7sv6 - Revision history

OCA at 16:55, 18 October 2023

OCA — Wed, 18 Oct 2023 16:55:19 GMT

←Older revision		Revision as of 16:55, 18 October 2023
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	<StructureSection load='7sv6' size='340' side='right'caption='[[7sv6]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='7sv6' size='340' side='right'caption='[[7sv6]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7sv6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SV6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7sv6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenibacillus_alvei Paenibacillus alvei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SV6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D2Y:methyl+2-acetamido-4-O-{2-acetamido-4,6-O-[(1S)-1-carboxyethylidene]-2-deoxy-beta-D-mannopyranosyl}-2-deoxy-beta-D-glucopyranoside'>D2Y</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7sv3\|7sv3]], [[7sv5\|7sv5]], [[7sv4\|7sv4]]</div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D2Y:methyl+2-acetamido-4-O-{2-acetamido-4,6-O-[(1S)-1-carboxyethylidene]-2-deoxy-beta-D-mannopyranosyl}-2-deoxy-beta-D-glucopyranoside'>D2Y</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sv6 OCA], [https://pdbe.org/7sv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sv6 RCSB], [https://www.ebi.ac.uk/pdbsum/7sv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sv6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sv6 OCA], [https://pdbe.org/7sv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sv6 RCSB], [https://www.ebi.ac.uk/pdbsum/7sv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sv6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C1JZ07_PAEAL C1JZ07_PAEAL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Evans, S V~~]]	+	[[Category: Paenibacillus alvei]]
-	[[Category: ~~Legg, M S.G~~]]	+	[[Category: Evans SV]]
-	[[Category: ~~S-layer]]~~	+	[[Category: Legg MSG]]
-	~~[[Category: Secondary cell wall polymer]]~~	+
-	~~[[Category: Slh domain]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 10:38, 6 April 2022

OCA — Wed, 06 Apr 2022 10:38:22 GMT

←Older revision		Revision as of 10:38, 6 April 2022
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	==Crystal structure of SpaA-SLH/G46A/G109A in complex with 4,6-Pyr-beta-D-ManNAc-(1->4)-beta-D-GlcNAcOMe==		==Crystal structure of SpaA-SLH/G46A/G109A in complex with 4,6-Pyr-beta-D-ManNAc-(1->4)-beta-D-GlcNAcOMe==
-	<StructureSection load='7sv6' size='340' side='right'caption='[[7sv6]]' scene=''>	+	<StructureSection load='7sv6' size='340' side='right'caption='[[7sv6]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SV6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7sv6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SV6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sv6 OCA], [https://pdbe.org/7sv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sv6 RCSB], [https://www.ebi.ac.uk/pdbsum/7sv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sv6 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D2Y:methyl+2-acetamido-4-O-{2-acetamido-4,6-O-[(1S)-1-carboxyethylidene]-2-deoxy-beta-D-mannopyranosyl}-2-deoxy-beta-D-glucopyranoside'>D2Y</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7sv3\|7sv3]], [[7sv5\|7sv5]], [[7sv4\|7sv4]]</div></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sv6 OCA], [https://pdbe.org/7sv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sv6 RCSB], [https://www.ebi.ac.uk/pdbsum/7sv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sv6 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Self-assembling (glyco)protein surface layers (S-layers) are ubiquitous prokaryotic cell-surface structures involved in structural maintenance, nutrient diffusion, host adhesion, virulence, and other processes, which makes them appealing targets for therapeutics and biotechnological applications as biosensors or drug delivery systems. However, unlocking this potential requires expanding our understanding of S-layer properties, especially the details of surface-attachment. S-layers of Gram-positive bacteria often are attached through the interaction of S-layer homology (SLH) domain trimers with peptidoglycan-linked secondary cell wall polymers (SCWPs). Co-crystal structures of the SLH domain trimer from the Paenibacillus alvei S-layer protein SpaA (SpaASLH) with synthetic, terminal SCWP disaccharide and trisaccharide analogues, together with isothermal titration calorimetry binding analyses, reveal that while SpaASLH accommodates longer biologically relevant SCWP ligands within both its primary (G2) and secondary (G1) binding sites, the terminal pyruvylated ManNAc moiety serves as the nearly-exclusive SCWP anchoring point. Binding is accompanied by displacement of a flexible loop adjacent to the receptor site that enhances the complementarity between protein and ligand, including electrostatic complementarity with the terminal pyruvate moiety. Remarkably, binding of the pyruvylated monosaccharide SCWP fragment alone is sufficient to cause rearrangement of the receptor binding sites in a manner necessary to accommodate longer SCWP fragments. The observation of multiple conformations in longer oligosaccharides bound to the protein, together with the demonstrated functionality of two of the three SCWP receptor binding sites, reveals how the SpaASLH-SCWP interaction has evolved to accommodate longer SCWP ligands and alleviate the strain inherent to bacterial S-layer adhesion during growth and division.
		+
		+	The S-layer homology domains of Paenibacillus alvei surface protein SpaA bind to cell wall polysaccharide through the terminal monosaccharide residue.,Legg MSG, Hager-Mair FF, Krauter S, Gagnon SML, Lopez-Guzman A, Lim C, Blaukopf M, Kosma P, Schaffer C, Evans SV J Biol Chem. 2022 Feb 18:101745. doi: 10.1016/j.jbc.2022.101745. PMID:35189140<ref>PMID:35189140</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7sv6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Evans SV]]	+	[[Category: Evans, S V]]
-	[[Category: Legg ~~MSG~~]]	+	[[Category: Legg, M S.G]]
		+	[[Category: S-layer]]
		+	[[Category: Secondary cell wall polymer]]
		+	[[Category: Slh domain]]
		+	[[Category: Sugar binding protein]]

OCA at 06:07, 9 March 2022

OCA — Wed, 09 Mar 2022 06:07:52 GMT

←Older revision		Revision as of 06:07, 9 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7sv6 ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of SpaA-SLH/G46A/G109A in complex with 4,6-Pyr-beta-D-ManNAc-(1->4)-beta-D-GlcNAcOMe==
-		+	<StructureSection load='7sv6' size='340' side='right'caption='[[7sv6]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SV6 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sv6 OCA], [https://pdbe.org/7sv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sv6 RCSB], [https://www.ebi.ac.uk/pdbsum/7sv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sv6 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Evans SV]]
		+	[[Category: Legg MSG]]

OCA at 15:40, 15 December 2021

OCA — Wed, 15 Dec 2021 15:40:28 GMT

←Older revision		Revision as of 15:40, 15 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7sv6 is ON HOLD	+	The entry 7sv6 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7sv6" [edit=sysop:move=sysop]

OCA — Wed, 24 Nov 2021 11:02:17 GMT

Protected "7sv6" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:02, 24 November 2021

OCA: New page: '''Unreleased structure''' The entry 7sv6 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 24 Nov 2021 11:02:16 GMT

New page: '''Unreleased structure''' The entry 7sv6 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7sv6 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]