7sws - Revision history

OCA at 16:55, 18 October 2023

OCA — Wed, 18 Oct 2023 16:55:57 GMT

←Older revision		Revision as of 16:55, 18 October 2023
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	<StructureSection load='7sws' size='340' side='right'caption='[[7sws]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='7sws' size='340' side='right'caption='[[7sws]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7sws]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7sws]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Acropora_millepora Acropora millepora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>~~, ~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.642Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sws OCA], [https://pdbe.org/7sws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sws RCSB], [https://www.ebi.ac.uk/pdbsum/7sws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sws ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sws OCA], [https://pdbe.org/7sws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sws RCSB], [https://www.ebi.ac.uk/pdbsum/7sws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sws ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q66PV0_ACRMI Q66PV0_ACRMI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Acropora millepora]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ahmed, H]]	+	[[Category: Ahmed H]]
-	[[Category: Caputo~~, A T~~]]	+	[[Category: Caputo AT]]
-	[[Category: Newman, J]]	+	[[Category: Newman J]]
-	[[Category: Scott, C]]	+	[[Category: Scott C]]
-	~~[[Category: Chromoprotein]]~~	+
-	~~[[Category: Fluorescent protein~~]]	+

OCA at 10:26, 18 May 2022

OCA — Wed, 18 May 2022 10:26:28 GMT

←Older revision		Revision as of 10:26, 18 May 2022
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	==Crystal structure of the chromoprotein amilCP==		==Crystal structure of the chromoprotein amilCP==
-	<StructureSection load='7sws' size='340' side='right'caption='[[7sws]]' scene=''>	+	<StructureSection load='7sws' size='340' side='right'caption='[[7sws]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7sws]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sws OCA], [https://pdbe.org/7sws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sws RCSB], [https://www.ebi.ac.uk/pdbsum/7sws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sws ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sws OCA], [https://pdbe.org/7sws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sws RCSB], [https://www.ebi.ac.uk/pdbsum/7sws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sws ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Anthozoan chromoproteins are highly pigmented, diversely coloured and readily produced in recombinant expression systems. While they are a versatile and powerful building block in synthetic biology for applications such as biosensor development, they are not widely used in comparison to the related fluorescent proteins, partly due to a lack of structural characterization to aid protein engineering. Here, high-resolution X-ray crystal structures of four open-source chromoproteins, gfasPurple, amilCP, spisPink and eforRed, are presented. These proteins are dimers in solution, and mutation at the conserved dimer interface leads to loss of visible colour development in gfasPurple. The chromophores are trans and noncoplanar in gfasPurple, amilCP and spisPink, while that in eforRed is cis and noncoplanar, and also emits fluorescence. Like other characterized chromoproteins, gfasPurple, amilCP and eforRed contain an sp(2)-hybridized N-acylimine in the peptide bond preceding the chromophore, while spisPink is unusual and demonstrates a true sp(3)-hybridized trans-peptide bond at this position. It was found that point mutations at the chromophore-binding site in gfasPurple that substitute similar amino acids to those in amilCP and spisPink generate similar colours. These features and observations have implications for the utility of these chromoproteins in protein engineering and synthetic biology applications.
		+
		+	Over the rainbow: structural characterization of the chromoproteins gfasPurple, amilCP, spisPink and eforRed.,Ahmed FH, Caputo AT, French NG, Peat TS, Whitfield J, Warden AC, Newman J, Scott C Acta Crystallogr D Struct Biol. 2022 May 1;78(Pt 5):599-612. doi:, 10.1107/S2059798322002625. Epub 2022 Apr 8. PMID:35503208<ref>PMID:35503208</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7sws" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ahmed H]]	+	[[Category: Ahmed, H]]
-	[[Category: Caputo AT]]	+	[[Category: Caputo, A T]]
-	[[Category: Newman J]]	+	[[Category: Newman, J]]
-	[[Category: Scott C]]	+	[[Category: Scott, C]]
		+	[[Category: Chromoprotein]]
		+	[[Category: Fluorescent protein]]

OCA at 02:56, 21 April 2022

OCA — Thu, 21 Apr 2022 02:56:18 GMT

←Older revision		Revision as of 02:56, 21 April 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7sws is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the chromoprotein amilCP==
-		+	<StructureSection load='7sws' size='340' side='right'caption='[[7sws]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWS FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sws OCA], [https://pdbe.org/7sws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sws RCSB], [https://www.ebi.ac.uk/pdbsum/7sws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sws ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ahmed H]]
		+	[[Category: Caputo AT]]
		+	[[Category: Newman J]]
		+	[[Category: Scott C]]

OCA at 08:34, 8 December 2021

OCA — Wed, 08 Dec 2021 08:34:32 GMT

←Older revision		Revision as of 08:34, 8 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7sws is ON HOLD	+	The entry 7sws is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7sws" [edit=sysop:move=sysop]

OCA — Wed, 01 Dec 2021 05:13:08 GMT

Protected "7sws" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:13, 1 December 2021

OCA: New page: '''Unreleased structure''' The entry 7sws is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 01 Dec 2021 05:13:07 GMT

New page: '''Unreleased structure''' The entry 7sws is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7sws is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]