (CYP98A1). Furthermore, comparing the SbCPR2b structure with the well-investigated CPRs from mammals enabled us to identify critical residues of functional importance and suggested that the plant flavin mononucleotide-binding domain might be more flexible than mammalian homologs. In addition, the elucidated structure of SbCPR2b included the first observation of NADP(+) in a native CPR. Overall, we conclude that the connecting domain of SbCPR2, especially its hinge region, could serve as a target to alter biomass composition in bioenergy and forage sorghums through protein engineering.
		+
		+	Functional and structural insight into the flexibility of cytochrome P450 reductases from Sorghum bicolor and its implications for lignin composition.,Zhang B, Munske GR, Timokhin VI, Ralph J, Davydov DR, Vermerris W, Sattler SE, Kang C J Biol Chem. 2022 Apr;298(4):101761. doi: 10.1016/j.jbc.2022.101761. Epub 2022, Feb 21. PMID:35202651<ref>PMID:35202651</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7sux" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Sorghum bicolor]]
	[[Category: Kang C]]		[[Category: Kang C]]
	[[Category: Zhang B]]		[[Category: Zhang B]]

OCA at 06:36, 31 August 2022

OCA — Wed, 31 Aug 2022 06:36:33 GMT

←Older revision		Revision as of 06:36, 31 August 2022
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-	'''Unreleased structure'''

-	~~The entry 7sux is ON HOLD until Paper Publication~~	+	==NADPH-dependent cytochrome P450 reductase 2b from Sorghum bicolor (SbCPR2b) -NADP+ complex==
-		+	<StructureSection load='7sux' size='340' side='right'caption='[[7sux]]' scene=''>
-	~~Authors: Zhang, B., Kang, C.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SUX FirstGlance]. <br>
-	~~Description:~~ NADPH-dependent cytochrome P450 reductase 2b from Sorghum bicolor (SbCPR2b) -NADP+ complex	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sux OCA], [https://pdbe.org/7sux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sux RCSB], [https://www.ebi.ac.uk/pdbsum/7sux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sux ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Zhang~~, B]]	+	__TOC__
-	[[Category: Kang, C]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kang C]]
		+	[[Category: Zhang B]]

OCA at 09:16, 5 January 2022

OCA — Wed, 05 Jan 2022 09:16:49 GMT

←Older revision		Revision as of 09:16, 5 January 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7sux is ON HOLD	+	The entry 7sux is ON HOLD until Paper Publication

	Authors: Zhang, B., Kang, C.		Authors: Zhang, B., Kang, C.

OCA at 08:34, 8 December 2021

OCA — Wed, 08 Dec 2021 08:34:09 GMT

←Older revision		Revision as of 08:34, 8 December 2021
Line 5:		Line 5:
	Authors: Zhang, B., Kang, C.		Authors: Zhang, B., Kang, C.

-	Description: NADPH-dependent cytochrome P450 reductase 2b from ~~sorghum~~ bicolor (SbCPR2b) -NADP+ complex	+	Description: NADPH-dependent cytochrome P450 reductase 2b from Sorghum bicolor (SbCPR2b) -NADP+ complex
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Zhang, B]]		[[Category: Zhang, B]]
	[[Category: Kang, C]]		[[Category: Kang, C]]

OCA: Protected "7sux" [edit=sysop:move=sysop]

OCA — Wed, 24 Nov 2021 11:01:47 GMT

Protected "7sux" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:01, 24 November 2021

OCA: New page: '''Unreleased structure''' The entry 7sux is ON HOLD Authors: Zhang, B., Kang, C. Description: NADPH-dependent cytochrome P450 reductase 2b from sorghum bicolor (SbCPR2b) -NADP+ comple...

OCA — Wed, 24 Nov 2021 11:01:46 GMT

New page: '''Unreleased structure''' The entry 7sux is ON HOLD Authors: Zhang, B., Kang, C. Description: NADPH-dependent cytochrome P450 reductase 2b from sorghum bicolor (SbCPR2b) -NADP+ comple...

New page

'''Unreleased structure'''

The entry 7sux is ON HOLD

Authors: Zhang, B., Kang, C.

Description: NADPH-dependent cytochrome P450 reductase 2b from sorghum bicolor (SbCPR2b) -NADP+ complex
[[Category: Unreleased Structures]]
[[Category: Zhang, B]]
[[Category: Kang, C]]