7syr - Revision history

OCA at 04:16, 8 September 2022

OCA — Thu, 08 Sep 2022 04:16:06 GMT

←Older revision		Revision as of 04:16, 8 September 2022
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	==Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt).==		==Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt).==
-	<StructureSection load='7syr' size='340' side='right'caption='[[7syr]]' scene=''>	+	<StructureSection load='7syr' size='340' side='right'caption='[[7syr]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SYR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7syr]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SYR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7syr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7syr OCA], [https://pdbe.org/7syr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7syr RCSB], [https://www.ebi.ac.uk/pdbsum/7syr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7syr ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7syr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7syr OCA], [https://pdbe.org/7syr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7syr RCSB], [https://www.ebi.ac.uk/pdbsum/7syr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7syr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/G1U472_RABIT G1U472_RABIT]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hepatitis C virus mRNA contains an internal ribosome entry site (IRES) that mediates end-independent translation initiation, requiring a subset of eukaryotic initiation factors (eIFs). Biochemical studies revealed that direct binding of the IRES to the 40S ribosomal subunit places the initiation codon into the P site, where it base pairs with eIF2-bound Met-tRNAiMet forming a 48S initiation complex. Subsequently, eIF5 and eIF5B mediate subunit joining, yielding an elongation-competent 80S ribosome. Initiation can also proceed without eIF2, in which case Met-tRNAiMet is recruited directly by eIF5B. However, the structures of initiation complexes assembled on the HCV IRES, the transitions between different states, and the accompanying conformational changes have remained unknown. To fill these gaps, we now obtained cryo-EM structures of IRES initiation complexes, at resolutions up to 3.5 A, that cover all major stages from the initial ribosomal association, through eIF2-containing 48S initiation complexes, to eIF5B-containing complexes immediately prior to subunit joining. These structures provide insights into the dynamic network of 40S/IRES contacts, highlight the role of IRES domain II, and reveal conformational changes that occur during the transition from eIF2- to eIF5B-containing 48S complexes and prepare them for subunit joining.
		+
		+	Molecular architecture of 40S translation initiation complexes on the hepatitis C virus IRES.,Brown ZP, Abaeva IS, De S, Hellen CUT, Pestova TV, Frank J EMBO J. 2022 Aug 16;41(16):e110581. doi: 10.15252/embj.2022110581. Epub 2022 Jul , 13. PMID:35822879<ref>PMID:35822879</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7syr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Oryctolagus cuniculus]]
	[[Category: Abaeva IS]]		[[Category: Abaeva IS]]
	[[Category: Brown ZP]]		[[Category: Brown ZP]]

OCA at 11:08, 31 August 2022

OCA — Wed, 31 Aug 2022 11:08:34 GMT

←Older revision		Revision as of 11:08, 31 August 2022
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-	====	+	==Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt).==
	<StructureSection load='7syr' size='340' side='right'caption='[[7syr]]' scene=''>		<StructureSection load='7syr' size='340' side='right'caption='[[7syr]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SYR FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7syr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7syr OCA], [https://pdbe.org/7syr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7syr RCSB], [https://www.ebi.ac.uk/pdbsum/7syr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7syr ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7syr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7syr OCA], [https://pdbe.org/7syr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7syr RCSB], [https://www.ebi.ac.uk/pdbsum/7syr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7syr ProSAT]</span></td></tr>
	</table>		</table>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Abaeva IS]]
		+	[[Category: Brown ZP]]
		+	[[Category: De S]]
		+	[[Category: Frank J]]
		+	[[Category: Hellen CUT]]
		+	[[Category: Pestova TV]]

OCA at 18:04, 27 July 2022

OCA — Wed, 27 Jul 2022 18:04:53 GMT

←Older revision		Revision as of 18:04, 27 July 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7syr ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7syr' size='340' side='right'caption='[[7syr]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7syr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7syr OCA], [https://pdbe.org/7syr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7syr RCSB], [https://www.ebi.ac.uk/pdbsum/7syr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7syr ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 03:46, 20 July 2022

OCA — Wed, 20 Jul 2022 03:46:28 GMT

←Older revision		Revision as of 03:46, 20 July 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7syr is ON HOLD	+	The entry 7syr is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7syr" [edit=sysop:move=sysop]

OCA — Wed, 01 Dec 2021 05:16:20 GMT

Protected "7syr" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:16, 1 December 2021

OCA: New page: '''Unreleased structure''' The entry 7syr is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 01 Dec 2021 05:16:19 GMT

New page: '''Unreleased structure''' The entry 7syr is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7syr is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]