7syz - Revision history

OCA at 16:56, 18 October 2023

OCA — Wed, 18 Oct 2023 16:56:15 GMT

←Older revision		Revision as of 16:56, 18 October 2023
Line 1:		Line 1:

-	====	+	==Hendra virus G protein head domain in complex with cross-neutralizing murine antibody hAH1.3==
-	<StructureSection load='7syz' size='340' side='right'caption='[[7syz]]' scene=''>	+	<StructureSection load='7syz' size='340' side='right'caption='[[7syz]], [[Resolution\|resolution]] 2.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7syz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Hendra_henipavirus Hendra henipavirus] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SYZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SYZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7syz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7syz OCA], [https://pdbe.org/7syz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7syz RCSB], [https://www.ebi.ac.uk/pdbsum/7syz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7syz ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.86Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7syz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7syz OCA], [https://pdbe.org/7syz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7syz RCSB], [https://www.ebi.ac.uk/pdbsum/7syz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7syz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GLYCP_HENDH GLYCP_HENDH] Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of glycoprotein G to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hendra virus (HeV) and Nipah virus (NiV) are deadly zoonotic Henipaviruses (HNVs) responsible for recurrent outbreaks in humans and domestic species of highly fatal (50 to 95%) disease. A HeV variant (HeV-g2) of unprecedented genetic divergence has been identified in two fatally diseased horses, and in two flying fox species in regions of Australia not previously considered at risk for HeV spillover. Given the HeV-g2 divergence from HeV while retaining equivalent pathogenicity and spillover potential, understanding receptor usage and antigenic properties is urgently required to guide One Health biosecurity. Here, we show that the HeV-g2 G glycoprotein shares a conserved receptor tropism with prototypic HeV and that a panel of monoclonal antibodies recognizing the G and F glycoproteins potently neutralizes HeV-g2- and HeV G/F-mediated entry into cells. We determined a crystal structure of the Fab fragment of the hAH1.3 antibody bound to the HeV G head domain, revealing an antigenic site associated with potent cross-neutralization of both HeV-g2 and HeV. Structure-guided formulation of a tetravalent monoclonal antibody (mAb) mixture, targeting four distinct G head antigenic sites, results in potent neutralization of HeV and HeV-g2 and delineates a path forward for implementing multivalent mAb combinations for postexposure treatment of HNV infections.
		+
		+	Potent monoclonal antibody-mediated neutralization of a divergent Hendra virus variant.,Wang Z, Dang HV, Amaya M, Xu Y, Yin R, Yan L, Hickey AC, Annand EJ, Horsburgh BA, Reid PA, Smith I, Eden JS, Xu K, Broder CC, Veesler D Proc Natl Acad Sci U S A. 2022 May 31;119(22):e2122769119. doi: , 10.1073/pnas.2122769119. Epub 2022 May 26. PMID:35617431<ref>PMID:35617431</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7syz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hendra henipavirus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Mus musculus]]
		+	[[Category: Xu K]]
		+	[[Category: Xu Y]]

OCA at 05:30, 8 June 2022

OCA — Wed, 08 Jun 2022 05:30:13 GMT

←Older revision		Revision as of 05:30, 8 June 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7syz ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7syz' size='340' side='right'caption='[[7syz]]' scene=''>
-	~~Authors~~: ~~Xu, K~~.~~, Xu, Y~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Hendra virus G protein head domain in complex with cross~~-~~neutralizing murine antibody hAH1~~.3	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7syz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7syz OCA], [https://pdbe.org/7syz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7syz RCSB], [https://www.ebi.ac.uk/pdbsum/7syz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7syz ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Xu, K~~]]	+	__TOC__
-	[[Category: ~~Xu, Y~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 09:17, 5 January 2022

OCA — Wed, 05 Jan 2022 09:17:51 GMT

←Older revision		Revision as of 09:17, 5 January 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7syz is ON HOLD	+	The entry 7syz is ON HOLD until Paper Publication

	Authors: Xu, K., Xu, Y.		Authors: Xu, K., Xu, Y.
Line 7:		Line 7:
	Description: Hendra virus G protein head domain in complex with cross-neutralizing murine antibody hAH1.3		Description: Hendra virus G protein head domain in complex with cross-neutralizing murine antibody hAH1.3
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Xu, Y]]
	[[Category: Xu, K]]		[[Category: Xu, K]]
		+	[[Category: Xu, Y]]

OCA: Protected "7syz" [edit=sysop:move=sysop]

OCA — Wed, 01 Dec 2021 05:16:38 GMT

Protected "7syz" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:16, 1 December 2021

OCA: New page: '''Unreleased structure''' The entry 7syz is ON HOLD Authors: Xu, K., Xu, Y. Description: Hendra virus G protein head domain in complex with cross-neutralizing murine antibody hAH1.3 [...

OCA — Wed, 01 Dec 2021 05:16:37 GMT

New page: '''Unreleased structure''' The entry 7syz is ON HOLD Authors: Xu, K., Xu, Y. Description: Hendra virus G protein head domain in complex with cross-neutralizing murine antibody hAH1.3 [...

New page

'''Unreleased structure'''

The entry 7syz is ON HOLD

Authors: Xu, K., Xu, Y.

Description: Hendra virus G protein head domain in complex with cross-neutralizing murine antibody hAH1.3
[[Category: Unreleased Structures]]
[[Category: Xu, Y]]
[[Category: Xu, K]]