7szh - Revision history

OCA at 16:56, 18 October 2023

OCA — Wed, 18 Oct 2023 16:56:20 GMT

←Older revision		Revision as of 16:56, 18 October 2023
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	<StructureSection load='7szh' size='340' side='right'caption='[[7szh]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='7szh' size='340' side='right'caption='[[7szh]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7szh]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SZH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7szh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Microseira_wollei Microseira wollei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SZH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=D82:beta-Saxitoxinol'>D82</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.79Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=D82:beta-Saxitoxinol'>D82</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7szh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7szh OCA], [https://pdbe.org/7szh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7szh RCSB], [https://www.ebi.ac.uk/pdbsum/7szh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7szh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7szh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7szh OCA], [https://pdbe.org/7szh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7szh RCSB], [https://www.ebi.ac.uk/pdbsum/7szh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7szh ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C3RVQ0_9CYAN C3RVQ0_9CYAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Bridwell-Rabb, J~~]]	+	[[Category: Microseira wollei]]
-	[[Category: ~~Liu,~~ J]]	+	[[Category: Bridwell-Rabb J]]
-	[[Category: ~~Biosynthetic protein]]~~	+	[[Category: Liu J]]
-	~~[[Category: Saxitoxin gxta rieske oxygenase metalloprotein iron-sulfur cluster non-heme iron~~]]	+

OCA at 07:15, 27 January 2022

OCA — Thu, 27 Jan 2022 07:15:07 GMT

←Older revision		Revision as of 07:15, 27 January 2022
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	==Structure of the Rieske Non-heme Iron Oxygenase SxtT with beta-saxitoxinol Bound==		==Structure of the Rieske Non-heme Iron Oxygenase SxtT with beta-saxitoxinol Bound==
-	<StructureSection load='7szh' size='340' side='right'caption='[[7szh]]' scene=''>	+	<StructureSection load='7szh' size='340' side='right'caption='[[7szh]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SZH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7szh]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SZH FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7szh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7szh OCA], [https://pdbe.org/7szh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7szh RCSB], [https://www.ebi.ac.uk/pdbsum/7szh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7szh ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=D82:beta-Saxitoxinol'>D82</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7szh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7szh OCA], [https://pdbe.org/7szh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7szh RCSB], [https://www.ebi.ac.uk/pdbsum/7szh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7szh ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rieske oxygenases exploit the reactivity of iron to perform chemically challenging C-H bond functionalization reactions. Thus far, only a handful of Rieske oxygenases have been structurally characterized and remarkably little information exists regarding how these enzymes use a common architecture and set of metallocenters to facilitate a diverse range of reactions. Herein, we detail how two Rieske oxygenases SxtT and GxtA use different protein regions to influence the site-selectivity of their catalyzed monohydroxylation reactions. We present high resolution crystal structures of SxtT and GxtA with the native beta-saxitoxinol and saxitoxin substrates bound in addition to a Xenon-pressurized structure of GxtA that reveals the location of a substrate access tunnel to the active site. Ultimately, this structural information allowed for the identification of six residues distributed between three regions of SxtT that together control the selectivity of the C-H hydroxylation event. Substitution of these residues produces a SxtT variant that is fully adapted to exhibit the non-native site-selectivity and substrate scope of GxtA. Importantly, we also found that these selectivity regions are conserved in other structurally characterized Rieske oxygenases, providing a framework for predictively repurposing and manipulating Rieske oxygenases as biocatalysts.
		+
		+	Design principles for site-selective hydroxylation by a Rieske oxygenase.,Liu J, Tian J, Perry C, Lukowski AL, Doukov TI, Narayan ARH, Bridwell-Rabb J Nat Commun. 2022 Jan 11;13(1):255. doi: 10.1038/s41467-021-27822-3. PMID:35017498<ref>PMID:35017498</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7szh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bridwell-Rabb J]]	+	[[Category: Bridwell-Rabb, J]]
-	[[Category: Liu J]]	+	[[Category: Liu, J]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Saxitoxin gxta rieske oxygenase metalloprotein iron-sulfur cluster non-heme iron]]

OCA at 07:32, 19 January 2022

OCA — Wed, 19 Jan 2022 07:32:08 GMT

←Older revision		Revision as of 07:32, 19 January 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7szh ~~is ON HOLD until Paper Publication~~	+	==Structure of the Rieske Non-heme Iron Oxygenase SxtT with beta-saxitoxinol Bound==
-		+	<StructureSection load='7szh' size='340' side='right'caption='[[7szh]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SZH FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7szh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7szh OCA], [https://pdbe.org/7szh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7szh RCSB], [https://www.ebi.ac.uk/pdbsum/7szh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7szh ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bridwell-Rabb J]]
		+	[[Category: Liu J]]

OCA at 09:18, 5 January 2022

OCA — Wed, 05 Jan 2022 09:18:14 GMT

←Older revision		Revision as of 09:18, 5 January 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7szh is ON HOLD	+	The entry 7szh is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7szh" [edit=sysop:move=sysop]

OCA — Wed, 08 Dec 2021 08:35:26 GMT

Protected "7szh" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:35, 8 December 2021

OCA: New page: '''Unreleased structure''' The entry 7szh is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 08 Dec 2021 08:35:25 GMT

New page: '''Unreleased structure''' The entry 7szh is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7szh is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]